1 | Page
ASCP CG CERTIFICATION 2026 100% PASS
ASSURED EXAMINATION SOLUTIONS
What is the best agent to use against
mycoplasma? - correct answer- BM cyclin - a
combination of two antibiotics (tiamulin and
minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of
hypotonic solution should be evaluated by: -
correct answer- comparing the new lot to a lot
already in use
Colagenase, pronase, and trypsin are: -
correct answer- Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures,
asceptic technique no longer matters following
incubation with: - correct answer- Colcemid -
mitotic spindle poison that halts cells at
metaphase
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Samples that arrive at the cytogenetics lab
should first be: - correct answer- Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type
of cells into division? - correct answer- T-
cell lymphocytes
To avoid culture failure, monitoring reagents
is crucial. When logging a culture failure,
what is the MOST important to document? -
correct answer- Expiration dates of reagents
used
Which type of microscope is used when cleaning
a CVS (chorionic villus) sample? - correct
answer- Dissection
Which cell type is most problematic if found
in a CVS (chorionic villus) sample? - correct
answer- Decidua (maternal tissue)
,3 | Page
When looking at a slide under a phase contrast
scope and a lot of cytoplasm is still seen
around the mets, what is the most likely
cause? - correct answer- innappropriate slide
drying time
What will help prevent microbial contamination
and cross contamination of cultures? - correct
answer- Asceptic technique
When there are not enough mets to complete the
study of a patient/case, what needs to be done
next? - correct answer- prepare additional
slides from any remaining sample
When looking at a dropped slide under a phase
scope and mets are very spread out and sparse,
what could help fix the issue? - correct
answer- Increading the drying time by using
warm slides
What is true of an inverted microscope? -
correct answer- the objectives are beneath the
stage
, 4 | Page
High-resolution banding is indicated for a
possible diagnosis of: - correct answer-
DiGenorge syndrome -- microdeletion of 22q11,
also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features,
short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate
for cytogenetic analysis, what is the MOST
important data to accompany the specimen? -
correct answer- Reason for referral
Which type of specimen should be requested for
a cytogenetic analysis for turner syndrome? -
correct answer- Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
ASCP CG CERTIFICATION 2026 100% PASS
ASSURED EXAMINATION SOLUTIONS
What is the best agent to use against
mycoplasma? - correct answer- BM cyclin - a
combination of two antibiotics (tiamulin and
minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of
hypotonic solution should be evaluated by: -
correct answer- comparing the new lot to a lot
already in use
Colagenase, pronase, and trypsin are: -
correct answer- Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures,
asceptic technique no longer matters following
incubation with: - correct answer- Colcemid -
mitotic spindle poison that halts cells at
metaphase
,2 | Page
Samples that arrive at the cytogenetics lab
should first be: - correct answer- Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type
of cells into division? - correct answer- T-
cell lymphocytes
To avoid culture failure, monitoring reagents
is crucial. When logging a culture failure,
what is the MOST important to document? -
correct answer- Expiration dates of reagents
used
Which type of microscope is used when cleaning
a CVS (chorionic villus) sample? - correct
answer- Dissection
Which cell type is most problematic if found
in a CVS (chorionic villus) sample? - correct
answer- Decidua (maternal tissue)
,3 | Page
When looking at a slide under a phase contrast
scope and a lot of cytoplasm is still seen
around the mets, what is the most likely
cause? - correct answer- innappropriate slide
drying time
What will help prevent microbial contamination
and cross contamination of cultures? - correct
answer- Asceptic technique
When there are not enough mets to complete the
study of a patient/case, what needs to be done
next? - correct answer- prepare additional
slides from any remaining sample
When looking at a dropped slide under a phase
scope and mets are very spread out and sparse,
what could help fix the issue? - correct
answer- Increading the drying time by using
warm slides
What is true of an inverted microscope? -
correct answer- the objectives are beneath the
stage
, 4 | Page
High-resolution banding is indicated for a
possible diagnosis of: - correct answer-
DiGenorge syndrome -- microdeletion of 22q11,
also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features,
short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate
for cytogenetic analysis, what is the MOST
important data to accompany the specimen? -
correct answer- Reason for referral
Which type of specimen should be requested for
a cytogenetic analysis for turner syndrome? -
correct answer- Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
