CERTIFICATION EXAM PRACTICE QUESTIONS AND
ANSWERS: THE MOST RECENT AND COMPREHENSIVE
VERSION WITH VERIFIED ANSWERS; GUARANTEED PASS
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Examination Overview
This comprehensive 150-question assessment is designed to mirror the format and rigor of national Sterile
Processing Technician certification exams (such as those aligned with standards from International
Association of Healthcare Central Service Materiel Management (HSPA), Certification Board for Sterile
Processing and Distribution, Association for the Advancement of Medical Instrumentation, Centers for
Disease Control and Prevention, and Occupational Safety and Health Administration).
The exam includes:
Section 1 (Questions 1–50): Foundational Sciences, Microbiology, Infection Prevention,
Regulations, Ethics, Safety, and Decontamination
Section 2 (Questions 51–100): Preparation & Packaging, Sterilization Methods, Monitoring,
Equipment, Instrumentation, Quality Assurance
Section 3 (Questions 101–150): Advanced Applications, Troubleshooting, Complex Devices,
Leadership, Documentation, Risk Management, Scenarios
1. The primary goal of the Sterile Processing Department (SPD) is to:
A. Reduce hospital supply costs
B. Maintain surgical schedules
C. Ensure delivery of safe, sterile, and functional medical devices
D. Supervise operating room staff
The core mission of SPD is patient safety through proper cleaning, disinfection,
sterilization, and distribution of medical devices.
2. Which microorganism type is MOST resistant to sterilization?
A. Vegetative bacteria
, B. Enveloped viruses
C. Fungi
D. Bacterial spores
Bacterial spores are highly resistant due to their protective structure and require
sterilization processes for destruction.
3. The chain of infection includes all EXCEPT:
A. Infectious agent
B. Reservoir
C. Antibiotic therapy
D. Mode of transmission
Antibiotic therapy is not a link in the chain of infection; it is a treatment method.
4. The minimum personal protective equipment (PPE) required in
decontamination includes:
A. Gloves only
B. Mask and gloves
C. Fluid-resistant gown, gloves, mask, and eye protection
D. Hair cover only
Full PPE protects against exposure to bloodborne pathogens and contaminated
fluids.
, 5. According to OSHA, exposure control plans must be reviewed:
A. Every 5 years
B. Every 3 years
C. Annually
D. Only after an incident
OSHA requires annual review and updates of exposure control plans.
6. The first step in instrument reprocessing is:
A. Sterilization
B. Packaging
C. Point-of-use cleaning
D. High-level disinfection
Removing gross soil at point of use prevents bioburden from drying and becoming
harder to remove.
7. Biofilm formation on instruments:
A. Improves sterilization
B. Is easily removed with water
C. Protects microorganisms from disinfectants and sterilants
D. Only occurs on plastic devices
Biofilms create a protective matrix that shields microorganisms.
, 8. Which water quality is recommended for final instrument rinse?
A. Tap water
B. Hard water
C. Distilled water only
D. Treated or deionized water
Treated water prevents mineral deposits and spotting.
9. The pH of most enzymatic detergents is:
A. Strongly acidic
B. Strongly alkaline
C. Neutral
D. Highly corrosive
Neutral pH enzymatic detergents protect instruments and break down organic soil.
10.The term “bioburden” refers to:
A. Sterility assurance level
B. Number of viable microorganisms on an item before sterilization
C. Endotoxins
D. Biofilm
Bioburden is the microbial load prior to sterilization.