ASCP(MB) (BY SMOCKUS), MB (ASCP) PRACTICE
EXAM QUESTIONS AND ANSWERS
Genes involved in cancer caused by t(9;22) - Answers -BCR-ABL
Cancer caused by t(9:22) - Answers -CML
Genes involved in cancer caused by t(11;22) - Answers -EWS-FLI1
Cancer caused by translocations involving 22q12 - Answers -Ewing sarcoma
Receptor tyrosine kinase commonly overexpressed in breast cancer - Answers -
HER2(ERBB2) - 17q21.1
Methods used to assess overexpression of common oncogenes - Answers -IHC,
rtPCR, FISH
Most common oncogene in human cancer, which activates MAPK pathway - Answers -
KRAS
Most common translocation in 85% of Ewing's sarcoma - Answers -t(11,22)(q24,q12)
80% of this type of cancer is caused by t(X;18) - Answers -Synovial sarcoma
Inherited mutations in this gene cause Li-Fraumeni syndrome - Answers -TP53
Mutations in this gene increase lifetime risk of developing breast or ovarian cancer to
60-80% - Answers -BRCA1
Point mutations in this gene cause multiple endocrine neoplasia - Answers -RET
Translocations involving this gene commonly cause papillary carcinoma - Answers -
RET
Alterations in this gene lead to a syndrome, which predisposes to renal cell carcinoma -
Answers -VHL
This type of cancer is associated with a defective mismatch repair system (MMR) -
Answers -Colon cancer
MSI is often associated (90%) with this type of inherited cancer - Answers -Hereditary
nonpolyposis colorectal cancer (HNPCC) aka Lynch syndrome
How many repeat loci are analyzed to determine MSI? - Answers -Five
, In MSI testing 2 of 5 loci must show instability to classify it to this degree (MSH-H or
MSH-L) - Answers -High
Two gene associated with the cancer, HNPCC - Answers -MSH2 and MLH1
A ratio of less than ____ and greater than ____ indicates of loss of heterozygosity
(LOH) - Answers -0.5 and 2
Common translocation in follicular lymphoma - Answers -t(14;18)
This gene is often involved in translocations in follicular lymphoma - Answers -BCL2
This translocation is also known as the Philadelphia chromosome - Answers -t(9;22)
Two genes commonly translocated in acute promyelocytic leukemia - Answers -
PML/RARA
Common translocation in acute promyelocytic leukemia - Answers -t(15;17)
Lymphocyte clonality is detected by this method - Answers -Southern blot
When over 1% of cells make the same gene rearrangement, the cell population is called
________. - Answers -Monoclonal
The major histocompatability complex (MHC) is found on this chromosome - Answers -
6
MHC gene products in humans are called __________. - Answers -HLA; human
leukocyte antigens
HLA or tissue typing prevents _____________. - Answers -GVHD; graft versus host
disease
Haplotype - Answers -Set of alleles on the same chromosome and inherited together
Polymorphism - Answers -Alterations shared in DNA by at least 2% of the population
Allele - Answers -Different versions of a gene
Initial identification of HLA alleles in a specimen - Answers -HLA Typing
Detection of antihuman antibodies in serum that match known HLA alleles - Answers -
HLA Screening
EXAM QUESTIONS AND ANSWERS
Genes involved in cancer caused by t(9;22) - Answers -BCR-ABL
Cancer caused by t(9:22) - Answers -CML
Genes involved in cancer caused by t(11;22) - Answers -EWS-FLI1
Cancer caused by translocations involving 22q12 - Answers -Ewing sarcoma
Receptor tyrosine kinase commonly overexpressed in breast cancer - Answers -
HER2(ERBB2) - 17q21.1
Methods used to assess overexpression of common oncogenes - Answers -IHC,
rtPCR, FISH
Most common oncogene in human cancer, which activates MAPK pathway - Answers -
KRAS
Most common translocation in 85% of Ewing's sarcoma - Answers -t(11,22)(q24,q12)
80% of this type of cancer is caused by t(X;18) - Answers -Synovial sarcoma
Inherited mutations in this gene cause Li-Fraumeni syndrome - Answers -TP53
Mutations in this gene increase lifetime risk of developing breast or ovarian cancer to
60-80% - Answers -BRCA1
Point mutations in this gene cause multiple endocrine neoplasia - Answers -RET
Translocations involving this gene commonly cause papillary carcinoma - Answers -
RET
Alterations in this gene lead to a syndrome, which predisposes to renal cell carcinoma -
Answers -VHL
This type of cancer is associated with a defective mismatch repair system (MMR) -
Answers -Colon cancer
MSI is often associated (90%) with this type of inherited cancer - Answers -Hereditary
nonpolyposis colorectal cancer (HNPCC) aka Lynch syndrome
How many repeat loci are analyzed to determine MSI? - Answers -Five
, In MSI testing 2 of 5 loci must show instability to classify it to this degree (MSH-H or
MSH-L) - Answers -High
Two gene associated with the cancer, HNPCC - Answers -MSH2 and MLH1
A ratio of less than ____ and greater than ____ indicates of loss of heterozygosity
(LOH) - Answers -0.5 and 2
Common translocation in follicular lymphoma - Answers -t(14;18)
This gene is often involved in translocations in follicular lymphoma - Answers -BCL2
This translocation is also known as the Philadelphia chromosome - Answers -t(9;22)
Two genes commonly translocated in acute promyelocytic leukemia - Answers -
PML/RARA
Common translocation in acute promyelocytic leukemia - Answers -t(15;17)
Lymphocyte clonality is detected by this method - Answers -Southern blot
When over 1% of cells make the same gene rearrangement, the cell population is called
________. - Answers -Monoclonal
The major histocompatability complex (MHC) is found on this chromosome - Answers -
6
MHC gene products in humans are called __________. - Answers -HLA; human
leukocyte antigens
HLA or tissue typing prevents _____________. - Answers -GVHD; graft versus host
disease
Haplotype - Answers -Set of alleles on the same chromosome and inherited together
Polymorphism - Answers -Alterations shared in DNA by at least 2% of the population
Allele - Answers -Different versions of a gene
Initial identification of HLA alleles in a specimen - Answers -HLA Typing
Detection of antihuman antibodies in serum that match known HLA alleles - Answers -
HLA Screening
