CHEM 123 Determination of Serum Albumin Lab Experiment Report

downloaded from




Scholarfriends




https://scholarfriends.com

Downloaded by: | https://scholarfriends.com/singlePaper/618246/chem-123-determination-of-serum-albumin-lab-
experiment-report
Distribution of this document is illegal.

, TA: Robert Zhang

Date: 9/27/17

Determination of Serum Albumin



1. Observations:
- When we add 20 drops of bromocresol green (BCG) into the solution of NaCl and

Albumin, the more albumin albumin compares to the other solutions (NaCl

+Albumin), the more it's greener.
- The least Albumin (the most NaCl) in the solution has the lightest green.
- The most Albumin (the least NaCl) in the solution has the darkest green.
- The absorbances also gradually increase as the albumin is getting more in the

solution.
- The color after adding BCG to the solution of NaCl + Albumin changed from

yellow-green to green-blue.



2. Data Tables: Wavelength: 635.7
a. Standard Solutions:

Wavelength: 635.7




Standard Diluted Volume of Volume of Absorbance
Concentration concentration Albumin NaCl solution
(g/L) (g/L) solution (mL) (mL)

20 0.50 2 10 - 2 = 8 0.35

30 0.75 3 10 - 3 = 7 0.43

40 1.00 4 10 - 4 = 6 0.63

50 1.25 5 10 - 5 = 5 0.70



This study source was downloaded by 100000900501157 from CourseHero.com on 02-24-2026 01:01:04 GMT -06:00


https://www.coursehero.com/file/30433698/Determination-of-Serum-Albumin/