Cryostat temperature - Correct Answer -18 to -20C
Microtome cuts - Correct Answer 4-6 um
Paraffin Bath Temperature - Correct Answer 45C
Spleen functions - Correct Answer destroy old red blood cells, produce antibodies, store red blood cells,
filter microorganism
Standard Precautions - Correct Answer a set of infection control practices used to prevent transmission
of diseases that can be acquired by contact with blood, body fluids, non-intact skin (including rashes),
and mucous membranes.
Tourniquet cleaned with - Correct Answer 70-90% ethyl alcohol (antiseptic)
Skin cleaned with - Correct Answer 70-90% ethyl alcohol
Lab bench Cleaned with - Correct Answer 1% bleach
Metal surfaces cleaned with - Correct Answer 2% gluteraldehyde
Modes of entry - Correct Answer inhalation, ingestion, absorption, injection
Antiseptics - Correct Answer Solutions that are applied directly to the skin and prevent or slow the
growth of pathogens.
H2O2, iodine, 1-2% phenol, 70% alcohols
,Disinfectants - Correct Answer used on inanimate objects
Sodium hypochlorite, 1% bleach
Gluteraldehyde - Correct Answer A chemical fixative often used in the preparation of tissue for electron
microscopy used on metals
2% solutions
store in dark
Autoclave - Correct Answer 15 min or more, 121C, 103.4 KPA (15psi)
Plasma - Correct Answer contains fibrinogen, for coag tests
3 layers (RBCS, WBCS, plasma)
Serum - Correct Answer no fibrinogen, forms clot, clot contracts expressses serum. For chem serology &
blood bank
2 layers blood clot and serum
Do we perform differentials on buffy coat - Correct Answer no
What anticoagulant is used for transfusion - Correct Answer CPDA-AS (citrate phosphate dextrose
adenosine1)
Forming a clot - Correct Answer Plts form primary plug, coag factors activated, coag begins and end
product fibrinogen is converted to fibrin to forma mesh network (clot)
Defibrination - Correct Answer use beads/rods and rotate blood in a glass container then centrifuge
serum and cells
for people with low WBCS because higher WBC concentration
Intrinsic pathway of coagulation - Correct Answer in vascular system, aPTT
, Intrinsic Factors - Correct Answer f 12, f 11, (CA), f 9, PLT f3, f 8
extrinsic pathway of coagulation - Correct Answer external trauma/ tissue injury
PT test
Extrinsic factors - Correct Answer tissue thromboplastin, CA, F7
Common Pathway of coagulation - Correct Answer Factor X, (CA+ PLT F3), Factor V, PT -> thrombin,
fibrinogen -> fibrin, CLOT
EDTA - Correct Answer ethylene diamine tetra acetic acid
chelates CA
preserves cell
Na Citrate - Correct Answer chelates CA, 3.2% preferred more gentle,
Heparin - Correct Answer hemo - osmotic fragility, LAP score
chem - VBGS, Glucose, electrolytes
ANTI THROMBIN - activates fibrinogen
NaF antienolase - Correct Answer prevents glycolysis, good for glucose for electrolytes
not anticoagulant
K oxalate anticoagulant - Correct Answer chelates CA
ACD/CPD - Correct Answer donation bags, chelates CA, RBCS functions up to 42 days with addition of
nutricell
(acid citrate dextrose/citrate phosphate dextrose_
Microtome cuts - Correct Answer 4-6 um
Paraffin Bath Temperature - Correct Answer 45C
Spleen functions - Correct Answer destroy old red blood cells, produce antibodies, store red blood cells,
filter microorganism
Standard Precautions - Correct Answer a set of infection control practices used to prevent transmission
of diseases that can be acquired by contact with blood, body fluids, non-intact skin (including rashes),
and mucous membranes.
Tourniquet cleaned with - Correct Answer 70-90% ethyl alcohol (antiseptic)
Skin cleaned with - Correct Answer 70-90% ethyl alcohol
Lab bench Cleaned with - Correct Answer 1% bleach
Metal surfaces cleaned with - Correct Answer 2% gluteraldehyde
Modes of entry - Correct Answer inhalation, ingestion, absorption, injection
Antiseptics - Correct Answer Solutions that are applied directly to the skin and prevent or slow the
growth of pathogens.
H2O2, iodine, 1-2% phenol, 70% alcohols
,Disinfectants - Correct Answer used on inanimate objects
Sodium hypochlorite, 1% bleach
Gluteraldehyde - Correct Answer A chemical fixative often used in the preparation of tissue for electron
microscopy used on metals
2% solutions
store in dark
Autoclave - Correct Answer 15 min or more, 121C, 103.4 KPA (15psi)
Plasma - Correct Answer contains fibrinogen, for coag tests
3 layers (RBCS, WBCS, plasma)
Serum - Correct Answer no fibrinogen, forms clot, clot contracts expressses serum. For chem serology &
blood bank
2 layers blood clot and serum
Do we perform differentials on buffy coat - Correct Answer no
What anticoagulant is used for transfusion - Correct Answer CPDA-AS (citrate phosphate dextrose
adenosine1)
Forming a clot - Correct Answer Plts form primary plug, coag factors activated, coag begins and end
product fibrinogen is converted to fibrin to forma mesh network (clot)
Defibrination - Correct Answer use beads/rods and rotate blood in a glass container then centrifuge
serum and cells
for people with low WBCS because higher WBC concentration
Intrinsic pathway of coagulation - Correct Answer in vascular system, aPTT
, Intrinsic Factors - Correct Answer f 12, f 11, (CA), f 9, PLT f3, f 8
extrinsic pathway of coagulation - Correct Answer external trauma/ tissue injury
PT test
Extrinsic factors - Correct Answer tissue thromboplastin, CA, F7
Common Pathway of coagulation - Correct Answer Factor X, (CA+ PLT F3), Factor V, PT -> thrombin,
fibrinogen -> fibrin, CLOT
EDTA - Correct Answer ethylene diamine tetra acetic acid
chelates CA
preserves cell
Na Citrate - Correct Answer chelates CA, 3.2% preferred more gentle,
Heparin - Correct Answer hemo - osmotic fragility, LAP score
chem - VBGS, Glucose, electrolytes
ANTI THROMBIN - activates fibrinogen
NaF antienolase - Correct Answer prevents glycolysis, good for glucose for electrolytes
not anticoagulant
K oxalate anticoagulant - Correct Answer chelates CA
ACD/CPD - Correct Answer donation bags, chelates CA, RBCS functions up to 42 days with addition of
nutricell
(acid citrate dextrose/citrate phosphate dextrose_
