In order to use a promoter plasmid to study the regulatory d) promoter region; upstream of the reporter gene
elements of a gene of interest, the _________ of your gene of
interest is inserted into the reporter plasmid _________.
a) open reading frame; upstream of the reporter gene
b) exons; upstream of the plasmid reporter
c) coding region; downstream of the plasmid reporter
d) promoter region; upstream of the reporter gene
e) structural region; downstream of the reporter gene
Which element within the eukaryotic promoter has the most GC box
significant impact of transcription when deleted?
Relative promoter activity after deletion....
TATA box- 38%
-40 to -70 region- 95%
GC box- 14%
CAAT box- 50%
-110 to -130 region- 114%
Sex determination in Drosophila embryos and post b) Alternate splicing
transcriptional regulation of the sxl, tra, and dsx transcripts are
an example of:
a) Non-disjunction
b) Alternate splicing
c) Gene expression
d) Transcriptional regulation
Which of the following RNA molecule binds perfectly to b) siRNA
mRNA leading to it's rapid degradation?
Question 2 options:
a) miRNA
b) siRNA
c) tRNA
d) rRNA
,In eukaryotic cells, control of gene expression occurs at d) mRNA stability
multiple levels. Poly-A tail length represents an aspect of
which level of control?
a) mRNA splicing
b) Post translational control
c) Transcriptional control
d) mRNA stability
The functional Sxl protein acts on which of the following pre- a) tra and c) sxl
mRNA transcripts to regulate sex determination in Drosphila
embryos? Select all that apply.
a) tra
b) Tra2
c) sxl
d) dsx
Complete the following sentences regarding the sex a) tra 1-3 splice transcript, c) dsx 3-5 splice transcript, b) dsx 3-4 splice transcript
determination in Drosophila embryos with the appropriate
selection from the list provided. Note some matches may be
used more than once.
Functional Slx protein acts on the tra pre-mRNA transcript to
produce the _____________ .
In the absence of functional Sxl protein the ____________ is
produced in Drosophila.
The presence of Tra protein leads to the production of the
___________ .
a) tra 1-3 splice transcript
b) dsx 3-4 splice transcript
c) dsx 3-5 splice transcript
d) slx 2-4 splice transcript
, Which of the following statements regarding miRNA and a) Both siRNA and miRNA act to inhibit or reduce translation of target mRNA. and d)
siRNA mediated post transcriptional regulation are correct. siRNA bind perfectly to the target mRNA while miRNA bind imperfectly.
Select all that apply.
a) Both siRNA and miRNA act to inhibit or reduce translation of
target mRNA.
b) RISC only associates with siRNA to aid in the destruction of
target mRNA.
c) Both siRNA and miRNA lead to the rapid destruction of
bound target mRNA.
d) siRNA bind perfectly to the target mRNA while miRNA bind
imperfectly.
What is one thing that distinguishes reverse transcriptase PCR b) The use of mRNA rather than DNA as an original template.
(RT-PCR) from quantitative PCR (qPCR)?
a) The ability to exponentially amplify DNA copy number.
b) The use of mRNA rather than DNA as an original template.
c) The use of temperature resistant taq DNA polymerase.
d) The use of DNA primers to replicate specific sequences.
The process of quantitative PCR (qPCR) utilizes ___________ in c) Fluorescently labeled dsDNA
order to monitor and quantify the amplification of the target
DNA.
a) Taq DNA polymerase
b) Fluorescently labeled ddNTPs
c) Fluorescently labeled dsDNA
d) Reverse Transcriptase
Which of the following reagents are used in a qPCR reaction? a, c, d, e, g
Select all that apply.
a) Reverse transcriptase
b) taq DNA polymerase
c) ddNTPs
d) DNA primers
e) Fluorescent dsDNA binding dye
f) Fluorescently labeled dNTPs
g) dNTPs
elements of a gene of interest, the _________ of your gene of
interest is inserted into the reporter plasmid _________.
a) open reading frame; upstream of the reporter gene
b) exons; upstream of the plasmid reporter
c) coding region; downstream of the plasmid reporter
d) promoter region; upstream of the reporter gene
e) structural region; downstream of the reporter gene
Which element within the eukaryotic promoter has the most GC box
significant impact of transcription when deleted?
Relative promoter activity after deletion....
TATA box- 38%
-40 to -70 region- 95%
GC box- 14%
CAAT box- 50%
-110 to -130 region- 114%
Sex determination in Drosophila embryos and post b) Alternate splicing
transcriptional regulation of the sxl, tra, and dsx transcripts are
an example of:
a) Non-disjunction
b) Alternate splicing
c) Gene expression
d) Transcriptional regulation
Which of the following RNA molecule binds perfectly to b) siRNA
mRNA leading to it's rapid degradation?
Question 2 options:
a) miRNA
b) siRNA
c) tRNA
d) rRNA
,In eukaryotic cells, control of gene expression occurs at d) mRNA stability
multiple levels. Poly-A tail length represents an aspect of
which level of control?
a) mRNA splicing
b) Post translational control
c) Transcriptional control
d) mRNA stability
The functional Sxl protein acts on which of the following pre- a) tra and c) sxl
mRNA transcripts to regulate sex determination in Drosphila
embryos? Select all that apply.
a) tra
b) Tra2
c) sxl
d) dsx
Complete the following sentences regarding the sex a) tra 1-3 splice transcript, c) dsx 3-5 splice transcript, b) dsx 3-4 splice transcript
determination in Drosophila embryos with the appropriate
selection from the list provided. Note some matches may be
used more than once.
Functional Slx protein acts on the tra pre-mRNA transcript to
produce the _____________ .
In the absence of functional Sxl protein the ____________ is
produced in Drosophila.
The presence of Tra protein leads to the production of the
___________ .
a) tra 1-3 splice transcript
b) dsx 3-4 splice transcript
c) dsx 3-5 splice transcript
d) slx 2-4 splice transcript
, Which of the following statements regarding miRNA and a) Both siRNA and miRNA act to inhibit or reduce translation of target mRNA. and d)
siRNA mediated post transcriptional regulation are correct. siRNA bind perfectly to the target mRNA while miRNA bind imperfectly.
Select all that apply.
a) Both siRNA and miRNA act to inhibit or reduce translation of
target mRNA.
b) RISC only associates with siRNA to aid in the destruction of
target mRNA.
c) Both siRNA and miRNA lead to the rapid destruction of
bound target mRNA.
d) siRNA bind perfectly to the target mRNA while miRNA bind
imperfectly.
What is one thing that distinguishes reverse transcriptase PCR b) The use of mRNA rather than DNA as an original template.
(RT-PCR) from quantitative PCR (qPCR)?
a) The ability to exponentially amplify DNA copy number.
b) The use of mRNA rather than DNA as an original template.
c) The use of temperature resistant taq DNA polymerase.
d) The use of DNA primers to replicate specific sequences.
The process of quantitative PCR (qPCR) utilizes ___________ in c) Fluorescently labeled dsDNA
order to monitor and quantify the amplification of the target
DNA.
a) Taq DNA polymerase
b) Fluorescently labeled ddNTPs
c) Fluorescently labeled dsDNA
d) Reverse Transcriptase
Which of the following reagents are used in a qPCR reaction? a, c, d, e, g
Select all that apply.
a) Reverse transcriptase
b) taq DNA polymerase
c) ddNTPs
d) DNA primers
e) Fluorescent dsDNA binding dye
f) Fluorescently labeled dNTPs
g) dNTPs
