MB ASCP EXAM 1 QUESTIONS AND ANSWERS
What is the function of DNA? - Answers -Store genetic information
Purine - Answers -Double ring of nitrogen base (G, A)
Pyrimidine - Answers -Single ring of nitrogen base (T, C)
What is the complementary sequence to 5'AGGTCA3' ? - Answers -5'TGACCT3'
Which of the ribose carbons participate in the phosphodiester bond? - Answers -The 5'
ribose carbon carries the phosphate group that forms a phosphodiester bond with the
hydroxyl group on the 3' ribose carbon.
Which ribose carbon carries the nitrogen base? - Answers -The 1' carbon
Why does DNA polymerase require primase activity? - Answers -DNA polymerase
cannot begin synthesis without a 3' OH group (thus DNA is read 3' to 5' to synthesize
new DNA)
Phosphodiester bond is... - Answers -the covalent bond between nucleotides catalyzed
by DNA polymerase
DNA replication is conservative or semi-conservative? - Answers -Semi-conservative
What is the lagging strand in DNA synthesis? - Answers -The strand positioned 5' to 3'
with respect to the direction of synthesis, requiring the replication assembly to jump
ahead and read 3' to 5' back toward the moving replication fork. The leading strand is
read continuously 3' to 5'.
In what direction is DNA synthesized? - Answers -The newly synthesized DNA is
created 5' to 3. The template DNA is read 3' to 5'.
Okazaki Fragments - Answers -Short pieces of DNA involved in DNA synthesis
Polymerase - Answers -Catalyzes the formation of a phosphodiester bond between
nucleotides
Helicase - Answers -Separates the two strands of DNA
Primase - Answers -An RNA polymerase that starts replication in the absence of a 3'
OH group
What is the function of DNA? - Answers -Store genetic information
Purine - Answers -Double ring of nitrogen base (G, A)
Pyrimidine - Answers -Single ring of nitrogen base (T, C)
What is the complementary sequence to 5'AGGTCA3' ? - Answers -5'TGACCT3'
Which of the ribose carbons participate in the phosphodiester bond? - Answers -The 5'
ribose carbon carries the phosphate group that forms a phosphodiester bond with the
hydroxyl group on the 3' ribose carbon.
Which ribose carbon carries the nitrogen base? - Answers -The 1' carbon
Why does DNA polymerase require primase activity? - Answers -DNA polymerase
cannot begin synthesis without a 3' OH group (thus DNA is read 3' to 5' to synthesize
new DNA)
Phosphodiester bond is... - Answers -the covalent bond between nucleotides catalyzed
by DNA polymerase
DNA replication is conservative or semi-conservative? - Answers -Semi-conservative
What is the lagging strand in DNA synthesis? - Answers -The strand positioned 5' to 3'
with respect to the direction of synthesis, requiring the replication assembly to jump
ahead and read 3' to 5' back toward the moving replication fork. The leading strand is
read continuously 3' to 5'.
In what direction is DNA synthesized? - Answers -The newly synthesized DNA is
created 5' to 3. The template DNA is read 3' to 5'.
Okazaki Fragments - Answers -Short pieces of DNA involved in DNA synthesis
Polymerase - Answers -Catalyzes the formation of a phosphodiester bond between
nucleotides
Helicase - Answers -Separates the two strands of DNA
Primase - Answers -An RNA polymerase that starts replication in the absence of a 3'
OH group
