Test Bank for AP Biology –
Chapter 20 (All Questions
and Answers Included)
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Page 1 of 14 Test Bank for AP Biology – Chapter 20 (All Questions and Answers Included).pdf
, AP Biology - Chapter 20 Test Bank Page 2 2026-03-12
Bacterial cells protect their own DNA from restriction enzymes (endonucleases) by _____.
adding methyl groups to adenines and cytosines
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a
bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.
III, II, IV, V, I
A principal problem with inserting an unmodified mammalian gene into a plasmid and then getting that gene
expressed in bacteria is that _____.
bacteria cannot remove eukaryotic introns
Yeast cells are frequently used as hosts for cloning because they _____.
are eukaryotic cells
Page 2 of 14 2 AP Biology - Chapter 20 Test Bank.pdf