"DNA footprinting" is a technique that can be used to identify
Correct Answers the specific binding site of a repressor,
polymerase, or other protein on the DNA
A eukaryotic ribosome stalled by a non-stop mutation can be
rescued by: Correct Answers non-stop mRNA decay.
A function of which initiation factor is to prevent premature
joining of the large and small ribosomal subunits? Correct
Answers eIF3 in eukaryotes and IF-3 in bacteria
A housekeeping gene is one that codes for a product that Correct
Answers is expressed at a constitutive level
A transcription factor that enhances gene expression is called a
Correct Answers activator
After determining that the genetic code is read in triplet codons,
the question arose as to whether the code was overlapping or
nonoverlapping. If the code was overlapping, a change in one
nucleotide could result in a change in how many amino acids?
Correct Answers three
Because the genetic code is nonoverlapping, a missense
mutation (from a single nucleotide change) results in the
alteration of ______________, and the resulting protein has
______________. Correct Answers only one codon / a single
amino acid change
, Chromatin is composed of Correct Answers DNA and protein
Each nucleotide contains Correct Answers a histone octamer
composed of 2 copies of each of the following proteins: H2A,
H2B, H3, and H4
Eukaryotic proteins do not require a targeting signal if they are
destined to function in the: Correct Answers cytoplasm.
How can repositioning nucleosomes affect transcription?
Correct Answers - Moving nucleosomes could cover a promoter
resulting in repression of transcription.
- Moving nucleosomes could expose a promoter resulting in
activation of transcription.
Insulators Correct Answers - are boundary elements that
function to prevent the enhancer for one gene from activating
the promoter of another gene
- are DNA sequences that can prevent packaging of a gene into
heterochromatin
- require the zinc-finger protein CTCF to function in higher
eukaryotes
Steroid hormone-nuclear receptor complexes activate
transcription by binding to Correct Answers hormone response
elements
The "charging" or activation of a tRNA molecule includes:
Correct Answers the covalent attachment of the carboxyl group
of an amino acid to the tRNA.