Molecular Biology (ASCP Exam)
Questions and Answers 100%
PASS
Removing Mg2 ions from PCR mix. Increase or decreases stringency?—ANSWER-Increase
Increasing Temperature increases or decreases stringency?—ANSWER-Increases
Increase probe length increases or decreases stringency?—ANSWER-Increases
increase probe complexity increases or decreases stringency?—ANSWER-Increases
lower salt concentration increases or decreases stringency?—ANSWER-Increases
Less time increases or decreases stringency?—ANSWER-Increases
adding formamide increases or decreases stringency?—ANSWER-Decreases
adding bovine serum increases or decreases stringency?—ANSWER-Decreases
Increasing probe concentration increases or decreases stringency?—ANSWER-Decreases
PCR Inhibitors—ANSWER-Hemoglobin, Heparin, Xylene, Pheonl, Cyanol, and Bromophenal
blue
Preferred Tubes for molecular testing?—ANSWER-Lavender(EDTA) and Yellow(ACD=Acid
Citrate Dextrose)
, Specialized tubes for molecular testing?—ANSWER-PPT=Plasma Preparation Tube- designed
to separate lymphocytes/granulocytes for ease of DNA isolation extractions
CML Translocation & Method of Detection?—ANSWER-11:22 or 9:22, Fish/Cish
Philedelphia chromosome disorder?—ANSWER-CML
Ewings Sarcoma (Solid Tumor) Translocation & Method of detection?—ANSWER-11:22,
RTPCR
ALL Translocation & Method of detection?—ANSWER-9:22, FISH/CISH:/RTPCR
Follicular Lymphoma Translocation & Method of detection?—ANSWER-14:18, FISH/RTPCR
PML Translocation & Method of detection?—ANSWER-15:17, FISH/ RTPCR
Mantle Cell Lymphoma Translocation & Method of detection?—ANSWER-11:14,
FISH/PCR/RT-PCR
Burkitt Lymphoma Translocation & Method of detection?—ANSWER-8:14,FISH/CISH
Rhabdomyosarcoma (RMS/ARMS)Translocation & Method of detection?—ANSWER-2:13 or
1:13, RTPCR/PCR/ FISH
HER2/neu Breast Cancer Chromosome & method of Detection?—ANSWER-17, IHC:FISH:CISH
Tumor Protein 53 Chromosome & method of Detection?—ANSWER-17, SSCP: DS
BRCA1/2 Breast Cancer Chromosome & method of Detection?—ANSWER-17/13, SSCP: DS
α thalassemia Chromosome & method of Detection?—ANSWER-16, SSCP: DS
Prader Willi Syndrome Chromosome & method of Detection?—ANSWER-15, DS
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
Questions and Answers 100%
PASS
Removing Mg2 ions from PCR mix. Increase or decreases stringency?—ANSWER-Increase
Increasing Temperature increases or decreases stringency?—ANSWER-Increases
Increase probe length increases or decreases stringency?—ANSWER-Increases
increase probe complexity increases or decreases stringency?—ANSWER-Increases
lower salt concentration increases or decreases stringency?—ANSWER-Increases
Less time increases or decreases stringency?—ANSWER-Increases
adding formamide increases or decreases stringency?—ANSWER-Decreases
adding bovine serum increases or decreases stringency?—ANSWER-Decreases
Increasing probe concentration increases or decreases stringency?—ANSWER-Decreases
PCR Inhibitors—ANSWER-Hemoglobin, Heparin, Xylene, Pheonl, Cyanol, and Bromophenal
blue
Preferred Tubes for molecular testing?—ANSWER-Lavender(EDTA) and Yellow(ACD=Acid
Citrate Dextrose)
, Specialized tubes for molecular testing?—ANSWER-PPT=Plasma Preparation Tube- designed
to separate lymphocytes/granulocytes for ease of DNA isolation extractions
CML Translocation & Method of Detection?—ANSWER-11:22 or 9:22, Fish/Cish
Philedelphia chromosome disorder?—ANSWER-CML
Ewings Sarcoma (Solid Tumor) Translocation & Method of detection?—ANSWER-11:22,
RTPCR
ALL Translocation & Method of detection?—ANSWER-9:22, FISH/CISH:/RTPCR
Follicular Lymphoma Translocation & Method of detection?—ANSWER-14:18, FISH/RTPCR
PML Translocation & Method of detection?—ANSWER-15:17, FISH/ RTPCR
Mantle Cell Lymphoma Translocation & Method of detection?—ANSWER-11:14,
FISH/PCR/RT-PCR
Burkitt Lymphoma Translocation & Method of detection?—ANSWER-8:14,FISH/CISH
Rhabdomyosarcoma (RMS/ARMS)Translocation & Method of detection?—ANSWER-2:13 or
1:13, RTPCR/PCR/ FISH
HER2/neu Breast Cancer Chromosome & method of Detection?—ANSWER-17, IHC:FISH:CISH
Tumor Protein 53 Chromosome & method of Detection?—ANSWER-17, SSCP: DS
BRCA1/2 Breast Cancer Chromosome & method of Detection?—ANSWER-17/13, SSCP: DS
α thalassemia Chromosome & method of Detection?—ANSWER-16, SSCP: DS
Prader Willi Syndrome Chromosome & method of Detection?—ANSWER-15, DS
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
