ASCP CG LATEST
CERTIFICATION EXAM
Which of the following ARE advantages of FISH diagnostics concerning
interphase testing? - ANSWERS-Can rapidly detect sex chromosomes or
aneuploidy of chromosomes.
Requires no culture
Requires only simple microscope analysis.
The temperature of -20oC is necessary in which of the following FISH
procedures? - ANSWERS-Probe storage
The temperature of 4oC is necessary in which of the following FISH
procedures? - ANSWERS-FITC-avidin storage
The temperature of 90oC is necessary in which of the following FISH
procedures? - ANSWERS-simultaneous denaturation
Most important pretreatment to age slides for FISH is: - ANSWERS-2x
SSC at 37 degrees for 30 minutes
What would you do if you see high non-specific background on the
FISH slide just postwashed? - ANSWERS-Increase postwash
temperature
END OF
PAGE
1
, ASCP CG LATEST
CERTIFICATION EXAM
Which is preferred as a metaphase probe? - ANSWERS-SKY probe
A MLL FISH gave the following signal pattern 1F 1R 1G. What is the
ISCN of this cell? - ANSWERS-nuc ish (MLLx2)(5?MLL sep
3?MLLx1)
Fluorescence can be caused by what light source? - ANSWERS-
Mercury vapor lamps
To AVOID empty magnification, the total magnification seen by the eye
should: - ANSWERS-Not exceed 1000X the N.A. of the objective
What ARE functions of the CCD camera? - ANSWERS-Capturing light
Controlling the resolution of the image
Determination of matrix size based on the number of photo cells
How many bands are on chromosome 10 in the 350 to 400 band level
range? - ANSWERS-12 to 14
END OF
PAGE
2
, ASCP CG LATEST
CERTIFICATION EXAM
How many bands are on chromosome 10 in the 425 to 475 band level
range? - ANSWERS-15 to 21
How many bands are on chromosome 10 in the 500 to 550 band level
range? - ANSWERS-22 to 28
AML M0 type - ANSWERS-AML with minimal differentiation (no
chromosome change associated)
AML M1 type - ANSWERS-AML w/o differentiation (no associated
chrom changes either)
AML M2 type - ANSWERS-AML with differentiation
t(8;21)(q22;q22)
AML M3 type - ANSWERS-Acute Promyelocytic Leukemia (APL)
t(15;17)(q22;q11-12)
AML M4 type - ANSWERS-Acute Myelomonocytic Leukemia
(AMML)
END OF
PAGE
3
, ASCP CG LATEST
CERTIFICATION EXAM
inv(16)(p13q22)
AML M5 type - ANSWERS-Acute Monocytic Leukemia
t(9;11)(p22;q23)
other 11q23 rearrangements
AML M6 type - ANSWERS-Acute Erythroleukemia
del(5q), del(7q), +8
AML M7 type - ANSWERS-Acute Megakaryocytic leukemia
(no chrom abn associated)
What is the best agent to use against mycoplasma? - ANSWERS-BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWERS-comparing the new lot to a lot already in use
END OF
PAGE
4
CERTIFICATION EXAM
Which of the following ARE advantages of FISH diagnostics concerning
interphase testing? - ANSWERS-Can rapidly detect sex chromosomes or
aneuploidy of chromosomes.
Requires no culture
Requires only simple microscope analysis.
The temperature of -20oC is necessary in which of the following FISH
procedures? - ANSWERS-Probe storage
The temperature of 4oC is necessary in which of the following FISH
procedures? - ANSWERS-FITC-avidin storage
The temperature of 90oC is necessary in which of the following FISH
procedures? - ANSWERS-simultaneous denaturation
Most important pretreatment to age slides for FISH is: - ANSWERS-2x
SSC at 37 degrees for 30 minutes
What would you do if you see high non-specific background on the
FISH slide just postwashed? - ANSWERS-Increase postwash
temperature
END OF
PAGE
1
, ASCP CG LATEST
CERTIFICATION EXAM
Which is preferred as a metaphase probe? - ANSWERS-SKY probe
A MLL FISH gave the following signal pattern 1F 1R 1G. What is the
ISCN of this cell? - ANSWERS-nuc ish (MLLx2)(5?MLL sep
3?MLLx1)
Fluorescence can be caused by what light source? - ANSWERS-
Mercury vapor lamps
To AVOID empty magnification, the total magnification seen by the eye
should: - ANSWERS-Not exceed 1000X the N.A. of the objective
What ARE functions of the CCD camera? - ANSWERS-Capturing light
Controlling the resolution of the image
Determination of matrix size based on the number of photo cells
How many bands are on chromosome 10 in the 350 to 400 band level
range? - ANSWERS-12 to 14
END OF
PAGE
2
, ASCP CG LATEST
CERTIFICATION EXAM
How many bands are on chromosome 10 in the 425 to 475 band level
range? - ANSWERS-15 to 21
How many bands are on chromosome 10 in the 500 to 550 band level
range? - ANSWERS-22 to 28
AML M0 type - ANSWERS-AML with minimal differentiation (no
chromosome change associated)
AML M1 type - ANSWERS-AML w/o differentiation (no associated
chrom changes either)
AML M2 type - ANSWERS-AML with differentiation
t(8;21)(q22;q22)
AML M3 type - ANSWERS-Acute Promyelocytic Leukemia (APL)
t(15;17)(q22;q11-12)
AML M4 type - ANSWERS-Acute Myelomonocytic Leukemia
(AMML)
END OF
PAGE
3
, ASCP CG LATEST
CERTIFICATION EXAM
inv(16)(p13q22)
AML M5 type - ANSWERS-Acute Monocytic Leukemia
t(9;11)(p22;q23)
other 11q23 rearrangements
AML M6 type - ANSWERS-Acute Erythroleukemia
del(5q), del(7q), +8
AML M7 type - ANSWERS-Acute Megakaryocytic leukemia
(no chrom abn associated)
What is the best agent to use against mycoplasma? - ANSWERS-BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWERS-comparing the new lot to a lot already in use
END OF
PAGE
4
