ASCP CG CERTIFICATION EXAM 2025 WITH 200 REAL EXAM PRACTICE QUESTIONS AND CORRECT ANSWERS/ ASCP CG CERTIFICATION TEST 2025/ ASCP CYTOGENETICS TEST

ASCP CG CERTIFICATION EXAM 2025 WITH 200 REAL EXAM
PRACTICE QUESTIONS AND CORRECT ANSWERS/ ASCP CG
CERTIFICATION TEST 2025/ ASCP CYTOGENETICS TEST

What is the best agent to use against mycoplasma? - ANSWER-BM cyclin - a combination of two
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antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
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For successful harvest, each new batch of hypotonic solution should be evaluated by: -
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ANSWER- comparing the new lot to a lot already in use
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Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes - break down tissues
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into single-cell suspensions
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When harvesting peripheral blood cultures, asceptic technique no longer matters following
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incubation with: - ANSWER-Colcemid - mitotic spindle poison that halts cells at metaphase
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When scoring FISH for DiGeorge syndrome, a tech notices the TUPLE1 signal is present, but one
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of the control ARSA probe signals is missing in every cell. This result suggests ? - ANSWER-
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The patient has a deletion of the ARSA region
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CLIA 1988- (Clinical Laboratory Improvement Amendments) stipulates that equipment must
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be recalibrated
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What type of stained chromosome would produce a better computer image? - ANSWER-Lightly
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stained chromosomes (best contrast and can be digitally edited easier)
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What is the nomenclature on a patient with down syndrome referred for a hematologic disorder
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where only trisomy 21 was found? - ANSWER-47,XY,+21c[20]
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24 hours after culture initiation, a single dish from an amniotic fluid turns cloudy. Which cultures
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should be disposed? - ANSWER-The single cloudy dish only
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,What is used to reduce mycoplasma contamination? - ANSWER-BM cycline
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CLIA '88 stipulates that equipment must be recalibrated
M M M M M M M M ? - ANSWER-After a major repair to
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ensure still operational
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How should caustic and corrosive chemicals be stored? - ANSWER-Both should be stored in a
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nonflammable cabinet
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What factors would contribute to ALL cultures failing in a CO2 incubator? - ANSWER-Temperature
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CO2 level
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What is the modal number for a diploid karyotype and an additional CBG negative marker
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chromosome? - ANSWER-46
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CBG = c-bands achieved with barium with Giemsa stain
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CBG negative referrs to not having a centromere, which is crucial to being included in the modal
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number, so this extra fragment does not have a centromere and is not counted towards the total
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What is included when recording the modal number? - ANSWER-Mar - a marker chromosome is
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included because it has a centromere
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What is the BEST way to avoid producing ghost-like, pale chromosomes while staining? -
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ANSWER- Decrease trypsin time
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In order to ensure successful harvest, each new batch of hypotonic solution should be evaluated
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. - ANSWER-By comparing new hypotonic solution to that already in use
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Which cell type would you want to use for long term serial cytogenetic studies? - ANSWER-
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Fibroblast cells
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, What is not a necessary (must be added) ingredient which is added to modified medium? -
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ANSWER-Antibiotics


What chemical is used for cryopreservation? - ANSWER-Dimethyl sulfoxide (DMSO)
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You are analyzing a clinical case and discover a small extra marker with satellites. What staining
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technique would be most useful in determining the chromosomal origin of this marker? -
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ANSWER-NOR


When using a fluorescein dye (yellow-green) labeled probe during the FISH procedure, which
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counterstain should be used? - ANSWER-Propidium iodide (red)
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A bone marrow sample collected in transport medium is leaking into the sealed plastic bag in
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which it was transported. The technologist should . -
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wearing gloves
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A laboratory' quality assurance program includes
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All of the following chromosomes have dark bands on chromosome regions that can be used as
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indicators when preparing the best image: - ANSWER-Chromosome 11p
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Chromosome 12q
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The morning after being set-up, a single dish of an amniotic fluid culture turns cloudy and
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appears contaminated. None of the other cultures set-up that day appears contaminated.
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What should be done first? - ANSWER-Harvest the culture as soon as possible
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When the addition of nonhomologous material to the q-arm of chromosome 16 is suspected after
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G- banding, what banding technique should be used? - ANSWER-C-banding
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What chromosome is most different between C- and G-banding? - ANSWER-Chromosome 9
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What chromosome region does quinacrine and acridine orange dyes stain? - ANSWER-AT rich DNA
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