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HESI A2 Biology: 100+ Exam Questions and Verified Solutions (2025/2026 Edition)

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Ace your HESI A2 exam with this comprehensive biology question bank, featuring detailed answers and explanations . This guide covers essential Chemistry of Life topics, including water polarity, hydrogen bonding, and the structure and function of biological molecules like proteins, carbohydrates, lipids, and nucleic acids . Master Cell Biology with questions on organelle functions, such as the Mitochondria for energy production, the Smooth ER for detoxification, and Lysosomes for digestion . Detailed sections on Cellular Energetics explain the mechanics of Glycolysis, the Krebs cycle, and the Electron Transport Chain, as well as the light-dependent and Calvin cycle reactions in Photosynthesis . The guide also provides in-depth practice for Genetics and Heredity, including DNA replication, transcription, and translation . You will find exercises on Mendelian genetics, Punnett squares, and the distinct phases of Mitosis and Meiosis . Additionally, it covers the hierarchic system of classification and environmental biology concepts like symbiosis and the water/carbon cycles

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Hesi A2 Biology Exam Questions with Complete
Solutions.



Why is polarity the most important characteristic of water? - ANSWER-
The results of the polarity are hydrogen bonding, a high specific heat
value, and its versatile solvent properties.

Athletes are often concerned with the question of what they need in their
diets to increase muscle mass and strength. What biologic molecule
would you recommend that would accomplish this?
A. Carbohydrates
B. Proteins
C. Lipids
D. Nucleic acids - ANSWER-B. Proteins

Which organelle would you expect to be present in a cell responsible for
detoxifying multiple molecules?
A. Rough ER
B. Smooth ER
C. Lysosome
D. Golgi apparatus - ANSWER-B. Smooth ER

A cell from heart muscle would more than likely contain an usually high
proportion of:
A. Lysosomes
B. Mitochondria
C. mRNA
D. Ribosomes - ANSWER-B. Mitochondria

Which part of cell respiration produces the greatest amount of ATP?
A. ETC

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B. Glycolysis
C. Krebs cycle
D. Fermentation - ANSWER-A. ETC

When plants do not receive enough water their photosynthetic rated
drops. This is because:
A. Water is a raw material for the light reactions in photosynthesis.
B. Carbon dioxide is not available.
C. Water provides the carbon atoms used to make sugar.
D. Not enough oxygen is produced to keep fermentation running -
ANSWER-A. Water is a raw material for the light reactions in
photosynthesis

Which of the following statements is true about the Krebs cycle and the
Calvin cycle?
A. Both result in a net production of ATP and NADH
B. Both require a net input of ATP
C. Both result in release of oxygen
D. Both use light to initiate the process. - ANSWER-A. Both result in a
net production of ATP and NADH

Why is it important for cells to undergo mitosis? - ANSWER-Mitosis
produces cells for growth and repair of body tissue.

72 chromosomes undergo meiosis. How many chromosomes will be in
each gamete?
A. 18
B. 36
C. 72
D. 144 - ANSWER-B. 36

Which of the following shows how information is transformed to make a
protein?
A. DNA-RNA-protein
B. Gene-chromosome-protein

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C. ATP-amino acid-protein
D. RNA-DNA-protein - ANSWER-A. DNA-RNA-protein

In the hierarchic system of classification, which of the following is least
inclusive?
A. Kingdom
B. Class
C. Genus
D. Species - ANSWER-D. Species

After observing an event, you develop an explanation. This statement is
referred to as which of the following?
A. Hypothesis
B. Experiment
C. Conclusion
D. Theory - ANSWER-A. Hypothesis

A molecule of water is bonded with another molecule of water by what
type of bond?
A. Ionic
B. Covalent
C. Hydrogen
D. Molecular - ANSWER-C. Hydrogen

Which of the following is a benefit of the intermolecular hydrogen
bonding of water? (Select all that apply)
A. Water has a relatively high specific heat value.
B. Water has strong cohesive and adhesive properties.
C. Polarity of water allows it to act as a versatile solvent.
D. Water moves from higher to lower concentrations - ANSWER-A.
Water has a high specific heat
B. Water has strong cohesive and adhesive properties
C. Polarity of water allows it to act as a versatile solvent

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Of all the molecules that are significant to biology, which of the
following are considered the most important?
A. Carbohydrates, lipids, protein, and nucleic acids
B. Carbohydrates, lipids, protein, and calcium
C. Carbohydrates, lipids, protein, and sulfur
D. Carbohydrates, lipids, protein, and iron - ANSWER-A.
Carbohydrates, lipids, protein, and nucleic acids

Lipids are better known as fats, but what are they specifically? (Select
all that apply.)
A. Fatty acids
B. Phospholipids
C. Ketones
D. Steroids - ANSWER-A. Fatty acids
B. Phospholipids
D. Steroids

What are the two categories of fatty acids?
A. Trans fat and saturated fat
B. Trans fat and unsaturated fats
C. Saturated fats and unsaturated fats
D. Saturated fats and polyunsaturated fats - ANSWER-C. Saturated fats
and unsaturated fats

How do phospholipids function in cells?
A. They are integral components of the nuclear membrane.
B. They are integral components of the cytoplasmic skeleton.
C. They are integral components of the mitochondrial membranes.
D. They are integral components of the plasma membrane. - ANSWER-
D. They are integral components of the plasma membrane.

Which of the biologic molecules are considered the most significant
contributor to cellular function?
A. Carbohydrates
B. Lipids

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