Bio 99 Pre-Lecture Quizzes Questions With Complete
Solutions
A deoxyribonucleotide is composed of (select all that apply):
Correct Answers Pentose with 3' OH
Phosphate group
Nitrogenous base
A DNA sequence of interest was digested and ligated into the
middle of the AmpR gene region of pBR322 vector. The vector
also contains a TetR gene which is not interrupted. After
introducing the recombinat DNA into bacterial cells, the
resulting transformants were tested on a variety of media. Which
of the following results would be expected? Correct Answers
Death on media containing both tetracyclin and ampicillin.
Growth on tetracyclin containing media.
A plasmid vector typically has which of the following features?
Select all that apply. Correct Answers A selectable marker to
ensure that the only cells containing the intact vector can grow.
An origin of replication.
Unique restriction sites for insertion of DNA.
Small size to facilitate entry into the cell and biochemical
manipulation of the DNA.
A polylinker is: Correct Answers synthetic DNA fragment that
contains multiple restriction enzyme sites
An appropriate dNTP forms a hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
between the 3' OH group of the 1st nucleotide and the 5'
, phosphate of the 2nd nucleotide. The enzyme responsible for
catalyzing formation of this bond is: Correct Answers DNA
polymerase
Arthur Kornberg's group found that in vitro DNA replication
required all four dNTPs. What conclusion did they arrive at
based on this result? Correct Answers The DNA synthesis
enzyme must be using a DNA strand as a template for synthesis.
Avery found that DNA was necessary and sufficient for
bacterial transformation. Which of the following experimental
results support this statement? Select all that apply. Correct
Answers Removal of DNA from cell extracts prevented
transformation.
DNA alone will cause transformation to occur.
Base excision repair is responsible for fixing (select all that
apply): Correct Answers individual damaged nucleotides that
minimally impact the helix structure.
single stranded breaks.
Because replication takes place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme: Correct Answers DNA ligase
Bidirectional synthesis was demonstrated by an experiment
involving radiolabeled dTTP in a pulse chase experiment.
Which result told the researchers that replication is bi-
Solutions
A deoxyribonucleotide is composed of (select all that apply):
Correct Answers Pentose with 3' OH
Phosphate group
Nitrogenous base
A DNA sequence of interest was digested and ligated into the
middle of the AmpR gene region of pBR322 vector. The vector
also contains a TetR gene which is not interrupted. After
introducing the recombinat DNA into bacterial cells, the
resulting transformants were tested on a variety of media. Which
of the following results would be expected? Correct Answers
Death on media containing both tetracyclin and ampicillin.
Growth on tetracyclin containing media.
A plasmid vector typically has which of the following features?
Select all that apply. Correct Answers A selectable marker to
ensure that the only cells containing the intact vector can grow.
An origin of replication.
Unique restriction sites for insertion of DNA.
Small size to facilitate entry into the cell and biochemical
manipulation of the DNA.
A polylinker is: Correct Answers synthetic DNA fragment that
contains multiple restriction enzyme sites
An appropriate dNTP forms a hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
between the 3' OH group of the 1st nucleotide and the 5'
, phosphate of the 2nd nucleotide. The enzyme responsible for
catalyzing formation of this bond is: Correct Answers DNA
polymerase
Arthur Kornberg's group found that in vitro DNA replication
required all four dNTPs. What conclusion did they arrive at
based on this result? Correct Answers The DNA synthesis
enzyme must be using a DNA strand as a template for synthesis.
Avery found that DNA was necessary and sufficient for
bacterial transformation. Which of the following experimental
results support this statement? Select all that apply. Correct
Answers Removal of DNA from cell extracts prevented
transformation.
DNA alone will cause transformation to occur.
Base excision repair is responsible for fixing (select all that
apply): Correct Answers individual damaged nucleotides that
minimally impact the helix structure.
single stranded breaks.
Because replication takes place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme: Correct Answers DNA ligase
Bidirectional synthesis was demonstrated by an experiment
involving radiolabeled dTTP in a pulse chase experiment.
Which result told the researchers that replication is bi-
