SYBSC Minor practical ( External )
Study of Conducting Tissue (Maceration Technique)
Aim:- To study different conducting tissue in Gymnosperms & Angiosperms
through maceration technique.
Procedure :- A small piece of the given wood is taken in a hard glass test tube.
Concentrated HNO₃ is added to completely submerge the wood piece and
gently heated until the elements separate. The acid is removed by washing
thoroughly with water. The separated wood elements are stained with safranin
and excess stain is washed off. The elements are mounted in glycerin and
observed under the microscope.
Observations
Wood Elements of Annona ( Angiosperm )
The macerated wood of Annona shows few short vessels and many long fibres.
The vessels are broad and possess simple perforation plates. The lateral walls
show scalariform thickening. Fibres are very long, narrow, and have tapering
ends. The fibres are libriform type with simple pits. Since vessels are present,
the wood is angiospermic in nature.
Wood Elements of Sterculia ( Angiosperm )
The macerated wood of Sterculia shows well-developed vessels and numerous
fibres. The vessels are broad and long with simple perforation plates. The
lateral walls show pitted thickening. Fibres are long, narrow, and libriform type
with tapering ends. Simple pits are present on the fibres. As vessels are
present, the wood is angiospermic in nature.
Wood Elements of Thuja ( Gymnosperm )
The macerated wood of Thuja shows numerous tracheids and fibres. Vessels
are completely absent. Tracheids are long and narrow with pointed ends. The
lateral walls show uniseriate bordered pits. Perforation plates are absent. Since
vessels are absent, the wood is gymnospermic in nature.
Study of Conducting Tissue (Maceration Technique)
Aim:- To study different conducting tissue in Gymnosperms & Angiosperms
through maceration technique.
Procedure :- A small piece of the given wood is taken in a hard glass test tube.
Concentrated HNO₃ is added to completely submerge the wood piece and
gently heated until the elements separate. The acid is removed by washing
thoroughly with water. The separated wood elements are stained with safranin
and excess stain is washed off. The elements are mounted in glycerin and
observed under the microscope.
Observations
Wood Elements of Annona ( Angiosperm )
The macerated wood of Annona shows few short vessels and many long fibres.
The vessels are broad and possess simple perforation plates. The lateral walls
show scalariform thickening. Fibres are very long, narrow, and have tapering
ends. The fibres are libriform type with simple pits. Since vessels are present,
the wood is angiospermic in nature.
Wood Elements of Sterculia ( Angiosperm )
The macerated wood of Sterculia shows well-developed vessels and numerous
fibres. The vessels are broad and long with simple perforation plates. The
lateral walls show pitted thickening. Fibres are long, narrow, and libriform type
with tapering ends. Simple pits are present on the fibres. As vessels are
present, the wood is angiospermic in nature.
Wood Elements of Thuja ( Gymnosperm )
The macerated wood of Thuja shows numerous tracheids and fibres. Vessels
are completely absent. Tracheids are long and narrow with pointed ends. The
lateral walls show uniseriate bordered pits. Perforation plates are absent. Since
vessels are absent, the wood is gymnospermic in nature.
