Molecular Diagnostics Chapter 1 Study
Questions with 100% Correct Answers |
Expert Graded A+ (Guarantee Pass)
1. In a DNA molecule, the 5' end contains a free _________________ group. -
ANSWER phosphate
2. In a DNA molecule, the 3' end contains a _________________ end -
ANSWER hydroxyl
3. In a DNA molecule, one end has a free hydroxyl group and one end has a
free what? - ANSWER phosphate group
4. The nucleotides in single strand of DNA are held together by
_________________ bonds between sugar and phosphate groups -
ANSWER phosphodiester
5. In DNA replication, where does replication begin and end?
A. origin of replication end of the molecule
B. promoter; termination site
C. start codon; stop codon
D. start codon; termination site - ANSWER origin or replication; end of
molecule
6. Which of the following enzymes will untangle DNA?
A. polymerase
B. Helicase
C. kinase
D. phosphatase - ANSWER Helicase
,7. Enzymes that recognize palindromic sequences of DNA, that are cut within
the recognition sequence, that do not have methylating activity, and that are
used frequently in the laboratory are which type of restriction enzymes?
A. Type I
B. Type II
C. Type III
D. Type IV - ANSWER Type II
8. Which of the following is a type II restriction enzyme recognition site?
A. GAATTC
B. GAATTG
C. GAAAAG
D. GATCAG - ANSWER GAATTC
9. Restriction endonucleases are enzymes that are produced by bacteria
and__________________
A. degrade viral proteins
B. digest DNA
C. have no laboratory applications
D. degrade lipids - ANSWER digest DNA
10.The function of endonucleases is to: - ANSWER cut DNA within the
double helix
11.Which type of restriction enzyme has both nuclease and methylase in a
single enzyme and bind to host specific DNA sites of 4 to 6 bp and contain
methylated adenines
A. Type I
B. Type II
C. Type III
D. Type VI - ANSWER Type I
,12.Example of Type I restriction enzyme:` - ANSWER EcoK from E. coli K
12
13.Restriction endonuclease are enzymes that are produced by bacteria and
what else? - ANSWER Archae
14.In sexual recombination, new combinations of genes are created by which:
A. transduction
B. crossing over
C. conjugation
D. transformation - ANSWER crossing over
15.Recombination between a plasmid and the cel chromosome will result in:
A. Rapid degradation of the chromosome
B. death of the cell
C. immediate cellular replication
D. stable integration - ANSWER Stable integration
16.Nitrogen bases with a double ring structure (guanine, adenine) are: -
ANSWER purines
17.What type of bonds spontaneously form between two complementary
strands of DNA?
A. hydrogen bonds
B. covalent bods
C. phosphodiester bonds
D. polar covalent bonds - ANSWER hydrogen bonds
18.In a hydrogen bond, GUANINES in one chain form THREE hydrogen
bonds with ________________ in the opposite chain. - ANSWER cytosines
, 19.In a hydrogen bond, adenines form TWO hydrogen bonds with what in the
opposite chain: - ANSWER thymines
20.In DNA replication, the leading strand is the strand that has which
orientation? - ANSWER 3' to 5'
21.In DNA replication, the leading strand is the strand that proceeds in which
manner? - ANSWER continuous
22.Which of the following types of RNA is directly involved in removing
introns from RNA in eukaryotes
A. micro
B. transfer
C. small nuclear
D. small interfering - ANSWER Small nuclear
23.The loop of transfer RNA that interacts with the codon on mRNA in
translation is called:
A. D loop
B. TUC loop
C. variable loop
D. anticodon loop - ANSWER anticodon loop
24.In transcription, what is the starting material, the ending material, and the
major enzyme that catalyzes the process?
