BLOOD BANK - SEROLOGIC AND
MOLECULAR TESTING QUESTIONS AND
ANSWERS WITH CORRECT SOLUTIONS
UPDATED VERSION
Which adsorption technique removes cold (IgM)
antibodies, particularly anti-I specificities?
A. Cold autoadsorption
B. Warm autoadsorption
C. Differential (allogeneic)
D. Rabbit erythrocyte stroma (RESt) - CORRECT
ANSWES -- D
At what frequency should quality control testing be
performed on each lot of anti-human globulin to be
in compliance with the FDA's current good
manufacturing practices requirements?
A. At the beginning of each work shift
B. Each day of use
,C. Only at the time of receipt
D. By each new user - CORRECT ANSWES -- B
A patient with multiple myeloma has the following
reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - CORRECT
ANSWES -- A
,An antibody screen utilizing polyethylene glycol (PEG)
was performed. Reaction readings of this antibody
screen are analyzed after immediate spin and again
at 37°C incubation with subsequent centrifugation.
How will the centrifugation of the tubes affect the
patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - CORRECT ANSWES --
C
What must be true for the antiglobulin phase of the
serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is
not history of detection of unexpected antibodies.
, B. The patient has not been transfused within the past
24 hours.
C. The blood is needed for surgery.
D. There is a history of detection of unexpected
antibodies but the current antibody screen is
negative. - CORRECT ANSWES -- A
Which of the following accurately represents reverse
typing?
A. Reverse typing is performed using known reagent
antisera to detect ABO antigens on the patient's red
blood cells.
B. Reverse typing is performed using known reagent
red blood cells to detect ABO antibodies in the
patient's serum or plasma.
C. Reverse typing is performed using known reagent
screening cells to detect clinically significant
antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent
antisera to phenotype donor red blood cells for
MOLECULAR TESTING QUESTIONS AND
ANSWERS WITH CORRECT SOLUTIONS
UPDATED VERSION
Which adsorption technique removes cold (IgM)
antibodies, particularly anti-I specificities?
A. Cold autoadsorption
B. Warm autoadsorption
C. Differential (allogeneic)
D. Rabbit erythrocyte stroma (RESt) - CORRECT
ANSWES -- D
At what frequency should quality control testing be
performed on each lot of anti-human globulin to be
in compliance with the FDA's current good
manufacturing practices requirements?
A. At the beginning of each work shift
B. Each day of use
,C. Only at the time of receipt
D. By each new user - CORRECT ANSWES -- B
A patient with multiple myeloma has the following
reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - CORRECT
ANSWES -- A
,An antibody screen utilizing polyethylene glycol (PEG)
was performed. Reaction readings of this antibody
screen are analyzed after immediate spin and again
at 37°C incubation with subsequent centrifugation.
How will the centrifugation of the tubes affect the
patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - CORRECT ANSWES --
C
What must be true for the antiglobulin phase of the
serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is
not history of detection of unexpected antibodies.
, B. The patient has not been transfused within the past
24 hours.
C. The blood is needed for surgery.
D. There is a history of detection of unexpected
antibodies but the current antibody screen is
negative. - CORRECT ANSWES -- A
Which of the following accurately represents reverse
typing?
A. Reverse typing is performed using known reagent
antisera to detect ABO antigens on the patient's red
blood cells.
B. Reverse typing is performed using known reagent
red blood cells to detect ABO antibodies in the
patient's serum or plasma.
C. Reverse typing is performed using known reagent
screening cells to detect clinically significant
antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent
antisera to phenotype donor red blood cells for
