BCS 200 Lab Practical Exam questions and
answers Graded A+
Safety equipment required for students to wear in lab - ✅✅safety glass and lab
coat (disposable)
GHS (Global Harmonized System) Labels - ✅✅
Fire Extinguisher PASS - ✅✅pull the pin
aim at the base of the fire
squeeze the lever slowly
sweep from side to side
non-infectious/non-biohazard, non-sharps - ✅✅regular trash container
human saliva on cotton tip applicator
non-infectious/non-biohazard, SHARPS - ✅✅white and blue cardboard box
with clear liner
glass slides go here
infectious/biohazard, non-sharps - ✅✅red oval plastic container with bright
yellow liner
plastic test tubes containing a liquid bacterial culture
infectious/biohazard sharps - ✅✅red squared plastic container with no liner
, precautions - ✅✅person protective equipment (lab coat, safety glasses, and
gloves), closed-toe shoes, discard materials in appropriate containers, surfaces
are cleaned before and after use
streak plate method - ✅✅used to grow bacteria on a growth media surface so
that individual bacterial colonies are isolated and sampled
spread plate method - ✅✅used to evenly distribute bacteria across the plate to
make the calculation of the number of bacteria colonies easier
stab and streak method - ✅✅done to maximize the surface area available,
while being able to observe two or more aspects of the test (color change, gas
production, carbohydrate utilization, etc)
bacterial lawn method - ✅✅transferring the environmental sample to the agar
plate
aseptic technique - ✅✅used for a variety of procedures such as transferring
cultures, inoculating media, isolation of pure cultures, and for performing
microbiological tests
proper aseptic technique prevents contamination of cultures from foreign
bacteria inherent in the environment
proper aseptic technique can greatly minimize or even eliminate the risk of
contamination
Incubation temperatures - ✅✅25 degrees Celsius (room temp)
answers Graded A+
Safety equipment required for students to wear in lab - ✅✅safety glass and lab
coat (disposable)
GHS (Global Harmonized System) Labels - ✅✅
Fire Extinguisher PASS - ✅✅pull the pin
aim at the base of the fire
squeeze the lever slowly
sweep from side to side
non-infectious/non-biohazard, non-sharps - ✅✅regular trash container
human saliva on cotton tip applicator
non-infectious/non-biohazard, SHARPS - ✅✅white and blue cardboard box
with clear liner
glass slides go here
infectious/biohazard, non-sharps - ✅✅red oval plastic container with bright
yellow liner
plastic test tubes containing a liquid bacterial culture
infectious/biohazard sharps - ✅✅red squared plastic container with no liner
, precautions - ✅✅person protective equipment (lab coat, safety glasses, and
gloves), closed-toe shoes, discard materials in appropriate containers, surfaces
are cleaned before and after use
streak plate method - ✅✅used to grow bacteria on a growth media surface so
that individual bacterial colonies are isolated and sampled
spread plate method - ✅✅used to evenly distribute bacteria across the plate to
make the calculation of the number of bacteria colonies easier
stab and streak method - ✅✅done to maximize the surface area available,
while being able to observe two or more aspects of the test (color change, gas
production, carbohydrate utilization, etc)
bacterial lawn method - ✅✅transferring the environmental sample to the agar
plate
aseptic technique - ✅✅used for a variety of procedures such as transferring
cultures, inoculating media, isolation of pure cultures, and for performing
microbiological tests
proper aseptic technique prevents contamination of cultures from foreign
bacteria inherent in the environment
proper aseptic technique can greatly minimize or even eliminate the risk of
contamination
Incubation temperatures - ✅✅25 degrees Celsius (room temp)
