2025 – 2026 Questions From Actual Exams
Complete 100 Questions And Correct Detailed
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Content Covered (Most Tested Areas)
Forensic evidence handling and chain of custody
Crime scene processing and documentation
Forensic biology and DNA analysis
Forensic toxicology
Controlled substances analysis
Trace evidence examination
Forensic microscopy and instrumentation
Quality assurance and laboratory accreditation
Ethics and professional standards
Courtroom testimony and expert witness procedures
1. Which principle states that every contact leaves a trace in forensic
investigations?
A. Frye Standard
B. Locard’s Exchange Principle
C. Kirk’s Law
D. Newton’s Law
Correct Answer: Locard’s Exchange Principle
,Explanation: Locard’s Exchange Principle states that whenever two objects come
into contact, there is an exchange of materials. This concept forms the basis of
forensic evidence transfer at crime scenes.
2. The primary purpose of maintaining a chain of custody is to:
A. Identify suspects
B. Determine cause of death
C. Document the handling of evidence from collection to court
D. Establish the time of crime
Correct Answer: Document the handling of evidence from collection to court
Explanation: Chain of custody ensures that evidence integrity is preserved by
documenting every individual who handled the evidence from collection
through analysis and presentation in court.
3. Which instrument is most commonly used to separate chemical mixtures in
forensic toxicology?
A. Microscope
B. Spectrophotometer
C. Gas Chromatograph
D. Centrifuge
Correct Answer: Gas Chromatograph
Explanation: Gas chromatography separates volatile compounds within mixtures
and is widely used in toxicology for identifying drugs, alcohol, and poisons.
4. What type of biological sample is most commonly used for DNA profiling?
A. Saliva only
B. Bone fragments
C. Blood
D. Hair without root
Correct Answer: Blood
,Explanation: Blood is commonly used because it contains nucleated cells that
provide sufficient DNA for profiling.
5. The standard used in many courts to determine the admissibility of
scientific evidence based on reliability is the:
A. Miranda Rule
B. Best Evidence Rule
C. Daubert Standard
D. Brady Rule
Correct Answer: Daubert Standard
Explanation: The Daubert Standard requires judges to evaluate whether
scientific evidence is reliable and relevant before allowing expert testimony.
6. Which type of evidence consists of small materials such as fibers, glass, or
paint?
A. Biological evidence
B. Chemical evidence
C. Trace evidence
D. Documentary evidence
Correct Answer: Trace evidence
Explanation: Trace evidence includes minute materials that transfer during
physical contact, such as fibers, glass fragments, or soil.
7. In forensic drug analysis, a presumptive test is primarily used to:
A. Confirm chemical structure
B. Determine purity
C. Indicate the possible presence of a drug
D. Determine dosage levels
Correct Answer: Indicate the possible presence of a drug
Explanation: Presumptive tests provide quick screening results that suggest the
presence of certain substances but require confirmatory testing.
, 8. The most commonly used confirmatory technique for controlled substances
analysis is:
A. Thin layer chromatography
B. Gas chromatography–mass spectrometry
C. UV light examination
D. Refractometry
Correct Answer: Gas chromatography–mass spectrometry
Explanation: GC-MS provides highly reliable compound identification by
combining separation and molecular analysis.
9. In forensic biology, the process of copying DNA segments millions of times
is called:
A. Electrophoresis
B. Hybridization
C. Polymerase Chain Reaction
D. Chromatography
Correct Answer: Polymerase Chain Reaction
Explanation: PCR amplifies specific DNA segments, enabling forensic scientists to
analyze small biological samples.
10.The purpose of electrophoresis in DNA analysis is to:
A. Break DNA strands
B. Detect proteins
C. Separate DNA fragments by size
D. Measure pH
Correct Answer: Separate DNA fragments by size
Explanation: Electrophoresis separates DNA fragments through a gel matrix
under an electric field according to size.
11.Which document records observations and actions taken at a crime scene?