PhysioEx Lab Report Exercise 12: Serological Testing Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA)

2/20/26, 12:39 PM PhysioEx Exercise 12 Activity 3



PhysioEx Lab Report
Exercise 12: Serological Testing
Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA)
Name: Morris
Date: 20 February 2026
Session ID:

Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1 For home pregnancy tests

You correctly answered: the antigen is human chorionic gonadotropin.

2 Which of the following is true of the indirect ELISA?

You correctly answered: A positive patient sample contains antibodies specific to the antigen
of interest.

3 What does the antigen bind to on the antibody?

You correctly answered: the variable region.

4 Which of the following is true of the direct ELISA?

You correctly answered: The positive patient sample contains the specific antigen.


Experiment Results
Predict Questions
No Predict Questions
Stop & Think Questions
1 What must occur in order for an HIV-infected individual to test positive with the indirect
ELISA test?

You correctly answered: seroconversion.

2 What is the key substance found in the developing buffer?

You correctly answered: enzyme linked to the secondary antibody.

Experiment Data

Sample Optical Density HIV Test Result

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