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TABLE OF CONTENTS
1. Introduction — Page 1
2. Core Domain 1: Phlebotomy Fundamentals — Page 2
3. Core Domain 2: Anatomy and Physiology for Phlebotomy — Page 3
4. Core Domain 3: Specimen Collection Procedures — Page 4
5. Core Domain 4: Safety, Infection Control, and OSHA Compliance — Page 5
6. Core Domain 5: Specimen Handling and Laboratory Processing — Page 6
7. Core Domain 6: Patient Identification and Professional Communication — Page 7
8. Core Domain 7: Legal, Ethical, and Regulatory Standards — Page 8
9. Core Domain 8: Complications and Special Collections — Page 9
10. Answer Key Summary — Page 10
CORE DOMAINS
1. Phlebotomy Fundamentals
, 2. Anatomy and Physiology for Phlebotomy
3. Specimen Collection Procedures
4. Safety, Infection Control, and OSHA Compliance
5. Specimen Handling and Laboratory Processing
6. Patient Identification and Professional Communication
7. Legal, Ethical, and Regulatory Standards
8. Complications and Special Collections
INTRODUCTION
The Certified Phlebotomy Technician (CPT) examination evaluates the knowledge and
technical skills required to safely and effectively collect blood specimens for diagnostic
testing. Candidates are assessed on foundational phlebotomy theory, venipuncture
procedures, infection control standards, specimen handling protocols, and professional
conduct within clinical environments. The exam uses multiple-choice and scenario-based
questions to measure the ability to apply practical knowledge in real-world healthcare
settings. Emphasis is placed on patient safety, regulatory compliance, accurate specimen
collection, and ethical decision-making. Successful candidates demonstrate competence in
laboratory procedures, communication with patients, and adherence to professional
standards expected of phlebotomy technicians.
,SECTION 1: QUESTIONS 1–35
1. What is the primary purpose of performing venipuncture?
A. To administer medication
B. To remove toxins from the bloodstream
🔴 C. To collect blood specimens for laboratory testing
D. To measure blood pressure
🔵 Explanation: Venipuncture is performed primarily to obtain blood samples for
diagnostic laboratory testing.
2. Which vein is most commonly used for routine venipuncture?
A. Cephalic vein
🔴 B. Median cubital vein
C. Basilic vein
D. Radial vein
🔵 Explanation: The median cubital vein is preferred because it is usually large, well
anchored, and less prone to rolling.
3. Which organization sets standards for workplace safety regarding bloodborne
pathogens?
A. CDC
🔴 B. OSHA
, C. FDA
D. WHO
🔵 Explanation: The Occupational Safety and Health Administration (OSHA) regulates
workplace safety and bloodborne pathogen standards.
4. What is the correct order of draw during venipuncture?
A. EDTA, citrate, serum
B. Serum, heparin, citrate
🔴 C. Blood cultures, citrate, serum, heparin, EDTA
D. EDTA, serum, citrate
🔵 Explanation: The standard order of draw prevents additive carryover that could alter
laboratory results.
5. What is the first step before performing venipuncture?
🔴 A. Verify patient identity
B. Prepare tubes
C. Apply tourniquet
D. Clean the puncture site
🔵 Explanation: Patient identification is critical to ensure specimen accuracy and patient
safety.