1
BIOCHEM ACS Final Exam Questions
with Figures and Correct Answers
Exam Graded A+ 2026
Which of the following amino acids has a charged polar side chain at pH 7.0?
What is the charge?
A) Leu
B) Ala
C) Met
D) Trp
E) Glu
<Correct Answer>> E) Glu
Charge is negative 1
Which of the following amino acids does not have an ionizable side chain?
A) Asp B) Cys C) Lys D) His E) Asn
E) Asn
Page 1 of 46
,2
Which of the following amino acids has an uncharged polar side chain at pH
7.0?
A) Arg
B) Thr
C) Glu
D) Pro
E) Ile
<Correct Answer>> B) Thr
Page 2 of 46
,3
Which of the following tripeptides would be expected to be the most
hydrophobic?
A) KYG
B) KYA
C) GYA
D) DYA
E) DYG
<Correct Answer>> C) GYA
Page 3 of 46
, 4
You are trying to separate five proteins, which are listed below, by gel filtration
chromatography. Rank the proteins from first to last to elute.
A) cytochrome c (12 kDa)
B) RNA polymerase (99 kDa)
C) glutamine synthetase (621 kDa)
D) interferon-γ (34 kDa)
E) hemoglobin (62 kDa)
<Correct Answer>>) glutamine synthetase (621 kDa)
B) RNA polymerase (99 kDa)
E) hemoglobin (62 kDa)
D) interferon-γ (34 kDa)
A) cytochrome c (12 kDa)
C, B, E, D, A
My Note: in gel filtration chromatography, biggest molecules elute first,
smallest molecules elute last (because the small ones get stuck in the beads so
they can't elute as quickly).
Which of the following tripeptides would be expected to be the most
hydrophobic?
A) KYG
Page 4 of 46
BIOCHEM ACS Final Exam Questions
with Figures and Correct Answers
Exam Graded A+ 2026
Which of the following amino acids has a charged polar side chain at pH 7.0?
What is the charge?
A) Leu
B) Ala
C) Met
D) Trp
E) Glu
<Correct Answer>> E) Glu
Charge is negative 1
Which of the following amino acids does not have an ionizable side chain?
A) Asp B) Cys C) Lys D) His E) Asn
E) Asn
Page 1 of 46
,2
Which of the following amino acids has an uncharged polar side chain at pH
7.0?
A) Arg
B) Thr
C) Glu
D) Pro
E) Ile
<Correct Answer>> B) Thr
Page 2 of 46
,3
Which of the following tripeptides would be expected to be the most
hydrophobic?
A) KYG
B) KYA
C) GYA
D) DYA
E) DYG
<Correct Answer>> C) GYA
Page 3 of 46
, 4
You are trying to separate five proteins, which are listed below, by gel filtration
chromatography. Rank the proteins from first to last to elute.
A) cytochrome c (12 kDa)
B) RNA polymerase (99 kDa)
C) glutamine synthetase (621 kDa)
D) interferon-γ (34 kDa)
E) hemoglobin (62 kDa)
<Correct Answer>>) glutamine synthetase (621 kDa)
B) RNA polymerase (99 kDa)
E) hemoglobin (62 kDa)
D) interferon-γ (34 kDa)
A) cytochrome c (12 kDa)
C, B, E, D, A
My Note: in gel filtration chromatography, biggest molecules elute first,
smallest molecules elute last (because the small ones get stuck in the beads so
they can't elute as quickly).
Which of the following tripeptides would be expected to be the most
hydrophobic?
A) KYG
Page 4 of 46
