Bio 99 Exam 2 Questions With Complete Solutions
A plasmid vector typically has which of the following features?
Select all that apply.
a) Small size to facilitate entry into the cell and biochemical
manipulation of the DNA.
b) Unique restriction sites for insertion of DNA.
c) A selectable marker to ensure that the only cells containing
the intact vector can grow.
d) An origin of replication. Correct Answers a, b, c & d
A polylinker is:
a) A synthetic fragment used in colony hybridization to visualize
colonies that contain the DNA of interest.
b) A synthetic fragment that contains multiple sites for
restriction enzymes to cut the DNA.
c) An enzyme used to connect DNA fragments together.
d) A sequence that allows for selection of cells that contain the
vector.
e) A synthetic fragment inserted into a vector that allows
replication of the vector in the host cell. Correct Answers b
After 10 cycles of a PCR reaction, how much have you
amplified your starting template DNA by? Select the best
answer.
a) by 20
b) by 10
c) by 2^10 Correct Answers c
An appropriate dNTP forms a hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
, between the 3' OH group of the 1st nucleotide and the 5'
phosphate of the 2nd nucleotide. The enzyme responsible for
catalyzing the formation of this bond is:
a) DNA polymerase
b) primase
c) DNA helicase
d) Topoisomerase
e) DNA ligase Correct Answers a
Base excision repair is responsible for fixing (select all that
apply):
a) double stranded breaks.
b) single stranded breaks.
c) individual damaged nucleotides that minimally impact the
helix structure.
d) individual damaged nucleotides that cause a major impact to
the helix structure. Correct Answers b & c
Because replication takes place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme:
a) DNA polymerase
b) primase
c) DNA helicase
d) Topoisomerase
e) DNA ligase Correct Answers e
Different types of mutations include silent, missense and
nonsense. Match each with the descriptions below:
A plasmid vector typically has which of the following features?
Select all that apply.
a) Small size to facilitate entry into the cell and biochemical
manipulation of the DNA.
b) Unique restriction sites for insertion of DNA.
c) A selectable marker to ensure that the only cells containing
the intact vector can grow.
d) An origin of replication. Correct Answers a, b, c & d
A polylinker is:
a) A synthetic fragment used in colony hybridization to visualize
colonies that contain the DNA of interest.
b) A synthetic fragment that contains multiple sites for
restriction enzymes to cut the DNA.
c) An enzyme used to connect DNA fragments together.
d) A sequence that allows for selection of cells that contain the
vector.
e) A synthetic fragment inserted into a vector that allows
replication of the vector in the host cell. Correct Answers b
After 10 cycles of a PCR reaction, how much have you
amplified your starting template DNA by? Select the best
answer.
a) by 20
b) by 10
c) by 2^10 Correct Answers c
An appropriate dNTP forms a hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
, between the 3' OH group of the 1st nucleotide and the 5'
phosphate of the 2nd nucleotide. The enzyme responsible for
catalyzing the formation of this bond is:
a) DNA polymerase
b) primase
c) DNA helicase
d) Topoisomerase
e) DNA ligase Correct Answers a
Base excision repair is responsible for fixing (select all that
apply):
a) double stranded breaks.
b) single stranded breaks.
c) individual damaged nucleotides that minimally impact the
helix structure.
d) individual damaged nucleotides that cause a major impact to
the helix structure. Correct Answers b & c
Because replication takes place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme:
a) DNA polymerase
b) primase
c) DNA helicase
d) Topoisomerase
e) DNA ligase Correct Answers e
Different types of mutations include silent, missense and
nonsense. Match each with the descriptions below:
