Bio 99 Midterm 1 Lectures 1-6 Questions With Complete
Solutions
A and T has how many hydrogen bonds between them? Correct
Answers 2
A plasmid lacking an ORI will still be able to be transformed
into yeast, true or false? Correct Answers true! but no
replication!
Absorbance of DNA Correct Answers - double stranded DNA
will have little absorbance
-denatured DNA will have higher absorbance because light is
being allowed to pass through
Auxotroph Correct Answers a mutant organism (especially a
bacterium or fungus) that requires a particular additional nutrient
which the normal strain does not
Central Dogma Correct Answers DNA-transcription-RNA-
translation-protein
Centromere Correct Answers chromosome segregation, where
protein binds to ensure separation of daughter cells
Chargaff's Rule Correct Answers A=T and C=G
DNA denaturation Correct Answers In vivo: DNA helicase can
unwind the strands
In vitro: increase the temp
, DNA Helix Characteristics Correct Answers - composed of 2
DNA strands
- anti-parallel
- outside of the helix is a hydrophilic sugar phosphate backbone
- inside the helix are hydrophobic H-bonded bases
DNA polymerase requires: Correct Answers 1) DNA template
strand: DNA polymerase can only add to existing strands
2) RNA strand primer because RNA primer can begin a new
strand itself
3) dNTPs as substrates (dinucleotide triphosphates)
DNA vs RNA nucleotide structure Correct Answers RNA has
hydroxyl in 2- carbon which makes it less stable than DNA
Do plasmids need telomeres? Correct Answers no because they
do not need to worry about the end replication problem because
they are circular
Evidence of DNA packaging experiment Correct Answers -
chromatin + small amounts of nuclease (in order to cut DNA
and break phosphodiester bond
-separate DNA from protein and then run DNA on agarose gel
- the bands are DNA and they are getting compacted through
replication cycles
Exception to the Central Dogma Correct Answers RNA viruses:
they only have RNA meaning their Central Dogma is RNA to
protein
Solutions
A and T has how many hydrogen bonds between them? Correct
Answers 2
A plasmid lacking an ORI will still be able to be transformed
into yeast, true or false? Correct Answers true! but no
replication!
Absorbance of DNA Correct Answers - double stranded DNA
will have little absorbance
-denatured DNA will have higher absorbance because light is
being allowed to pass through
Auxotroph Correct Answers a mutant organism (especially a
bacterium or fungus) that requires a particular additional nutrient
which the normal strain does not
Central Dogma Correct Answers DNA-transcription-RNA-
translation-protein
Centromere Correct Answers chromosome segregation, where
protein binds to ensure separation of daughter cells
Chargaff's Rule Correct Answers A=T and C=G
DNA denaturation Correct Answers In vivo: DNA helicase can
unwind the strands
In vitro: increase the temp
, DNA Helix Characteristics Correct Answers - composed of 2
DNA strands
- anti-parallel
- outside of the helix is a hydrophilic sugar phosphate backbone
- inside the helix are hydrophobic H-bonded bases
DNA polymerase requires: Correct Answers 1) DNA template
strand: DNA polymerase can only add to existing strands
2) RNA strand primer because RNA primer can begin a new
strand itself
3) dNTPs as substrates (dinucleotide triphosphates)
DNA vs RNA nucleotide structure Correct Answers RNA has
hydroxyl in 2- carbon which makes it less stable than DNA
Do plasmids need telomeres? Correct Answers no because they
do not need to worry about the end replication problem because
they are circular
Evidence of DNA packaging experiment Correct Answers -
chromatin + small amounts of nuclease (in order to cut DNA
and break phosphodiester bond
-separate DNA from protein and then run DNA on agarose gel
- the bands are DNA and they are getting compacted through
replication cycles
Exception to the Central Dogma Correct Answers RNA viruses:
they only have RNA meaning their Central Dogma is RNA to
protein
