CSMLS EXAM PREP QUESTIONS AND
ANSWERS
Cryostat temperature - Correct Answers --18 to -20C
Microtome cuts - Correct Answers -4-6 um
Paraffin Bath Temperature - Correct Answers -45C
Spleen functions - Correct Answers -destroy old red blood cells, produce antibodies,
store red blood cells, filter microorganism
Standard Precautions - Correct Answers -a set of infection control practices used to
prevent transmission of diseases that can be acquired by contact with blood, body
fluids, non-intact skin (including rashes), and mucous membranes.
Tourniquet cleaned with - Correct Answers -70-90% ethyl alcohol (antiseptic)
Skin cleaned with - Correct Answers -70-90% ethyl alcohol
Lab bench Cleaned with - Correct Answers -1% bleach
Metal surfaces cleaned with - Correct Answers -2% gluteraldehyde
Modes of entry - Correct Answers -inhalation, ingestion, absorption, injection
Antiseptics - Correct Answers -Solutions that are applied directly to the skin and prevent
or slow the growth of pathogens.
H2O2, iodine, 1-2% phenol, 70% alcohols
Disinfectants - Correct Answers -used on inanimate objects
Sodium hypochlorite, 1% bleach
Gluteraldehyde - Correct Answers -A chemical fixative often used in the preparation of
tissue for electron microscopy used on metals
2% solutions
store in dark
Autoclave - Correct Answers -15 min or more, 121C, 103.4 KPA (15psi)
, Plasma - Correct Answers -contains fibrinogen, for coag tests
3 layers (RBCS, WBCS, plasma)
Serum - Correct Answers -no fibrinogen, forms clot, clot contracts expressses serum.
For chem serology & blood bank
2 layers blood clot and serum
Do we perform differentials on buffy coat - Correct Answers -no
What anticoagulant is used for transfusion - Correct Answers -CPDA-AS (citrate
phosphate dextrose adenosine1)
Forming a clot - Correct Answers -Plts form primary plug, coag factors activated, coag
begins and end product fibrinogen is converted to fibrin to forma mesh network (clot)
Defibrination - Correct Answers -use beads/rods and rotate blood in a glass container
then centrifuge serum and cells
for people with low WBCS because higher WBC concentration
Intrinsic pathway of coagulation - Correct Answers -in vascular system, aPTT
Intrinsic Factors - Correct Answers -f 12, f 11, (CA), f 9, PLT f3, f 8
extrinsic pathway of coagulation - Correct Answers -external trauma/ tissue injury
PT test
REnal screening TEsts - Correct Answers -BUN, serum creatinine, serum uric acid,
other (CA,P,Na,K,A/g ratio)
urinary constituents - Correct Answers -most common: Na, K, Cl, Ca, P
Na - urinary constituent - Correct Answers -maintains acid-base balance
regulator of bodies ability to retain water
K- urinary constituent - Correct Answers -bodys buffering system, maintains electrolyte
balance in cellular fluids
Extrinsic factors - Correct Answers -tissue thromboplastin, CA, F7
Common Pathway of coagulation - Correct Answers -Factor X, (CA+ PLT F3), Factor V,
PT -> thrombin, fibrinogen -> fibrin, CLOT
EDTA - Correct Answers -ethylene diamine tetra acetic acid
chelates CA
preserves cell
ANSWERS
Cryostat temperature - Correct Answers --18 to -20C
Microtome cuts - Correct Answers -4-6 um
Paraffin Bath Temperature - Correct Answers -45C
Spleen functions - Correct Answers -destroy old red blood cells, produce antibodies,
store red blood cells, filter microorganism
Standard Precautions - Correct Answers -a set of infection control practices used to
prevent transmission of diseases that can be acquired by contact with blood, body
fluids, non-intact skin (including rashes), and mucous membranes.
Tourniquet cleaned with - Correct Answers -70-90% ethyl alcohol (antiseptic)
Skin cleaned with - Correct Answers -70-90% ethyl alcohol
Lab bench Cleaned with - Correct Answers -1% bleach
Metal surfaces cleaned with - Correct Answers -2% gluteraldehyde
Modes of entry - Correct Answers -inhalation, ingestion, absorption, injection
Antiseptics - Correct Answers -Solutions that are applied directly to the skin and prevent
or slow the growth of pathogens.
H2O2, iodine, 1-2% phenol, 70% alcohols
Disinfectants - Correct Answers -used on inanimate objects
Sodium hypochlorite, 1% bleach
Gluteraldehyde - Correct Answers -A chemical fixative often used in the preparation of
tissue for electron microscopy used on metals
2% solutions
store in dark
Autoclave - Correct Answers -15 min or more, 121C, 103.4 KPA (15psi)
, Plasma - Correct Answers -contains fibrinogen, for coag tests
3 layers (RBCS, WBCS, plasma)
Serum - Correct Answers -no fibrinogen, forms clot, clot contracts expressses serum.
For chem serology & blood bank
2 layers blood clot and serum
Do we perform differentials on buffy coat - Correct Answers -no
What anticoagulant is used for transfusion - Correct Answers -CPDA-AS (citrate
phosphate dextrose adenosine1)
Forming a clot - Correct Answers -Plts form primary plug, coag factors activated, coag
begins and end product fibrinogen is converted to fibrin to forma mesh network (clot)
Defibrination - Correct Answers -use beads/rods and rotate blood in a glass container
then centrifuge serum and cells
for people with low WBCS because higher WBC concentration
Intrinsic pathway of coagulation - Correct Answers -in vascular system, aPTT
Intrinsic Factors - Correct Answers -f 12, f 11, (CA), f 9, PLT f3, f 8
extrinsic pathway of coagulation - Correct Answers -external trauma/ tissue injury
PT test
REnal screening TEsts - Correct Answers -BUN, serum creatinine, serum uric acid,
other (CA,P,Na,K,A/g ratio)
urinary constituents - Correct Answers -most common: Na, K, Cl, Ca, P
Na - urinary constituent - Correct Answers -maintains acid-base balance
regulator of bodies ability to retain water
K- urinary constituent - Correct Answers -bodys buffering system, maintains electrolyte
balance in cellular fluids
Extrinsic factors - Correct Answers -tissue thromboplastin, CA, F7
Common Pathway of coagulation - Correct Answers -Factor X, (CA+ PLT F3), Factor V,
PT -> thrombin, fibrinogen -> fibrin, CLOT
EDTA - Correct Answers -ethylene diamine tetra acetic acid
chelates CA
preserves cell
