Bio 99 Quiz Answers Questions With Complete Solutions
A complementary dNTP ("next nucleotide") forms a hydrogen
bond with an exposed base on the template DNA strand. Name
the enzyme catalyzing the bond formation between the 3' OH
group of the "preceeding nucleotide" and the 5' triphosphate of
the "next nucleotide".
p376 Figure11-12 Correct Answers -DNA polymerase
A deoxyribonucleotide is composed of:
p177 Figure 6-2 Correct Answers -Nitrogenous base
-Phosphate group
-Pentose with 3' OH
A lab is studying the role of histone proteins in DNA
compaction. Which of the following organisms could be used to
study this? Correct Answers -C. elegans
-S. cerevisiae
-M. musculus
-N. crassa
-A. thaliana
A mutant protein has been designated as G189A. What does this
designation reveal about the mutation? Correct Answers -That a
glycine at amino acid 189 of the protein is now an alanine
-That a missense mutation occurred in the gene corresponding to
amino acid 189 of the protein.
,A mutant protein has been designated as G189A. What does this
designation reveal about the mutation? Correct Answers -That a
glycine at amino acid 189 of the protein is now an alanine.
-That a missense mutation occurred in the gene corresponding to
amino acid 189 of the protein.
A plasmid vector typically has which of the following features?
p221, also Figure7-4 Correct Answers -An origin of replication.
-Small size (4-10 kB) to facilitate entry into the cell and
biochemical manipulation of the DNA.
-Unique restriction sites for insertion of DNA.
-selectable marker (like gene of antibiotic resistance) to facilitate
selection for cells containing the plasmid vector.
A polylinker is:
p219 Correct Answers -A synthetic fragment that contains
multiple sites for restriction enzymes to cut the DNA.
A recombinant DNA was constructed inserting the DNA of
interest into the AmpR gene of pBR322 vector. The vector also
contains a TetR gene which is not interrupted. After introducing
the recombinat DNA into bacterial cells, the resulting
transformants were tested on a variety of media. Which of the
following results would be expected? Correct Answers -Growth
on tetracyclin containing media.
-Death on media containing both tetracyclin and ampicillin.
A typical plasmid vector used for cloning of "eukaryotic genes
under study" contains DNA sequences specific for bacteria and
,specific for eukaryotes. Why are the bacterial components
necessary if the eukaryotic genes will be studied in eukaryotes?
Correct Answers -Because a plasmid must be
amplified/replicated in bacteria first so there is a sufficient
amount to use for experiments in eukaryotes.
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
A lab wants to identify the mutation using functional
complementation. To do so, they need to create a DNA library.
What type of library could they use? Correct Answers -Yeast
genomic library
-Yeast cDNA library
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
After transforming the plasmid library into the mutant yeast
cells, what phenotype will they observe that will allow them to
identify cells that have been complemented? Correct Answers -
Cells that can now grow at elevated temperatures.
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
Describe potential selection marker in the plasmid and media
combinations that can be utilized with this strain in the
, complementation assay. Correct Answers -The plasmid can
have a gene involved in leucine synthesis and the plate will lack
leucine.
The plasmid can have a gene involved in tryptophan synthesis
and the plate will contain tryptophan.
-The plasmid can have a gene involved in tryptophan synthesis
and the plate will lack tryptophan.
-The plasmid can have a gene of antibiotic resistance (for
example, Neomycin resistance) and the plate will be
supplemented with Neomycin antibiotic.
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (ad) and histidine (his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She fixes the mistakes from the previous experiment and now
has a complete plasmid (selection marker, origin of replication,
centromere). She then inserts telomere sequences into the
plasmid. How will this impact her transformation? Correct
Answers -It will not impact her transformation.
