Bio 99: Practice Quiz #1 Questions With Complete Solutions
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
Following her transformation of the plasmid into her yeast, what
media will the cells be plated on to select for cells that have
picked up the plasmid? Correct Answers Answer
-Media containing histidine
- Media lacking adenine
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She fixes the mistakes from the previous experiment and now
has a complete plasmid (selection marker, origin of replication,
centromere). She then inserts telomere sequences into the
plasmid. How will this impact her transformation Correct
Answers ???
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
,She starts by attempting to add the centromere DNA into a
plasmid containing the origin of replication. Unfortunately,
when adding the centromere sequence, the origin of replication
is removed, thus leaving a plasmid with only a centromere and
selection marker. Following plasmid transformation, what
growth result will she see on her plates? Correct Answers
Answer:
No colonies
Cystic fibrosis is caused by nonsense and missense mutations in
the CFTR gene, which encodes for a chloride channel. You are
studying cystic fibrosis patients to determine what mutation they
possess in the CFTR gene. The difference between the mutant
and wild type CFTR genes can be uncovered by examining the
CFTR: Correct Answers A. -DNA
B. -mRNA
C. -Protein
D. tRNA
Answer: A B C
Explain the 5' to 3' directionality of a DNA stand. Correct
Answers Answer:
It is due to the fact that the free 5' carbon is on one end and the
free 3' carbon is on the other
Griffith and Avery's transformation experiments allowed us to
identify that DNA is our genetic information. Which of the
following scenarios would result in bacterial cells that are
, capable of killing mice upon injection? Correct Answers
Answer:
Heat killed non-virulent bacteria is added to a live virulent
bacteria strain.
Imagine Hershey/Chase had used an RNA virus (genome
composed of RNA) instead of a DNA virus in their experiment.
Would radioactivity still have been found in the pellet? Correct
Answers Answer:
Yes, because DNA and RNA nucleotides are similar.
In the Meselson/Stahl experiment and shows the DNA bands
present after centrifugation. The bacteria start in 15N media and
are shifted to 14N at t=0. Each lane contains DNA extracted
from cells at various times (1 is earliest, 4 is latest). The DNA
bands present in each lane are highlighted with an arrow, as
some may be difficult to see.
If the extracted DNA was in very basic conditions,
(GREATER/FEWER/THE SAME) number of bands would be
seen in the photo after 1 round of replication compared to native
conditions. Correct Answers Answer:
Greater
In the Meselson/Stahl experiment, E. coli were first grown in
media containing heavy nitrogen, 15N, and then transferred to
light nitrogen, 14N at the beginning of the experiment.
What fraction of the DNA helices will consist of mixed DNA
after 4 rounds of replication? Correct Answers Answer:
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
Following her transformation of the plasmid into her yeast, what
media will the cells be plated on to select for cells that have
picked up the plasmid? Correct Answers Answer
-Media containing histidine
- Media lacking adenine
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She fixes the mistakes from the previous experiment and now
has a complete plasmid (selection marker, origin of replication,
centromere). She then inserts telomere sequences into the
plasmid. How will this impact her transformation Correct
Answers ???
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (∆ade) and histidine (∆his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
,She starts by attempting to add the centromere DNA into a
plasmid containing the origin of replication. Unfortunately,
when adding the centromere sequence, the origin of replication
is removed, thus leaving a plasmid with only a centromere and
selection marker. Following plasmid transformation, what
growth result will she see on her plates? Correct Answers
Answer:
No colonies
Cystic fibrosis is caused by nonsense and missense mutations in
the CFTR gene, which encodes for a chloride channel. You are
studying cystic fibrosis patients to determine what mutation they
possess in the CFTR gene. The difference between the mutant
and wild type CFTR genes can be uncovered by examining the
CFTR: Correct Answers A. -DNA
B. -mRNA
C. -Protein
D. tRNA
Answer: A B C
Explain the 5' to 3' directionality of a DNA stand. Correct
Answers Answer:
It is due to the fact that the free 5' carbon is on one end and the
free 3' carbon is on the other
Griffith and Avery's transformation experiments allowed us to
identify that DNA is our genetic information. Which of the
following scenarios would result in bacterial cells that are
, capable of killing mice upon injection? Correct Answers
Answer:
Heat killed non-virulent bacteria is added to a live virulent
bacteria strain.
Imagine Hershey/Chase had used an RNA virus (genome
composed of RNA) instead of a DNA virus in their experiment.
Would radioactivity still have been found in the pellet? Correct
Answers Answer:
Yes, because DNA and RNA nucleotides are similar.
In the Meselson/Stahl experiment and shows the DNA bands
present after centrifugation. The bacteria start in 15N media and
are shifted to 14N at t=0. Each lane contains DNA extracted
from cells at various times (1 is earliest, 4 is latest). The DNA
bands present in each lane are highlighted with an arrow, as
some may be difficult to see.
If the extracted DNA was in very basic conditions,
(GREATER/FEWER/THE SAME) number of bands would be
seen in the photo after 1 round of replication compared to native
conditions. Correct Answers Answer:
Greater
In the Meselson/Stahl experiment, E. coli were first grown in
media containing heavy nitrogen, 15N, and then transferred to
light nitrogen, 14N at the beginning of the experiment.
What fraction of the DNA helices will consist of mixed DNA
after 4 rounds of replication? Correct Answers Answer:
