The pull down assay described in Tutorial 7 is similar to co- The co-IP assay utilizes antibody specific to the target protein.
immunoprecipitation technique described earlier in this
course. Which of the following are differences between the AND
IP/co-IP techniques and the pull down assay? Select all that
apply.
You are creating a tagged protein fragment for use in a pull an NLS sequence
down assay as depicted in Figure 2 of the reading in order to
isolate importin - α. What sequence should your fragment
contain?
Which of the following is NOT a conserved eukaryotic GCTA box
promoter element ?
A/an i)________ element is a regulatory DNA sequence that binds i) enhancer; ii) upstream or downstream
transcription factors to increase transcriptional activation in a
gene specific manner. These sequences can be found
ii)__________ of the transcription start site.
Which of the following levels of gene regulation is most transcriptional regulation
frequently used in eukaryotic regulation?
Proteins that bind specific gene regulatory DNA sequences to Repressors
inhibit gene expression are called:
In order to use an electrophoretic (gel) mobility shift assay to labeled probe containing the sequence TATAAA
confirm that the initiation factor TFIID binds the TATA box of anti-TFIID antibody
the eukaryotic promoter which of the following reagents
would be required? Select all that apply.
In SDS-polyacrylamide gel electrophoresis, proteins are mass
resolved based on their:
, What is one thing that distinguishes SDS-PAGE from basic gel In SDS_PAGE an electric field is applied to the running gel.
electrophoresis (GE)?
A Western blot is a technique in which i)__________ molecules are i) Protein; ii) SDS-PAGE; iii) antibody
separated via ii)_________ , transferred to a membrane and
detected with labeled iii) _________.
Protein phosphorylation results in the i)___________ of the More than one of these responses is correct
phosphorylated protein.
i)________ add ii) __________ groups to target proteins, while a i) Kinases; ii) phosphate; iii) phosphatases
iii)________ remove them.
In Flow cytometry forward scatter is a measure of what size
cellular feature?
Which of the following reagents can be used to dye DNA for Promidium Iodine
flow cytometry?
Colcemid treatment arrests cells in i)___________ by ii) ______________. i) prophase; ii) inhibiting spindle assembly
Which of the following is a difference between the use of a a) when studying protein localization a fusion protein is created which consists of both
reporter gene to study gene regulation (ex. promoter deletion protein of interest and the reporter gene.
mapping with a reporter protein) and using a reporter gene to b) Unlike protein localization studies, when studying gene expression, only the
study protein localization? Select all that apply? reporter gene is expressed and not the actual gene of interest.
( any reporter gene can be used in both localization and expression constructs)
You are creating a tagged protein fragment for use in a pull NLS
down assay as depicted in Figure 2 of the reading in order to
isolate importin - α. What sequence should your fragment
contain?
What would happen if the STOP codon was not removed from The translated protein would not contain the GFP tag and GFP would not be translated at
the Open reading frame of the gene of interest before all.
insertion into a GFP plasmid like EGFP-N1 described in the
Tutorial 7 reading?
immunoprecipitation technique described earlier in this
course. Which of the following are differences between the AND
IP/co-IP techniques and the pull down assay? Select all that
apply.
You are creating a tagged protein fragment for use in a pull an NLS sequence
down assay as depicted in Figure 2 of the reading in order to
isolate importin - α. What sequence should your fragment
contain?
Which of the following is NOT a conserved eukaryotic GCTA box
promoter element ?
A/an i)________ element is a regulatory DNA sequence that binds i) enhancer; ii) upstream or downstream
transcription factors to increase transcriptional activation in a
gene specific manner. These sequences can be found
ii)__________ of the transcription start site.
Which of the following levels of gene regulation is most transcriptional regulation
frequently used in eukaryotic regulation?
Proteins that bind specific gene regulatory DNA sequences to Repressors
inhibit gene expression are called:
In order to use an electrophoretic (gel) mobility shift assay to labeled probe containing the sequence TATAAA
confirm that the initiation factor TFIID binds the TATA box of anti-TFIID antibody
the eukaryotic promoter which of the following reagents
would be required? Select all that apply.
In SDS-polyacrylamide gel electrophoresis, proteins are mass
resolved based on their:
, What is one thing that distinguishes SDS-PAGE from basic gel In SDS_PAGE an electric field is applied to the running gel.
electrophoresis (GE)?
A Western blot is a technique in which i)__________ molecules are i) Protein; ii) SDS-PAGE; iii) antibody
separated via ii)_________ , transferred to a membrane and
detected with labeled iii) _________.
Protein phosphorylation results in the i)___________ of the More than one of these responses is correct
phosphorylated protein.
i)________ add ii) __________ groups to target proteins, while a i) Kinases; ii) phosphate; iii) phosphatases
iii)________ remove them.
In Flow cytometry forward scatter is a measure of what size
cellular feature?
Which of the following reagents can be used to dye DNA for Promidium Iodine
flow cytometry?
Colcemid treatment arrests cells in i)___________ by ii) ______________. i) prophase; ii) inhibiting spindle assembly
Which of the following is a difference between the use of a a) when studying protein localization a fusion protein is created which consists of both
reporter gene to study gene regulation (ex. promoter deletion protein of interest and the reporter gene.
mapping with a reporter protein) and using a reporter gene to b) Unlike protein localization studies, when studying gene expression, only the
study protein localization? Select all that apply? reporter gene is expressed and not the actual gene of interest.
( any reporter gene can be used in both localization and expression constructs)
You are creating a tagged protein fragment for use in a pull NLS
down assay as depicted in Figure 2 of the reading in order to
isolate importin - α. What sequence should your fragment
contain?
What would happen if the STOP codon was not removed from The translated protein would not contain the GFP tag and GFP would not be translated at
the Open reading frame of the gene of interest before all.
insertion into a GFP plasmid like EGFP-N1 described in the
Tutorial 7 reading?
