MB(ASCP) Exam Questions and
Answers 100% PASS
what does topoisomerase do?—ANSWER-breaks phosphate backbone to "un" supercoil
DNA
Another name for Topoisomerase II—ANSWER-Gyrase
Helicase—ANSWER-Un"zips" DNA at replication fork
What makes up the primosome?—ANSWER-1. primase
2. helicase
3. DNA C
NASBA stands for...—ANSWER-nucleic acid sequence based amplification
NASBA—ANSWER-RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent polymerase (cDNA-->RNA)
RNAse H degrades original RNA template
Evaluated gene EXPRESSION—ANSWER-RNA
ratio for organic extraction—ANSWER-Phenol (50): Chloroform (49): isoamyl alcohol (1)
, phenol—ANSWER-denatures proteins, dissolves lipids
chloroform—ANSWER-assists in dissolving remaining lipids, gives a clear interphase between
aqueous phase
Long term storage of RNA—ANSWER-70% EtOH at -70 C
ratio for 28S to 18S subunits—ANSWER-2:1 (can see this in a gel)
A260—ANSWER-nucleic acids
A280—ANSWER-protein
A230—ANSWER-organic compounds, phenol
A270—ANSWER-phenol
optical density of ds DNA—ANSWER-50 ug/ml
optical density ssDNA—ANSWER-33 ug/ml
optical density RNA—ANSWER-40 ug/ml
DNA concentration is calculated:—ANSWER-A260 x dilution factor x 50 ug/ml
RNA concentration =—ANSWER-A260 x dilution factor x 40 ug/ml
DNA B—ANSWER-helicase
DNA G—ANSWER-primase
Processivity—ANSWER-# of dNTPs that a polymerase can incorporate continuously without
dissociating from primer template
fidelity—ANSWER-error rate of polymerase
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
Answers 100% PASS
what does topoisomerase do?—ANSWER-breaks phosphate backbone to "un" supercoil
DNA
Another name for Topoisomerase II—ANSWER-Gyrase
Helicase—ANSWER-Un"zips" DNA at replication fork
What makes up the primosome?—ANSWER-1. primase
2. helicase
3. DNA C
NASBA stands for...—ANSWER-nucleic acid sequence based amplification
NASBA—ANSWER-RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent polymerase (cDNA-->RNA)
RNAse H degrades original RNA template
Evaluated gene EXPRESSION—ANSWER-RNA
ratio for organic extraction—ANSWER-Phenol (50): Chloroform (49): isoamyl alcohol (1)
, phenol—ANSWER-denatures proteins, dissolves lipids
chloroform—ANSWER-assists in dissolving remaining lipids, gives a clear interphase between
aqueous phase
Long term storage of RNA—ANSWER-70% EtOH at -70 C
ratio for 28S to 18S subunits—ANSWER-2:1 (can see this in a gel)
A260—ANSWER-nucleic acids
A280—ANSWER-protein
A230—ANSWER-organic compounds, phenol
A270—ANSWER-phenol
optical density of ds DNA—ANSWER-50 ug/ml
optical density ssDNA—ANSWER-33 ug/ml
optical density RNA—ANSWER-40 ug/ml
DNA concentration is calculated:—ANSWER-A260 x dilution factor x 50 ug/ml
RNA concentration =—ANSWER-A260 x dilution factor x 40 ug/ml
DNA B—ANSWER-helicase
DNA G—ANSWER-primase
Processivity—ANSWER-# of dNTPs that a polymerase can incorporate continuously without
dissociating from primer template
fidelity—ANSWER-error rate of polymerase
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
