AAB immunology Knowledge test Exam Toolkit Questions
and Verified solution latest 2025/2026
Needed for lysis invitro, complement fixation - correct answer antigen (
bact/rbc)+antibody ( bacteriolysin or hemolysin)+ complement
In vivo complement dependent antibody lysis: rbc - correct answer direct
lysis: after blood transfusion
Inc phacogytosis in spleen of rbc coated with antibody/complement ( bacterial
infec, viral
In vivo complement dependent antibody lysis: plaelet - correct answer
antibody sensitization in ITP or antidrug antibodies
In vivo complement dependent antibody lysis: PMN - correct answer
antibody or antidrug antibody sensitization
In vivo complement dependent antibody lysis:
Lymphocytes - correct answer antibodies in SLE that specifically lyse T cells
In vivo complement dependent antibody lysis: specific cells in certain tissues -
correct answer antibodies to glom or pulm basement membrane in
Goodpasture's disease
Opsonins - correct answer may be specific antibody
Igg1, 3
Spec antibody+ complement via classical complement pathway
,Complement alone via alt pathway
Pyogenic microorganisms ( extracellular pathogens) posses antiphagocytic
surface factors, over come by opsonins)
Monoclonal antibodies are made from - correct answer hybridomas
Hybridomas are - correct answer cell culture consisting of a clone of fused
( hybrid) cells of different kinds
B cells of an immunized animals+ tumor cell ) plasma myeloma
Wont cross link to form precipitate
- dont use for hemagglutination or precipitation
Cant modify avidity- binds to single epitope
Doesnt fix complement
Monoclonal antibody assays - correct answer immunoradiometric assay
( IRMA)
EIA
IF
IRMA or EIA procedures - correct answer solid phase antibody ( bound to
solid phase) spec for one epitope, seodn enzyme or radio labled antibody
specific for a different epitope can react with antigen in a single step
,Elim the 2 step sequential additon of antigen and labled antibody to solid
phase, avoiding one incub step and a wash
Affinity - correct answer strength of single binding site
Avidity - correct answer sum of strengths of all binding sites
Presence of recent or current disease - correct answer sera collected with a
time period intervening
- ab present in second specimen but not first
- amount of ab ( igm) found in second specimen significantly higher ( 4x rise)
than in first
Gel immunodiffusion - correct answer detect ab-ag complex formed by
precipitation,
Random movement, semisolid medium
Dependent on: buffer, ph, temp, particle size, gel viscosity, hydration
Gel immunodiffusion: single diffusion - correct answer one reactant moving,
single dimension ( up or down)
Also with
Double dimension ( moving out radially from well)
, Gel immunodiffusion: double diffusion - correct answer both reactants
moving, single dimension
Also
Double dimension
One dimension diffusion - correct answer gel or antibody remain fixed and
other reactant moves and complexes with it
Oudin- linear diffusion
(one dimension diffusion) - correct answer antibody in agar tube
Antigen overlay, antigen moves through the gel to for precipitin band at slight
antigen xs
Detects mult ag-ab interaction
Single radial diffusion
(one dimension diffusion) - correct answer spec Ab in agar plate
Cut antigen into well, should diffuse
Pos= ring will form indicate binding
Mancini- ring= porportion to antigen concen after 48 hrs
Fahey- diameter of ring= log of antigen concen after 24 hr diffusion
and Verified solution latest 2025/2026
Needed for lysis invitro, complement fixation - correct answer antigen (
bact/rbc)+antibody ( bacteriolysin or hemolysin)+ complement
In vivo complement dependent antibody lysis: rbc - correct answer direct
lysis: after blood transfusion
Inc phacogytosis in spleen of rbc coated with antibody/complement ( bacterial
infec, viral
In vivo complement dependent antibody lysis: plaelet - correct answer
antibody sensitization in ITP or antidrug antibodies
In vivo complement dependent antibody lysis: PMN - correct answer
antibody or antidrug antibody sensitization
In vivo complement dependent antibody lysis:
Lymphocytes - correct answer antibodies in SLE that specifically lyse T cells
In vivo complement dependent antibody lysis: specific cells in certain tissues -
correct answer antibodies to glom or pulm basement membrane in
Goodpasture's disease
Opsonins - correct answer may be specific antibody
Igg1, 3
Spec antibody+ complement via classical complement pathway
,Complement alone via alt pathway
Pyogenic microorganisms ( extracellular pathogens) posses antiphagocytic
surface factors, over come by opsonins)
Monoclonal antibodies are made from - correct answer hybridomas
Hybridomas are - correct answer cell culture consisting of a clone of fused
( hybrid) cells of different kinds
B cells of an immunized animals+ tumor cell ) plasma myeloma
Wont cross link to form precipitate
- dont use for hemagglutination or precipitation
Cant modify avidity- binds to single epitope
Doesnt fix complement
Monoclonal antibody assays - correct answer immunoradiometric assay
( IRMA)
EIA
IF
IRMA or EIA procedures - correct answer solid phase antibody ( bound to
solid phase) spec for one epitope, seodn enzyme or radio labled antibody
specific for a different epitope can react with antigen in a single step
,Elim the 2 step sequential additon of antigen and labled antibody to solid
phase, avoiding one incub step and a wash
Affinity - correct answer strength of single binding site
Avidity - correct answer sum of strengths of all binding sites
Presence of recent or current disease - correct answer sera collected with a
time period intervening
- ab present in second specimen but not first
- amount of ab ( igm) found in second specimen significantly higher ( 4x rise)
than in first
Gel immunodiffusion - correct answer detect ab-ag complex formed by
precipitation,
Random movement, semisolid medium
Dependent on: buffer, ph, temp, particle size, gel viscosity, hydration
Gel immunodiffusion: single diffusion - correct answer one reactant moving,
single dimension ( up or down)
Also with
Double dimension ( moving out radially from well)
, Gel immunodiffusion: double diffusion - correct answer both reactants
moving, single dimension
Also
Double dimension
One dimension diffusion - correct answer gel or antibody remain fixed and
other reactant moves and complexes with it
Oudin- linear diffusion
(one dimension diffusion) - correct answer antibody in agar tube
Antigen overlay, antigen moves through the gel to for precipitin band at slight
antigen xs
Detects mult ag-ab interaction
Single radial diffusion
(one dimension diffusion) - correct answer spec Ab in agar plate
Cut antigen into well, should diffuse
Pos= ring will form indicate binding
Mancini- ring= porportion to antigen concen after 48 hrs
Fahey- diameter of ring= log of antigen concen after 24 hr diffusion
