Bio 99 Pre-Lecture Questions #1 Questions With Complete
Solutions
A deoxyribonucleotide is composed of (more than one answer)
-pentose with 3' OH
-nitrogenous base
-ribose
-phosphate group Correct Answers pentose with 3' OH,
nitrogenous base, phosphate group
An appropriate dNTP forms hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
between the 3' OH group of the 1st nucleotide and 5' phosphate
of the 2nd nucleotide. The enzyme responsible for catalyzing
formation of this bond is: Correct Answers DNA polymerase
Arthur Kornberg's group found that in vitro DNA replication
required all four dNTPs. What conclusion did they arrive at
based on this result?
-an RNA primer must also be needed for synthesis
-the DNA synthesis enzyme must be using a DNA strand as a
template for synthesis
-DNA consists of four bases
-in vitro DNA replication differs from in vivo DNA replication
Correct Answers the DNA synthesis enzyme must be using a
DNA strand as a template for synthesis
Avery found that DNA was necessary and sufficient for
bacterial transformation. Which of the following experimental
results support this statement? Select all that apply.
-removal of DNA from cell extracts prevented transformation
,-removal of RNA from cell extracts prevented transformation
-protein alone will cause transformation to occur
-DNA alone will cause transformation to occur Correct Answers
removal of DNA from cell extracts prevented transformation,
DNA alone will cause transformation to occur
Because replication take place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme: Correct Answers ligase
Bidirectional synthesis was demonstrated by an experiment
involving radiolabeled dTTP in a pulse chase experiment.
Which result told the researchers that replication is
bidirectional?
-a gradient of radioactivity, with increased density at the forks
-a gradient of radioactivity with less density at the forks
-equal amounts of radioactivity among the newly synthesized
DNA Correct Answers a gradient of radioactivity, with
increased density at the forks
Chromatin consists of Correct Answers DNA and protein
Correctly order of the enzymes used during replication?
DNA ligase, ribonuclease H, DNA polymerase, primase,
helicase Correct Answers helicase, primase, DNA polymerase,
ribonuclease H, DNA ligase
, DNA partially unwinds as the hydrogen bonds between
complementary bases are broken. The enzyme responsible for
this is: Correct Answers DNA helicase
DNA polymerases also have nuclease activities. Which of the
following is not a use for the nuclease activity of DNA
polymerase I?
-proofreading during synthesis
-trimming single-stranded ends
-removal of RNA primers
-degradation of viral DNAs
-removal of DNA lesions during repair Correct Answers
degradation of viral DNAs
DNA/RNA is the less stable molecule because:
-RNA, because it is single stranded
-DNA, because it has a 2' H group
-DNA, because it is double stranded
-RNA, because it has a 2' OH group
-Both molecules are equally stable Correct Answers RNA,
because it has a 2' OH group
Each nucleosome contains Correct Answers a mixture of
histones and SMC proteins
Genomics is the field dedicated to the study of:
-DNA on a cellular scale
-protein expression
-RNA synthesis and function
Solutions
A deoxyribonucleotide is composed of (more than one answer)
-pentose with 3' OH
-nitrogenous base
-ribose
-phosphate group Correct Answers pentose with 3' OH,
nitrogenous base, phosphate group
An appropriate dNTP forms hydrogen bond with an exposed
base on the template strand. The next dNTP forms a bond
between the 3' OH group of the 1st nucleotide and 5' phosphate
of the 2nd nucleotide. The enzyme responsible for catalyzing
formation of this bond is: Correct Answers DNA polymerase
Arthur Kornberg's group found that in vitro DNA replication
required all four dNTPs. What conclusion did they arrive at
based on this result?
-an RNA primer must also be needed for synthesis
-the DNA synthesis enzyme must be using a DNA strand as a
template for synthesis
-DNA consists of four bases
-in vitro DNA replication differs from in vivo DNA replication
Correct Answers the DNA synthesis enzyme must be using a
DNA strand as a template for synthesis
Avery found that DNA was necessary and sufficient for
bacterial transformation. Which of the following experimental
results support this statement? Select all that apply.
-removal of DNA from cell extracts prevented transformation
,-removal of RNA from cell extracts prevented transformation
-protein alone will cause transformation to occur
-DNA alone will cause transformation to occur Correct Answers
removal of DNA from cell extracts prevented transformation,
DNA alone will cause transformation to occur
Because replication take place in the 5' to 3' direction only, the
lagging strand consists of short 5' to 3' segments called Okazaki
fragments. The Okazaki fragments are joined together by the
enzyme: Correct Answers ligase
Bidirectional synthesis was demonstrated by an experiment
involving radiolabeled dTTP in a pulse chase experiment.
Which result told the researchers that replication is
bidirectional?
-a gradient of radioactivity, with increased density at the forks
-a gradient of radioactivity with less density at the forks
-equal amounts of radioactivity among the newly synthesized
DNA Correct Answers a gradient of radioactivity, with
increased density at the forks
Chromatin consists of Correct Answers DNA and protein
Correctly order of the enzymes used during replication?
DNA ligase, ribonuclease H, DNA polymerase, primase,
helicase Correct Answers helicase, primase, DNA polymerase,
ribonuclease H, DNA ligase
, DNA partially unwinds as the hydrogen bonds between
complementary bases are broken. The enzyme responsible for
this is: Correct Answers DNA helicase
DNA polymerases also have nuclease activities. Which of the
following is not a use for the nuclease activity of DNA
polymerase I?
-proofreading during synthesis
-trimming single-stranded ends
-removal of RNA primers
-degradation of viral DNAs
-removal of DNA lesions during repair Correct Answers
degradation of viral DNAs
DNA/RNA is the less stable molecule because:
-RNA, because it is single stranded
-DNA, because it has a 2' H group
-DNA, because it is double stranded
-RNA, because it has a 2' OH group
-Both molecules are equally stable Correct Answers RNA,
because it has a 2' OH group
Each nucleosome contains Correct Answers a mixture of
histones and SMC proteins
Genomics is the field dedicated to the study of:
-DNA on a cellular scale
-protein expression
-RNA synthesis and function
