AAB Molecular Diagnostics Testing Test Exam Review
Questions with all Answers verified for accuracy latest
2025/2026
RNase H cleaves the RNA from the middle of the probe in what amplification
technique? - correct answer Cycling Probe
How is the reporter dye and quencher dye separated in the Cycling Probe
test? - correct answer When the probe is digested.
The Hybrid Capture Assay resembles with chemistry/EIA technique? - correct
answer Sandwich Assay
Primer dimers result from? - correct answer 3' complementarity in the
sequence primer
What control is ran to detect contamination in a PCR test? - correct answer
Reagent Blank
Nonspecific extra PCR products can result from? - correct answer
Mispriming
How many copies of a target is made after 20 cycles of PCR? - correct
answer 2^20
What are the three basic steps in a PCR cycle? - correct answer
Denaturation, annealing, and extension
, Real-time PCR is considered to be a Qualitative or Quantitative test? -
correct answer Quantitative
This DNA amplification method amplifies the nucleic acid used as a probe? -
correct answer Ligase Chain Reaction (LCR)
What enzymes are used in Ligase Chain Reactions? - correct answer DNA
polymerase and DNA ligase
What is the function of DNA polymerase in Ligase Chain Reaction? - correct
answer Is used to initiate DNA polymerase.
What is the key advantage of using Ligase Chain Reaction instead of
Polymerase Chain Reaction? - correct answer Better specificity
How does Ligase Chain Reaction differ from Polymerase Chain Reaction? -
correct answer This method uses a thermostable ligase to join to probes
which are then amplified by standard PCR cycling.
Why was Ligase Chain Reaction originally developed? - correct answer To
detect point mutation.
What do positive controls ensure during PCR testing? - correct answer That
the enzyme is active, the buffer is optimal, the primers are priming the correct
sequence, and that the thermal cycler is working properly.
What is a reagent blank control in PCR? - correct answer A negative control
that lacks DNA to ensure that the reaction mix is not contaminated with DNA
template or amplified products from a previous run.
Questions with all Answers verified for accuracy latest
2025/2026
RNase H cleaves the RNA from the middle of the probe in what amplification
technique? - correct answer Cycling Probe
How is the reporter dye and quencher dye separated in the Cycling Probe
test? - correct answer When the probe is digested.
The Hybrid Capture Assay resembles with chemistry/EIA technique? - correct
answer Sandwich Assay
Primer dimers result from? - correct answer 3' complementarity in the
sequence primer
What control is ran to detect contamination in a PCR test? - correct answer
Reagent Blank
Nonspecific extra PCR products can result from? - correct answer
Mispriming
How many copies of a target is made after 20 cycles of PCR? - correct
answer 2^20
What are the three basic steps in a PCR cycle? - correct answer
Denaturation, annealing, and extension
, Real-time PCR is considered to be a Qualitative or Quantitative test? -
correct answer Quantitative
This DNA amplification method amplifies the nucleic acid used as a probe? -
correct answer Ligase Chain Reaction (LCR)
What enzymes are used in Ligase Chain Reactions? - correct answer DNA
polymerase and DNA ligase
What is the function of DNA polymerase in Ligase Chain Reaction? - correct
answer Is used to initiate DNA polymerase.
What is the key advantage of using Ligase Chain Reaction instead of
Polymerase Chain Reaction? - correct answer Better specificity
How does Ligase Chain Reaction differ from Polymerase Chain Reaction? -
correct answer This method uses a thermostable ligase to join to probes
which are then amplified by standard PCR cycling.
Why was Ligase Chain Reaction originally developed? - correct answer To
detect point mutation.
What do positive controls ensure during PCR testing? - correct answer That
the enzyme is active, the buffer is optimal, the primers are priming the correct
sequence, and that the thermal cycler is working properly.
What is a reagent blank control in PCR? - correct answer A negative control
that lacks DNA to ensure that the reaction mix is not contaminated with DNA
template or amplified products from a previous run.
