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CHEM 301/ CHEM301 Final Exam V2 | Biochemistry – 2026/2027 Academic Year – Questions & Answers

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CHEM 301/ CHEM301 Final Exam V2 | Biochemistry – 2026/2027 Academic Year – Questions & Answers

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CHEM 301/ CHEM301
Final Exam V2 |
Biochemistry – 2026/2027
Academic Year –
Questions & Answers




muscle twitches, muscles weakness, and degradation of nerve cells - ANSWER -An individual with the
MERRF disease tends to have symptoms of



inner membrane - ANSWER -In mitochondria, the molecule that produces ATP is located in the



rotation of ATP synthase - ANSWER -What process results in ATP formation?



F and F2 - ANSWER -Which of the following is not a part of ATP synthase?

, -F

- Fo

- F1

- F2



3 - ANSWER -Oxidative phosphorylation occurs in ___ phases.



CAC - ANSWER -Oxidative phosphorylation uses NADH and FADH2 generated from the ___.



synthesis of ATP from ADP + Pi - ANSWER -Oxidative phosphorylation is the synthesis of ___ from
___.



ETC - ANSWER -Oxidative phosphorylation uses energy generated by the ___ reactions.



After the first phase, the chemical reactions of the ETC provide energy from NADH and FADH2 to
pump H+ ions from the mitochondrial matrix into the inter membrane space. These reactions also
provide electrons and H+ to convert oxygen into water. Together, these reactions create a H+
gradient that allows H+ to flow through and rotate the ATP synthase in order to produce ATP. -
ANSWER -Oxidative phosphorylation occurs in 3 phases. The first phase is the flow of electrons from
NADH and FADH2. What happens after this first phase is complete?



A. The mitochondrion is the main site of oxidative phosphorylation, which can be attributed to its
two-membrane structure.

- The outer membrane is a bilayer that contains porin proteins, which allow ions and molecules to
cross into the intermembrane space. The intermembrane space is located between the inner and
outer membrane and contains solution.

- The inner membrane is also a bilayer but is highly selective, allowing only select ions and molecules
to cross. This selective characteristic of the inner membrane is critical for maintaining the specific
concentrations of H+, ATP, and other species within the mitochondrial matrix.

- The matrix is the innermost space of the mitochondrion and is where the citric acid cycle as well as
parts of beta oxidation and the urea cycle occur. This close proximity of reaction cycles is efficient
because it allows NADH and FADH2, produced by the citric acid cycle, to also partici - ANSWER -
Describe the critical structure of the mitochondrion that are important for the production of ATP.



A. What structural features of the mitochondrion support ATP formation.

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