BIOL 2270 Final Lab Report – Kirby-Bauer Antimicrobial Test (Minnesota
State University)
BIOL 2270 Lab – Kirby-Bauer
Antimicrobial Susceptibility
(Virtual Lab)
This virtual lab will introduce you to the Kirby-Bauer antimicrobial susceptibility test.
Using Michigan State University’s Virtual Interactive Bacteriology Laboratory, you will explore how this
test is done and what the results mean.
Click on “Kirby-Bauer” then on the module link (the module will open in a new tab).
Introduction
1. Which organization has standardized the Kirby-Bauer test in the US?
-World Health Organization
2. What three results are possible when the zones of inhibition are interpreted using the
standardized table?
a. resistive
b. intermediate
c. susceptible
Click on the module link (the module will open in a new tab) and read the description.
3. What is the name of the agar plate used in the Kirby-Bauer test?
- Mueller Hinton agar was used.
4. What is on the disks?
- Bacterias and antibiotics
Go back to the “menu” and read the “steps.” Once you feel comfortable with the step, go back to the
“menu,” and click on “start.” Compete the Kirby-Bauer test.
There are three different bacteria species. Make sure to do all three by clicking on “menu” and then
“restart.”
1. What is the bacteria species and date?
, - E.coli date: 11/25/07
2. Insert a screenshot of your results OR Place a R (resistant), I (intermediate) or S (susceptible)
after each of the antibiotics:
Amoxicillin (AMC) - R
Cephalothin (CF) - S
Chloramphenicol (C) - S
Ciprofloxacin (CIP) - S
Clindamycin (CC) - R
Erythromycin (E) - R
Oxacillin (OX) - R
Penicillin G (P) - R
Streptomycin (S) -S
Tetracycline (TE) - I
Tobramycin (TM) - S
Trimethoprim sulfa (SXT) –S
3. What is the bacteria species and date?
- PA: 11/25/07
4. Insert a screenshot of your results OR Place a R (resistant), I (intermediate) or S (susceptible)
after each of the antibiotics:
State University)
BIOL 2270 Lab – Kirby-Bauer
Antimicrobial Susceptibility
(Virtual Lab)
This virtual lab will introduce you to the Kirby-Bauer antimicrobial susceptibility test.
Using Michigan State University’s Virtual Interactive Bacteriology Laboratory, you will explore how this
test is done and what the results mean.
Click on “Kirby-Bauer” then on the module link (the module will open in a new tab).
Introduction
1. Which organization has standardized the Kirby-Bauer test in the US?
-World Health Organization
2. What three results are possible when the zones of inhibition are interpreted using the
standardized table?
a. resistive
b. intermediate
c. susceptible
Click on the module link (the module will open in a new tab) and read the description.
3. What is the name of the agar plate used in the Kirby-Bauer test?
- Mueller Hinton agar was used.
4. What is on the disks?
- Bacterias and antibiotics
Go back to the “menu” and read the “steps.” Once you feel comfortable with the step, go back to the
“menu,” and click on “start.” Compete the Kirby-Bauer test.
There are three different bacteria species. Make sure to do all three by clicking on “menu” and then
“restart.”
1. What is the bacteria species and date?
, - E.coli date: 11/25/07
2. Insert a screenshot of your results OR Place a R (resistant), I (intermediate) or S (susceptible)
after each of the antibiotics:
Amoxicillin (AMC) - R
Cephalothin (CF) - S
Chloramphenicol (C) - S
Ciprofloxacin (CIP) - S
Clindamycin (CC) - R
Erythromycin (E) - R
Oxacillin (OX) - R
Penicillin G (P) - R
Streptomycin (S) -S
Tetracycline (TE) - I
Tobramycin (TM) - S
Trimethoprim sulfa (SXT) –S
3. What is the bacteria species and date?
- PA: 11/25/07
4. Insert a screenshot of your results OR Place a R (resistant), I (intermediate) or S (susceptible)
after each of the antibiotics: