Bioprocess Engineering Principles — Solutions
Manual (All 14 Chapters)
bioprocess engineering - answer-bioprocess engineering is the sub-discipline within
biotechnology that is responsible for translating the discoveries of life science into practical
products, processes, or systems that can serve the needs of society
Chemical engineer - answer-chemistry, physics, math, economics, biology
Biology is - answer-a vast field
In addition to learning engineering calculations - answer-you will need to learn fundamental
concepts of biology consisting of qualitative information
Bioprocessing industry serves many sectors: - answer-pharmaceuticals, bio-pharmaceuticals,
food, animal feed, biofuels, biobased chemicals, waste treatment, petroleum production
Industrial bioproducts - answer-organic acids, alcohols, amino acids, enzymes, bioplastics,
antibiotics, biopharmaceuticals, nutraceuticals
In 1928, ________ _______ observed ______ ________ ______ on a petri dish near
contaminating organism _________ _______. - answer-Alexander Fleming, no bacterial growth,
Penicillium notatum
_______ ________ and _______ _______ prepared a stable form of penicillin from ________
________ that can be grown in _________ _______ and produced ________ times the amount
of Penicillium notatum. - answer-Howard Florey, Ernest Chain, Penicillium chrysogenum,
1
, submerged culture, 200
What were the challenges of commercial production? - answer-Low rate of production, low
concentration (~0.001 g/L), low oxygen transfer rate, product instability
Developments - answer-Corn steep liquor, better strain from moldy cantaloupe, "surface
method"
Corn steep liquor - answer-Byproduct of corn wet-milling, adding CSL made penicillin production
increase x10
Penicillium chrysogenum - answer-Came from moldy cantaloupe at a fruit market, strain
produced much more penicillin than original strain
Surface method - answer-Mold grows on the surface of liquid in bottles, allowed for more
oxygen coming from air, high yield but labor intensive
Submerged tank process - answer-Mold grown fully submerged in liquid inside large stirred
fermentation tanks, increased product concentration from 0.001 g/L to over 50 g/L
The commercialization - answer-14 fermenters with 7000 gallons each allowed for treating
100,000 patients per year by end of WWII
Major types of biomolecules - answer-carbohydrates, lipids, proteins, nucleic acids
Composition of cells - answer-70% H2O
30% chemicals:
2
Manual (All 14 Chapters)
bioprocess engineering - answer-bioprocess engineering is the sub-discipline within
biotechnology that is responsible for translating the discoveries of life science into practical
products, processes, or systems that can serve the needs of society
Chemical engineer - answer-chemistry, physics, math, economics, biology
Biology is - answer-a vast field
In addition to learning engineering calculations - answer-you will need to learn fundamental
concepts of biology consisting of qualitative information
Bioprocessing industry serves many sectors: - answer-pharmaceuticals, bio-pharmaceuticals,
food, animal feed, biofuels, biobased chemicals, waste treatment, petroleum production
Industrial bioproducts - answer-organic acids, alcohols, amino acids, enzymes, bioplastics,
antibiotics, biopharmaceuticals, nutraceuticals
In 1928, ________ _______ observed ______ ________ ______ on a petri dish near
contaminating organism _________ _______. - answer-Alexander Fleming, no bacterial growth,
Penicillium notatum
_______ ________ and _______ _______ prepared a stable form of penicillin from ________
________ that can be grown in _________ _______ and produced ________ times the amount
of Penicillium notatum. - answer-Howard Florey, Ernest Chain, Penicillium chrysogenum,
1
, submerged culture, 200
What were the challenges of commercial production? - answer-Low rate of production, low
concentration (~0.001 g/L), low oxygen transfer rate, product instability
Developments - answer-Corn steep liquor, better strain from moldy cantaloupe, "surface
method"
Corn steep liquor - answer-Byproduct of corn wet-milling, adding CSL made penicillin production
increase x10
Penicillium chrysogenum - answer-Came from moldy cantaloupe at a fruit market, strain
produced much more penicillin than original strain
Surface method - answer-Mold grows on the surface of liquid in bottles, allowed for more
oxygen coming from air, high yield but labor intensive
Submerged tank process - answer-Mold grown fully submerged in liquid inside large stirred
fermentation tanks, increased product concentration from 0.001 g/L to over 50 g/L
The commercialization - answer-14 fermenters with 7000 gallons each allowed for treating
100,000 patients per year by end of WWII
Major types of biomolecules - answer-carbohydrates, lipids, proteins, nucleic acids
Composition of cells - answer-70% H2O
30% chemicals:
2
