3/5/26, 7:48
PM
GMU MICRO BIO 306 FINAL EXAM WITH VERIFIED QUESTIONS AND
ANSWERS 2026
You have a 5M stock solution and (a) C1V1 = C2V2
want to dilute it down to a 0.2M (5)V1 = (0.2)(25mL)
solution that has a final volume of
V1 = 1mL
25mL. (a) how much stock solution
would you need to add? (b) how much
diluent do you need to add to the (b) 25 - 1mL = 24mL
solution to bring the total volume up
to 25 mL?
Explain the difference between - chemically defined is composed of chemicals
chemically defined media and -complex is composed of many components
complex media (potentially chemicals in addition to other
substances)
A gram positive stained organism is (a) purple / peptidoglycan layer
what color, and is stained that color
due to?
(a) purple / peptidoglycan layer
(b) purple / lipopolysaccharide layer (LPS)
(c) pink / peptidoglycan layer
(d) pink / lipopolysaccharide layer (LPS)
What would be a simple way to Plate the mixed culture (streak plate)
separate two different bacterial strains
from a broth solution?
If you want to make a 40% NaCl g/mL = __%
solution how many grams of NaCl g/200mL =
would you add to 200mL? 40%
g = 80g
True or false: bacterial culture media true
must be sterilized prior to use
Which class of organisms would (b) beta hemolytic
completely lyse red blood cells and
leave a completely clear zone
around itself in a blood agar plate?
(a) alpha hemolytic
(b) beta hemolytic
(c) gamma hemolytic
Define selective media and differential - selective media: only certain bacteria can grow on it
media -differential media: bacteria shows distinction by
color changes or chemical changes
What does it mean to have a "pure "pure culture" means the culture contains one bacterium
culture"? Name one way you can test that has not been
to see if your culture is pure. contaminated with an outside bacteria. By completing a
streak plate, one can determine if a culture is pure
based on if there is only one type of colony
growing on the agar or not
https://quizlet.com/110014709/gmu-micro-bio-306-final- 1/
flash-cards/ 3
PM
GMU MICRO BIO 306 FINAL EXAM WITH VERIFIED QUESTIONS AND
ANSWERS 2026
You have a 5M stock solution and (a) C1V1 = C2V2
want to dilute it down to a 0.2M (5)V1 = (0.2)(25mL)
solution that has a final volume of
V1 = 1mL
25mL. (a) how much stock solution
would you need to add? (b) how much
diluent do you need to add to the (b) 25 - 1mL = 24mL
solution to bring the total volume up
to 25 mL?
Explain the difference between - chemically defined is composed of chemicals
chemically defined media and -complex is composed of many components
complex media (potentially chemicals in addition to other
substances)
A gram positive stained organism is (a) purple / peptidoglycan layer
what color, and is stained that color
due to?
(a) purple / peptidoglycan layer
(b) purple / lipopolysaccharide layer (LPS)
(c) pink / peptidoglycan layer
(d) pink / lipopolysaccharide layer (LPS)
What would be a simple way to Plate the mixed culture (streak plate)
separate two different bacterial strains
from a broth solution?
If you want to make a 40% NaCl g/mL = __%
solution how many grams of NaCl g/200mL =
would you add to 200mL? 40%
g = 80g
True or false: bacterial culture media true
must be sterilized prior to use
Which class of organisms would (b) beta hemolytic
completely lyse red blood cells and
leave a completely clear zone
around itself in a blood agar plate?
(a) alpha hemolytic
(b) beta hemolytic
(c) gamma hemolytic
Define selective media and differential - selective media: only certain bacteria can grow on it
media -differential media: bacteria shows distinction by
color changes or chemical changes
What does it mean to have a "pure "pure culture" means the culture contains one bacterium
culture"? Name one way you can test that has not been
to see if your culture is pure. contaminated with an outside bacteria. By completing a
streak plate, one can determine if a culture is pure
based on if there is only one type of colony
growing on the agar or not
https://quizlet.com/110014709/gmu-micro-bio-306-final- 1/
flash-cards/ 3