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (ad) and histidine (his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She starts by attempting to add the centromere DNA into a
plasmid containing the origin of replication. Unfortunately,
A complementary dNTP ("next nucleotide") forms a hydrogen
bond with an exposed base on the template DNA strand. Name
the enzyme catalyzing the bond formation between the 3' OH
group of the "preceeding nucleotide" and the 5' triphosphate of
the "next nucleotide".
p376 Figure11-12 Correct Answers -DNA polymerase
A deoxyribonucleotide is composed of:
p177 Figure 6-2 Correct Answers -Nitrogenous base
-Phosphate group
-Pentose with 3' OH
A lab is studying the role of histone proteins in DNA
compaction. Which of the following organisms could be used to
study this? Correct Answers -C. elegans
-S. cerevisiae
-M. musculus
-N. crassa
-A. thaliana
A mutant protein has been designated as G189A. What does this
designation reveal about the mutation? Correct Answers -That a
glycine at amino acid 189 of the protein is now an alanine
-That a missense mutation occurred in the gene corresponding to
amino acid 189 of the protein.
,A mutant protein has been designated as G189A. What does this
designation reveal about the mutation? Correct Answers -That a
glycine at amino acid 189 of the protein is now an alanine.
-That a missense mutation occurred in the gene corresponding to
amino acid 189 of the protein.
A plasmid vector typically has which of the following features?
p221, also Figure7-4 Correct Answers -An origin of replication.
-Small size (4-10 kB) to facilitate entry into the cell and
biochemical manipulation of the DNA.
-Unique restriction sites for insertion of DNA.
-selectable marker (like gene of antibiotic resistance) to facilitate
selection for cells containing the plasmid vector.
A polylinker is:
p219 Correct Answers -A synthetic fragment that contains
multiple sites for restriction enzymes to cut the DNA.
A recombinant DNA was constructed inserting the DNA of
interest into the AmpR gene of pBR322 vector. The vector also
contains a TetR gene which is not interrupted. After introducing
the recombinat DNA into bacterial cells, the resulting
transformants were tested on a variety of media. Which of the
following results would be expected? Correct Answers -Growth
on tetracyclin containing media.
-Death on media containing both tetracyclin and ampicillin.
A typical plasmid vector used for cloning of "eukaryotic genes
under study" contains DNA sequences specific for bacteria and
,specific for eukaryotes. Why are the bacterial components
necessary if the eukaryotic genes will be studied in eukaryotes?
Correct Answers -Because a plasmid must be
amplified/replicated in bacteria first so there is a sufficient
amount to use for experiments in eukaryotes.
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
A lab wants to identify the mutation using functional
complementation. To do so, they need to create a DNA library.
What type of library could they use? Correct Answers -Yeast
genomic library
-Yeast cDNA library
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
After transforming the plasmid library into the mutant yeast
cells, what phenotype will they observe that will allow them to
identify cells that have been complemented? Correct Answers -
Cells that can now grow at elevated temperatures.
A yeast strain is a leucine and trytophan auxotroph. It is mutated
with a chemical mutagen, resulting in cells that experience a
defect in mRNA nuclear export at elevated temperatures.
Describe potential selection marker in the plasmid and media
combinations that can be utilized with this strain in the
, complementation assay. Correct Answers -The plasmid can
have a gene involved in leucine synthesis and the plate will lack
leucine.
The plasmid can have a gene involved in tryptophan synthesis
and the plate will contain tryptophan.
-The plasmid can have a gene involved in tryptophan synthesis
and the plate will lack tryptophan.
-The plasmid can have a gene of antibiotic resistance (for
example, Neomycin resistance) and the plate will be
supplemented with Neomycin antibiotic.
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (ad) and histidine (his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She fixes the mistakes from the previous experiment and now
has a complete plasmid (selection marker, origin of replication,
centromere). She then inserts telomere sequences into the
plasmid. How will this impact her transformation? Correct
Answers -It will not impact her transformation.
Andrew Murray's sister, Andrea, is adding to her brother's work
on chromosomes. She is using cells that are unable to synthesize
adenine (ad) and histidine (his). The plasmid she is currently
working with consists of an origin of replication and the Ade
gene.
She starts by attempting to add the centromere DNA into a
plasmid containing the origin of replication. Unfortunately,
