UAMS Clinical Microbiology & Antimicrobial Susceptibility Exam 4: Gram Stains,
CSF Pathogens, LRT Specimen Quality, Abscess & Wound Culture Protocols,
Urinary Tract Microbiology, Stool Differential Media, Genital Smear Analysis,
Blood Culture Techniques, Beta-Lactam Resistance Mechanisms, MRSA
Detection, ESBL & Carbapenemase Testing, MIC & MBC Determinations,
McFarland Standards, Disk Diffusion, E-Test, Anaerobic vs Aerobic Pathogen
Identification, Protein & Cell Wall Synthesis Inhibitors, DNA/RNA Synthesis
Antimicrobials, Vancomycin Resistance, Plasmids & Transposons, Synergy vs
Antagonism, Intrinsic & Acquired Resistance, Susceptibility Profiles of Klebsiella,
Enterobacter, Stenotrophomonas, PBP2a, D-Test, VRE, Oxacillin & Cefoxitin
Screening, Beta-Lactamase Detection, Modified Hodge Test, Schlicter Serum
Bacterial Titer, Acridine Orange & Gram Stain Applications, Pathogen-Specific
Culture Media, and Clinical Specimen Handling Exam Questions Verified and
Provided with Complete A+ Graded Rationales Latest Updated 2026
Inappropriate gram stain tests
-Throat, vaginal or rectal for GC
-Throat for GAS
-No gram stain for stool specimens
-Only gram stain stool if looking for fecal leukocytes,
staph or campy
Acridine orange stain
,-Acridine orange stains nucleic acid of living and dead cells
-Bacteria and yeast
What plates do we subculture CSF?
What temperature and atmosphere is needed?
-BAP, CHOC, Thio
-37°C in CO2
What are common CSF pathogens?
-H. influenzae
-S. pneumoniae
-N. meningitidis
-GBS
-Listeria
-CNS
What is CSF rhinorrhea?
CSF leaking through nasal passage due to blunt trauma, skull fracture or a tumor
What plates do we subculture abscess's and wound cultures?
What temperature and atmosphere is needed?
-BAP, CHOC, MAC/EMB, CNA/PEA
, -anaerobic if requested
-37°C in CO2
**Pus is the best specimen**
What are common abscess and wound culture pathogens?
-Staph Aureus
-GAS
-Enterics
-Anaerobes
-PSAR
-Enterococci
What plates do we subculture stool?
What temperature and atmosphere is needed?
-BAP, MAC/EMB, HE/SS/XLD, GN broth
-37°C in air
Special stool culture plates
CampyBAP, CIN, TCBS, SMAC
What plates do we subculture genital specimens?
What temperature and atmosphere is needed?
CSF Pathogens, LRT Specimen Quality, Abscess & Wound Culture Protocols,
Urinary Tract Microbiology, Stool Differential Media, Genital Smear Analysis,
Blood Culture Techniques, Beta-Lactam Resistance Mechanisms, MRSA
Detection, ESBL & Carbapenemase Testing, MIC & MBC Determinations,
McFarland Standards, Disk Diffusion, E-Test, Anaerobic vs Aerobic Pathogen
Identification, Protein & Cell Wall Synthesis Inhibitors, DNA/RNA Synthesis
Antimicrobials, Vancomycin Resistance, Plasmids & Transposons, Synergy vs
Antagonism, Intrinsic & Acquired Resistance, Susceptibility Profiles of Klebsiella,
Enterobacter, Stenotrophomonas, PBP2a, D-Test, VRE, Oxacillin & Cefoxitin
Screening, Beta-Lactamase Detection, Modified Hodge Test, Schlicter Serum
Bacterial Titer, Acridine Orange & Gram Stain Applications, Pathogen-Specific
Culture Media, and Clinical Specimen Handling Exam Questions Verified and
Provided with Complete A+ Graded Rationales Latest Updated 2026
Inappropriate gram stain tests
-Throat, vaginal or rectal for GC
-Throat for GAS
-No gram stain for stool specimens
-Only gram stain stool if looking for fecal leukocytes,
staph or campy
Acridine orange stain
,-Acridine orange stains nucleic acid of living and dead cells
-Bacteria and yeast
What plates do we subculture CSF?
What temperature and atmosphere is needed?
-BAP, CHOC, Thio
-37°C in CO2
What are common CSF pathogens?
-H. influenzae
-S. pneumoniae
-N. meningitidis
-GBS
-Listeria
-CNS
What is CSF rhinorrhea?
CSF leaking through nasal passage due to blunt trauma, skull fracture or a tumor
What plates do we subculture abscess's and wound cultures?
What temperature and atmosphere is needed?
-BAP, CHOC, MAC/EMB, CNA/PEA
, -anaerobic if requested
-37°C in CO2
**Pus is the best specimen**
What are common abscess and wound culture pathogens?
-Staph Aureus
-GAS
-Enterics
-Anaerobes
-PSAR
-Enterococci
What plates do we subculture stool?
What temperature and atmosphere is needed?
-BAP, MAC/EMB, HE/SS/XLD, GN broth
-37°C in air
Special stool culture plates
CampyBAP, CIN, TCBS, SMAC
What plates do we subculture genital specimens?
What temperature and atmosphere is needed?
