Bio Lab Exam II Updated Questions And
Answers With 100% Correct Answers
Graded A+
QUESTIONS AND ANSWERS
Why do the cheek cells need to be lysed before setting up PCR? ANSWER - Lysing breaks
open the cells so the DNA is accessible.
What does PCR stand for? ANSWER - Polymerase chain reaction
Which of the following will be components in your PCR mixture? ANSWER - Master mix
Human DNA
What are you trying to amplify using PCR in this experiment? ANSWER - Short tandem
repeats from specific genes
Match the definitions with the PCR terminology.
Denaturation
Annealing
Extension
Thermocycler ANSWER - Denaturation: The DNA template is heated so the strands
separate.
Annealing: The reaction is cooled so the primers can stick to the DNA template.
, Extension: The temperature is changed to the ideal temperature for Taq polymerase to
build (synthesize) a new strand based on the template.
Thermocycler:
The machine that controls the temperature of the reaction and has been programmed to
move through the heating and cooling steps.
When you arrive at lab, you need to be wearing [a] shoes. When it is time to start
experiments, you must wear [b] and, to keep you safe, they must remain over your [c]
while [d] in the lab is doing experiments. ANSWER - When you arrive at lab, you need to
be wearing close-toed shoes. When it is time to start experiments, you must wear goggles
and, to keep you safe, they must remain over your eyes while anyone in the lab is doing
experiments.
Match the function to the name of the lab equipment.
Centrifuge
Thermocycler
Micropipette
Chelex beads ANSWER - Centrifuge: Spins at a high speed to separate things by density
(denser stuff moves lower in the tube).
Thermocycler: Can be programmed to keep samples/reactions at specific temperatures for
specific lengths of time.
Micropipette: Measures and transfers very specific, usually small, volumes of fluid
accurately.
Chelex beads: Bind metal ions so they can be removed from the sample.
[x] uses a/an [y] called Taq polymerase to create [z] of copies of a specific region of [w].
ANSWER - [PCR] uses a/an [enzyme] called Taq polymerase to create [thousands] of
copies of a specific region of [DNA].
Answers With 100% Correct Answers
Graded A+
QUESTIONS AND ANSWERS
Why do the cheek cells need to be lysed before setting up PCR? ANSWER - Lysing breaks
open the cells so the DNA is accessible.
What does PCR stand for? ANSWER - Polymerase chain reaction
Which of the following will be components in your PCR mixture? ANSWER - Master mix
Human DNA
What are you trying to amplify using PCR in this experiment? ANSWER - Short tandem
repeats from specific genes
Match the definitions with the PCR terminology.
Denaturation
Annealing
Extension
Thermocycler ANSWER - Denaturation: The DNA template is heated so the strands
separate.
Annealing: The reaction is cooled so the primers can stick to the DNA template.
, Extension: The temperature is changed to the ideal temperature for Taq polymerase to
build (synthesize) a new strand based on the template.
Thermocycler:
The machine that controls the temperature of the reaction and has been programmed to
move through the heating and cooling steps.
When you arrive at lab, you need to be wearing [a] shoes. When it is time to start
experiments, you must wear [b] and, to keep you safe, they must remain over your [c]
while [d] in the lab is doing experiments. ANSWER - When you arrive at lab, you need to
be wearing close-toed shoes. When it is time to start experiments, you must wear goggles
and, to keep you safe, they must remain over your eyes while anyone in the lab is doing
experiments.
Match the function to the name of the lab equipment.
Centrifuge
Thermocycler
Micropipette
Chelex beads ANSWER - Centrifuge: Spins at a high speed to separate things by density
(denser stuff moves lower in the tube).
Thermocycler: Can be programmed to keep samples/reactions at specific temperatures for
specific lengths of time.
Micropipette: Measures and transfers very specific, usually small, volumes of fluid
accurately.
Chelex beads: Bind metal ions so they can be removed from the sample.
[x] uses a/an [y] called Taq polymerase to create [z] of copies of a specific region of [w].
ANSWER - [PCR] uses a/an [enzyme] called Taq polymerase to create [thousands] of
copies of a specific region of [DNA].
