AORN Perioperative 101 – Anesthesia, Surgical Safety & Sterile Technique | 150 Exam Questions with Verified Answers | Chamberlain University 2026

This document contains 150 AORN Perioperative 101 exam practice questions with verified answers designed to support nursing students and perioperative trainees preparing for operating room competency exams and perioperative certification assessments. The content is structured in a clear question-and-answer study format that reinforces essential clinical knowledge related to anesthesia principles, sterile technique, surgical safety, infection prevention, patient positioning, and perioperative nursing responsibilities. The study material allows learners to review high-yield perioperative concepts frequently assessed in AORN Periop 101 courses and hospital-based surgical nursing training programs. The questions review a broad range of perioperative topics including types of anesthesia (general, regional, local, and monitored anesthesia care), the four stages and three phases of anesthesia, malignant hyperthermia recognition and treatment, surgical fire prevention, and patient monitoring during anesthesia care. Additional sections explore infection prevention strategies, surgical hand antisepsis, sterile field management, electrosurgical unit safety, specimen handling procedures, wound classification systems, and perioperative documentation requirements. For example, early sections explain anesthesia provider roles and stages of anesthesia, while later sections discuss sterile technique, surgical instrument handling, patient positioning risks, wound healing phases, and surgical site infection prevention (pages 1–20). The content aligns closely with the Association of periOperative Registered Nurses (AORN) Periop 101: A Core Curriculum™, which is widely used to train nurses transitioning into perioperative and operating room practice. The document also complements major perioperative nursing textbooks such as “Alexander’s Care of the Patient in Surgery” and “Berry & Kohn’s Operating Room Technique,” both of which provide foundational knowledge for surgical nursing practice, sterile processing standards, perioperative patient safety, and operating room workflow management. The questions in this study guide reinforce clinical reasoning and procedural knowledge necessary for safe surgical patient care. This document may be beneficial for students enrolled in perioperative nursing, surgical nursing, operating room orientation programs, and surgical technology training courses. It is especially relevant for learners preparing for AORN Periop 101 examinations, perioperative clinical competency assessments, or CNOR certification preparation. The material may support students studying in RN perioperative residency programs, transition-to-operating-room training programs, perioperative clinical practicums, and advanced nursing clinical skills courses. It may also be useful for nursing students and practicing nurses from institutions such as Chamberlain University, Walden University, Purdue University Global, Herzing University, Rasmussen University, and other nursing schools offering perioperative or surgical nursing education. Keywords: AORN perioperative 101 exam questions, perioperative nursing exam preparation, operating room nursing practice questions, anesthesia stages and phases nursing review, malignant hyperthermia management nursing, sterile technique operating room guidelines, surgical fire prevention nursing review, perioperative infection prevention practices, surgical wound classification nursing exam, perioperative patient positioning safety, electrosurgical unit safety guidelines, surgical specimen handling procedures nursing, perioperative documentation standards review, surgical hand antisepsis guidelines nursing, wound healing phases perioperative nursing, surgical count safety procedures, perioperative nursing competency exam preparation, CNOR certification practice questions, operating room safety protocols nursing, perioperative patient assessment review