MB (ASCP) Exam Updated Questions
And Answers With 100% Correct
Answers Graded A+
QUESTIONS AND ANSWERS
Predominant form of DNA. ANSWER - Right-handed B
DNA and histone interaction is due to what type of bond?. ANSWER - Ionic
What stains to the minor groove of DNA duplex?. ANSWER - SYBR Green
What is the most abundant type of RNA?. ANSWER - Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus?. ANSWER - Genome and mRNA
What increases half-life of mRNA?. ANSWER - 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active?. ANSWER - Euchromatin
What is the most common type of eukaryotic DNA?. ANSWER - Satellite DNA
What specimen has the most DNA according to molecular weight?. ANSWER - White
Blood Cells
What amino acids compose histones?. ANSWER - Lysine and Arginine
Amino acid that all proteins start with:. ANSWER - Methionine
What protects the DNA strand during replication?. ANSWER - Single-stranded binding
protein
Leading Strand. ANSWER - 3' to 5' template
, Lagging Strand. ANSWER - 5' to 3' template
What DNA strand is being copied in transcription?. ANSWER - Antisense Strand
First step after newly formed mRNA is made?. ANSWER - Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme?.
ANSWER - Telomerase
What polymerase has repair activity?. ANSWER - Beta/Epsilon
What polymerase acts on leading strand?. ANSWER - Delta
What polymerase acts on lagging strand?. ANSWER - Alpha
Which polymerase is used for mitochondrial replication and repair?. ANSWER - Gamma
What polymerase binds the 5' end to 3' end of dsDNA?. ANSWER - Topoisomerase
Which RNA polymerase is mainly for transcription?. ANSWER - RNA Pol II
Which polymerase is involved with mRNA synthesis?. ANSWER - RNA Pol II
What inactivates RNAses?. ANSWER - Diethyl pyrocarbonate (DEPC) or bake glassware
for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites?. ANSWER - Restriction
Enzyme Type II
What type of restriction enzyme lacks methylation activity?. ANSWER - Restriction
Enzyme Type II
What is necessary to make cDNA?. ANSWER - Reverse transcriptase
And Answers With 100% Correct
Answers Graded A+
QUESTIONS AND ANSWERS
Predominant form of DNA. ANSWER - Right-handed B
DNA and histone interaction is due to what type of bond?. ANSWER - Ionic
What stains to the minor groove of DNA duplex?. ANSWER - SYBR Green
What is the most abundant type of RNA?. ANSWER - Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus?. ANSWER - Genome and mRNA
What increases half-life of mRNA?. ANSWER - 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active?. ANSWER - Euchromatin
What is the most common type of eukaryotic DNA?. ANSWER - Satellite DNA
What specimen has the most DNA according to molecular weight?. ANSWER - White
Blood Cells
What amino acids compose histones?. ANSWER - Lysine and Arginine
Amino acid that all proteins start with:. ANSWER - Methionine
What protects the DNA strand during replication?. ANSWER - Single-stranded binding
protein
Leading Strand. ANSWER - 3' to 5' template
, Lagging Strand. ANSWER - 5' to 3' template
What DNA strand is being copied in transcription?. ANSWER - Antisense Strand
First step after newly formed mRNA is made?. ANSWER - Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme?.
ANSWER - Telomerase
What polymerase has repair activity?. ANSWER - Beta/Epsilon
What polymerase acts on leading strand?. ANSWER - Delta
What polymerase acts on lagging strand?. ANSWER - Alpha
Which polymerase is used for mitochondrial replication and repair?. ANSWER - Gamma
What polymerase binds the 5' end to 3' end of dsDNA?. ANSWER - Topoisomerase
Which RNA polymerase is mainly for transcription?. ANSWER - RNA Pol II
Which polymerase is involved with mRNA synthesis?. ANSWER - RNA Pol II
What inactivates RNAses?. ANSWER - Diethyl pyrocarbonate (DEPC) or bake glassware
for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites?. ANSWER - Restriction
Enzyme Type II
What type of restriction enzyme lacks methylation activity?. ANSWER - Restriction
Enzyme Type II
What is necessary to make cDNA?. ANSWER - Reverse transcriptase
