AM
EXAM 2 REVIEW with well answered questions
2026/2027
1. What is one of the steps to ensure consistency when using the replica
plating technique?
Count the number of colonies before stamping
Make sure apparatus is oriented the same for each plate (north
plate) Perform the control stamp before any test stamping
Use different types of media for each plate
2. What method does the Bradford Assay utilize to measure protein concentration?
Chromatography
Fluorometry
Spectrophotometry
Electrophoresis
3. What does the term 'R group' refer to in the context of amino
acids? Peptide bond structure
Backbone of the amino acid
Variable portion of amino
acid Functional group of a
protein
4. In the context of the standard curve used in microbiology, what does the 'x'
axis typically represent?
Absorbance
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