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BIOL 201 questions and
answers + rationales already
graded A+
1. A new organism is discovered deep in the earth’s crust whose basic
molecular biological mechanisms are similar to those of known
bacteria, except that its DNA and RNA contain only guanine and
cytosine heterocyclic bases. Which of the following properties
would we expect of its proteins?
a. They would contain a maximum of 8 different amino acids and
could include alpha-helix, not beta-sheet secondary structure
b. They would contain a maximum of 8 different amino acids and
could include both alpha-helix and beta-sheet secondary
structure
c. They would contain a maximum of 8 different amino acids and
could include alpha-helix, but not beta-sheet secondary
structure
d. They would contain a maximum of 8 different amino acids and
could include alpha-helix but not beta-sheet secondary
structure
e. They would contain a maximum of 4 different amino acids and
could include neither alpha-helix nor beta-sheet secondary
structure
2. DNA isolate from the bacterial virus M13 contains 25% A, 33% T,
22%, C 20%, G Which of the following is the most plausible
explanation?
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a. The base pairs in M13 DNA use wobble pairing rather than
standard pairing
b. A high percentage of the C residues are methylated at the 5
position
c. The DNA is in the form of a left-handed helix rather than the
usual right-handed helix
d. The viral DNA is subject to excessive recombination
e. The virus packages single-stranded, not double-stranded DNA
3. Which of the following is responsible for the initial recognition of
irretrievably misfolded or chemically damaged proteins in order for
them to be targeted for degradation
a. E1 Ubiquitin ligase
b. E2 ubiquitin ligase
c. E3 ubiquitin ligase
d. The proteasome cap
e. The proteasome core
4. Which of the following is responsible for catalyzing peptide bond
formation (i.e. peptidyl transferase activity) during eukaryotic
translation?
a. aminoacyl-tRNA synthetase
b. EIF2
c. EIF3
d. The large subunit rRNA
e. The anticodon
5. Based on the presence of conserved motifs in the protein amino acid
sequence, a scientist is convinced that protein X should be
associated with the outer surface of mitochondria within cells. She
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knows that mitochondria isolated by cell lysis and differential
centrifugation do not in fact contain protein X, but she proposes that,
during cell lysis, the protein dissociates from the mitochondrial
surface and is lost during mitochondrial isolation. Which of the
following experimental approaches are likely to be useful to her in
gathering evidence to support her hypothesis? More than one
answer is correct.
a. Mass spec of whole cells and isolated molecules
b. Fluorescence microscopy of living cells transfected with a
recombinant DNA construct expressing a fusion protein
consisting of protein x fused to the green fluorescent protein
c. Western blotting of whole cells and isolated mitochondria
using an anti protein X antibody an an appropriately labeled
secondary antibody
d. Immunofluorescence microscopy of fixed cells using an anti
protein X antibody and an appropriate fluorescent secondary
antibody
e. Northern blotting of a whole cell RNA using a cDNA clone of
the protein mrna as the hybridization probe
6. Which of the following are examples of allosteric regulation? (select
all that apply)
a. The cleavage of a enzyme into inactive fragments by a protease
b. Inhibition of an enzyme’s ability to bind its substrate by the
presence of a chemically related molecule that binds to the
active site
c. A large-scale change in a protein’s tertiary structure upon
binding an ion 9e.g.
Ca2+) or a low molecular weight molecule (ATP)
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BIOL 201 questions and
answers + rationales already
graded A+
1. A new organism is discovered deep in the earth’s crust whose basic
molecular biological mechanisms are similar to those of known
bacteria, except that its DNA and RNA contain only guanine and
cytosine heterocyclic bases. Which of the following properties
would we expect of its proteins?
a. They would contain a maximum of 8 different amino acids and
could include alpha-helix, not beta-sheet secondary structure
b. They would contain a maximum of 8 different amino acids and
could include both alpha-helix and beta-sheet secondary
structure
c. They would contain a maximum of 8 different amino acids and
could include alpha-helix, but not beta-sheet secondary
structure
d. They would contain a maximum of 8 different amino acids and
could include alpha-helix but not beta-sheet secondary
structure
e. They would contain a maximum of 4 different amino acids and
could include neither alpha-helix nor beta-sheet secondary
structure
2. DNA isolate from the bacterial virus M13 contains 25% A, 33% T,
22%, C 20%, G Which of the following is the most plausible
explanation?
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a. The base pairs in M13 DNA use wobble pairing rather than
standard pairing
b. A high percentage of the C residues are methylated at the 5
position
c. The DNA is in the form of a left-handed helix rather than the
usual right-handed helix
d. The viral DNA is subject to excessive recombination
e. The virus packages single-stranded, not double-stranded DNA
3. Which of the following is responsible for the initial recognition of
irretrievably misfolded or chemically damaged proteins in order for
them to be targeted for degradation
a. E1 Ubiquitin ligase
b. E2 ubiquitin ligase
c. E3 ubiquitin ligase
d. The proteasome cap
e. The proteasome core
4. Which of the following is responsible for catalyzing peptide bond
formation (i.e. peptidyl transferase activity) during eukaryotic
translation?
a. aminoacyl-tRNA synthetase
b. EIF2
c. EIF3
d. The large subunit rRNA
e. The anticodon
5. Based on the presence of conserved motifs in the protein amino acid
sequence, a scientist is convinced that protein X should be
associated with the outer surface of mitochondria within cells. She
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EMAIL:
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knows that mitochondria isolated by cell lysis and differential
centrifugation do not in fact contain protein X, but she proposes that,
during cell lysis, the protein dissociates from the mitochondrial
surface and is lost during mitochondrial isolation. Which of the
following experimental approaches are likely to be useful to her in
gathering evidence to support her hypothesis? More than one
answer is correct.
a. Mass spec of whole cells and isolated molecules
b. Fluorescence microscopy of living cells transfected with a
recombinant DNA construct expressing a fusion protein
consisting of protein x fused to the green fluorescent protein
c. Western blotting of whole cells and isolated mitochondria
using an anti protein X antibody an an appropriately labeled
secondary antibody
d. Immunofluorescence microscopy of fixed cells using an anti
protein X antibody and an appropriate fluorescent secondary
antibody
e. Northern blotting of a whole cell RNA using a cDNA clone of
the protein mrna as the hybridization probe
6. Which of the following are examples of allosteric regulation? (select
all that apply)
a. The cleavage of a enzyme into inactive fragments by a protease
b. Inhibition of an enzyme’s ability to bind its substrate by the
presence of a chemically related molecule that binds to the
active site
c. A large-scale change in a protein’s tertiary structure upon
binding an ion 9e.g.
Ca2+) or a low molecular weight molecule (ATP)
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