CSMLS GENERAL EXAM QUESTIONS
WITH CORRECT ANSWERS
Iodophor - Correct Answers -Combination of iodine and a solubilizing agent or carrier
that liberates free iodine in solution; a chemical disinfectant.
Carrier molecule is alcohol, carrier releases iodine slowly.
Procedural Design - Correct Answers -Ensures safety in the performance of the
procedure or in the operation of equipment.
Use of PPE is also procedural control.
If a blood specimen is spilled on a lab bench the first step in clean up should be -
Correct Answers -Blood must be absorbed before applying disinfectant because
1. Blood and other organic material inactivate disinfectants
2. Prevent splashing
Anticoagulant of choice for Hematology smears - Correct Answers -Tripotassium EDTA
How long may a specimen be used for compatibility testing, if the patient's history is
unknown - Correct Answers -3 Days
Serum specimen left uncovered - Correct Answers -Will evaporate and produce falsely
elevated values for most analytes except gas.
Collected Blood specimen glucose utilization - Correct Answers -7% glucose per hour
Heparin antagonist - Correct Answers -protamine sulfate
Whooping cough Specimen - Correct Answers -Nasopharyngeal area
B. pertusiss
Order of Draw - Correct Answers -Blood culture
Blue tube for coagulation (Sodium Citrate)
Red No Gel
, Gold SST (Plain tube w/gel and clot activator additive)
Green and Dark Green (Heparin, with and without gel)
Lavender (EDTA)
Pink - Blood Bank (EDTA)
Gray (Oxalate/Fluoride)
Bacteriostatic agent added to most antisera - Correct Answers -0.1% sodium azide
In the preparation of an accurate reagent, which item is the most useful - Correct
Answers -Volumetric flask
Prozone effect - Correct Answers -An excess of antibody molecules will saturate all the
antigenic sites not enabling cross linking to occur and would appear as a false negative.
Which IgG subclasses of Rh antibodies are most significant? - Correct Answers -IgG 1
and 3
Under what principle is an employer held accountable for the actions of his/her
employees at work - Correct Answers -vicarious liability
Secretor - Correct Answers -lewis (a-b+)
Non-secretor - Correct Answers -Lewis (a+b-)
Coombs control cells - Correct Answers -RBCs w/ antibodies already attached to them,
used as a control for negative reactions
Added to negative DAT and IAT reactions
MUST BE + TO BE VALID
Cord cells - Correct Answers -anti-i positive
During protein electrophoresis, with a buffer pH of 8.6, the gamma globulins appear to
have migrated towards the cathode. What is the most likely reason for this - Correct
Answers -Endosomosis
The gamma globulins are dragged behind the application point, with the solvent flow
because they are the least net negative proteins at a pH of 8.6
A procedure requires you to read the test solution at 510 nm and you set the
wavelength at 610 nm. What affect will this have on your readings? - Correct Answers -
the wavelength 610 is 100nm away from the desired setting where the maximum
absorbance occurs for the specific reaction.
WITH CORRECT ANSWERS
Iodophor - Correct Answers -Combination of iodine and a solubilizing agent or carrier
that liberates free iodine in solution; a chemical disinfectant.
Carrier molecule is alcohol, carrier releases iodine slowly.
Procedural Design - Correct Answers -Ensures safety in the performance of the
procedure or in the operation of equipment.
Use of PPE is also procedural control.
If a blood specimen is spilled on a lab bench the first step in clean up should be -
Correct Answers -Blood must be absorbed before applying disinfectant because
1. Blood and other organic material inactivate disinfectants
2. Prevent splashing
Anticoagulant of choice for Hematology smears - Correct Answers -Tripotassium EDTA
How long may a specimen be used for compatibility testing, if the patient's history is
unknown - Correct Answers -3 Days
Serum specimen left uncovered - Correct Answers -Will evaporate and produce falsely
elevated values for most analytes except gas.
Collected Blood specimen glucose utilization - Correct Answers -7% glucose per hour
Heparin antagonist - Correct Answers -protamine sulfate
Whooping cough Specimen - Correct Answers -Nasopharyngeal area
B. pertusiss
Order of Draw - Correct Answers -Blood culture
Blue tube for coagulation (Sodium Citrate)
Red No Gel
, Gold SST (Plain tube w/gel and clot activator additive)
Green and Dark Green (Heparin, with and without gel)
Lavender (EDTA)
Pink - Blood Bank (EDTA)
Gray (Oxalate/Fluoride)
Bacteriostatic agent added to most antisera - Correct Answers -0.1% sodium azide
In the preparation of an accurate reagent, which item is the most useful - Correct
Answers -Volumetric flask
Prozone effect - Correct Answers -An excess of antibody molecules will saturate all the
antigenic sites not enabling cross linking to occur and would appear as a false negative.
Which IgG subclasses of Rh antibodies are most significant? - Correct Answers -IgG 1
and 3
Under what principle is an employer held accountable for the actions of his/her
employees at work - Correct Answers -vicarious liability
Secretor - Correct Answers -lewis (a-b+)
Non-secretor - Correct Answers -Lewis (a+b-)
Coombs control cells - Correct Answers -RBCs w/ antibodies already attached to them,
used as a control for negative reactions
Added to negative DAT and IAT reactions
MUST BE + TO BE VALID
Cord cells - Correct Answers -anti-i positive
During protein electrophoresis, with a buffer pH of 8.6, the gamma globulins appear to
have migrated towards the cathode. What is the most likely reason for this - Correct
Answers -Endosomosis
The gamma globulins are dragged behind the application point, with the solvent flow
because they are the least net negative proteins at a pH of 8.6
A procedure requires you to read the test solution at 510 nm and you set the
wavelength at 610 nm. What affect will this have on your readings? - Correct Answers -
the wavelength 610 is 100nm away from the desired setting where the maximum
absorbance occurs for the specific reaction.
