micro 290 lab final
1. indicate three general - physical (heat, radiation like UV rays)
methods you can kill - biological (antibiotic & phage)
germs & example - chemical (alcohol & bleach)
2. which structure of mi- DNA
crobes are mostly dam-
aged by UV light?
3. what type of lesion UV thymine dimers which lead to mutation in DNA
makes in the DNA of bac-
teria and what does it lead
to?
4. what is the most lethal UV-C; 100-290 nm wavelength
form of UV light and what
is its wavelength?
5. indicate the wavelength of 254 nm used for sterilization of air, surfaces, water, & operating rooms
UV light emitted by germi-
cidal lamp. give examples
of type of material that
these lamps are common-
ly used for to kill germs
(sterilization)
6. what happened to the because endospores of bacteria are very resistant to many harsh
bacteria when they were conditions including heat, dryness, & UV light
frozen? explain the out-
come and reason for each
type of bacteria after they
were irritated, heated, &
dried.
, micro 290 lab final
7. definition of antibiotics a type of antimicrobial agent that is made by a microorganism and
kills or inhibit other microorganisms
8. what does efficacy of an it means how effectively the antibiotics is killing or inhibiting the
antibiotic mean and how is microbe of our interest. it is tested by the kirby-bauer method which
it measured? tests the sensitivity of bacteria to antibiotics
9. what is the definition of if antibiotics inhibits the growth of the organism, a clear circular area
'zone of inhibition' and appears around the disk. the larger the diameter of the zone of inhi-
how does it relate to the bition, the more effective the antibiotic is in killing the organism and
sensitivity of the microbes a better choice for use in patients that are suffering from infections
to the tested antibiotic? by that organism
10. which antibiotics has the C (largest clear ring) & B (smallest clear ring)
greatest zone of inhibition
and which one has the
least?
11. which would be your first C (largest clear ring)
choice for treating your
patient?
12. if C is penicillin, and D (second largest clear ring)
your patient is allergic to
penicillin, which antibiotic
would you use?
13. which antibiotic would you B (smallest clear ring)
not use?
14. what type of agar is com- mueller-hinton agar
monly used for the kir-
by-bauer test?
1. indicate three general - physical (heat, radiation like UV rays)
methods you can kill - biological (antibiotic & phage)
germs & example - chemical (alcohol & bleach)
2. which structure of mi- DNA
crobes are mostly dam-
aged by UV light?
3. what type of lesion UV thymine dimers which lead to mutation in DNA
makes in the DNA of bac-
teria and what does it lead
to?
4. what is the most lethal UV-C; 100-290 nm wavelength
form of UV light and what
is its wavelength?
5. indicate the wavelength of 254 nm used for sterilization of air, surfaces, water, & operating rooms
UV light emitted by germi-
cidal lamp. give examples
of type of material that
these lamps are common-
ly used for to kill germs
(sterilization)
6. what happened to the because endospores of bacteria are very resistant to many harsh
bacteria when they were conditions including heat, dryness, & UV light
frozen? explain the out-
come and reason for each
type of bacteria after they
were irritated, heated, &
dried.
, micro 290 lab final
7. definition of antibiotics a type of antimicrobial agent that is made by a microorganism and
kills or inhibit other microorganisms
8. what does efficacy of an it means how effectively the antibiotics is killing or inhibiting the
antibiotic mean and how is microbe of our interest. it is tested by the kirby-bauer method which
it measured? tests the sensitivity of bacteria to antibiotics
9. what is the definition of if antibiotics inhibits the growth of the organism, a clear circular area
'zone of inhibition' and appears around the disk. the larger the diameter of the zone of inhi-
how does it relate to the bition, the more effective the antibiotic is in killing the organism and
sensitivity of the microbes a better choice for use in patients that are suffering from infections
to the tested antibiotic? by that organism
10. which antibiotics has the C (largest clear ring) & B (smallest clear ring)
greatest zone of inhibition
and which one has the
least?
11. which would be your first C (largest clear ring)
choice for treating your
patient?
12. if C is penicillin, and D (second largest clear ring)
your patient is allergic to
penicillin, which antibiotic
would you use?
13. which antibiotic would you B (smallest clear ring)
not use?
14. what type of agar is com- mueller-hinton agar
monly used for the kir-
by-bauer test?