A. DNA, RNA, DNA polymerase
B. RNA, protein, peptide transferase
C. RNA, DNA reverse transcriptase
D. DNA, RNA, RNA polymerase - ANSWER DNA, RNA, RNA
polymerase
Questions with 100% Correct Answers |
Expert Graded A+ (Guarantee Pass)
1. In a DNA molecule, the 5' end contains a free _________________ group. -
ANSWER phosphate
2. In a DNA molecule, the 3' end contains a _________________ end -
ANSWER hydroxyl
3. In a DNA molecule, one end has a free hydroxyl group and one end has a
free what? - ANSWER phosphate group
4. The nucleotides in single strand of DNA are held together by
_________________ bonds between sugar and phosphate groups -
ANSWER phosphodiester
5. In DNA replication, where does replication begin and end?
A. origin of replication end of the molecule
B. promoter; termination site
C. start codon; stop codon
D. start codon; termination site - ANSWER origin or replication; end of
molecule
6. Which of the following enzymes will untangle DNA?
A. polymerase
B. Helicase
C. kinase
D. phosphatase - ANSWER Helicase
,7. Enzymes that recognize palindromic sequences of DNA, that are cut within
the recognition sequence, that do not have methylating activity, and that are
used frequently in the laboratory are which type of restriction enzymes?
A. Type I
B. Type II
C. Type III
D. Type IV - ANSWER Type II
8. Which of the following is a type II restriction enzyme recognition site?
A. GAATTC
B. GAATTG
C. GAAAAG
D. GATCAG - ANSWER GAATTC
9. Restriction endonucleases are enzymes that are produced by bacteria
and__________________
A. degrade viral proteins
B. digest DNA
C. have no laboratory applications
D. degrade lipids - ANSWER digest DNA
10.The function of endonucleases is to: - ANSWER cut DNA within the
double helix
11.Which type of restriction enzyme has both nuclease and methylase in a
single enzyme and bind to host specific DNA sites of 4 to 6 bp and contain
methylated adenines
A. Type I
B. Type II
C. Type III
D. Type VI - ANSWER Type I
,12.Example of Type I restriction enzyme:` - ANSWER EcoK from E. coli K
12
13.Restriction endonuclease are enzymes that are produced by bacteria and
what else? - ANSWER Archae
14.In sexual recombination, new combinations of genes are created by which:
A. transduction
B. crossing over
C. conjugation
D. transformation - ANSWER crossing over
15.Recombination between a plasmid and the cel chromosome will result in:
A. Rapid degradation of the chromosome
B. death of the cell
C. immediate cellular replication
D. stable integration - ANSWER Stable integration
16.Nitrogen bases with a double ring structure (guanine, adenine) are: -
ANSWER purines
17.What type of bonds spontaneously form between two complementary
strands of DNA?
A. hydrogen bonds
B. covalent bods
C. phosphodiester bonds
D. polar covalent bonds - ANSWER hydrogen bonds
18.In a hydrogen bond, GUANINES in one chain form THREE hydrogen
bonds with ________________ in the opposite chain. - ANSWER cytosines
, 19.In a hydrogen bond, adenines form TWO hydrogen bonds with what in the
opposite chain: - ANSWER thymines
20.In DNA replication, the leading strand is the strand that has which
orientation? - ANSWER 3' to 5'
21.In DNA replication, the leading strand is the strand that proceeds in which
manner? - ANSWER continuous
22.Which of the following types of RNA is directly involved in removing
introns from RNA in eukaryotes
A. micro
B. transfer
C. small nuclear
D. small interfering - ANSWER Small nuclear
23.The loop of transfer RNA that interacts with the codon on mRNA in
translation is called:
A. D loop
B. TUC loop
C. variable loop
D. anticodon loop - ANSWER anticodon loop
24.In transcription, what is the starting material, the ending material, and the
major enzyme that catalyzes the process?
A. DNA, RNA, DNA polymerase
B. RNA, protein, peptide transferase
C. RNA, DNA reverse transcriptase
D. DNA, RNA, RNA polymerase - ANSWER DNA, RNA, RNA
polymerase
