MB ASCP EXAM 2026 COMPREHENSIVE
STUDY GUIDE QUESTIONS AND FULL
SOLUTION
◉ Inhibitors of PCR Answer: Hemoglobin, Heparin, Xylene, Phenol,
Cyanol, and Bromophenol Blue
◉ Order to add to a PCR reaction: Answer: Master mix > patient
sample > positive control > negative control
◉ Pre Area Answer: Positive Pressure
◉ Post Area Answer: Negative Pressure
◉ Which PCR requires two steps for specificity? Answer: Nested PCR
◉ Which microorganism is detected by branched DNA
amplification? Answer: DNA or RNA
◉ Which technique uses RNA, antibodies, and DNA? Answer: Hybrid
Capture Assay
,◉ Use of bisulfite treatment of DNA to determine its pattern of
methylation Answer: Bisulfite DNA
◉ What tests are isothermal? Answer: NASBA, TMA, & SDA
◉ Which leukemia is associated with FLT3 gene? Answer: AML
◉ T-cell gene rearrangement is seen in what malignancy? Answer:
Sezary Syndrome
◉ Which translocation causes Acute Premyelocytic Leukemia?
Answer: t 15;17
◉ Ewing Sarcoma Answer: t 11;22
◉ Synovial Sarcoma Answer: t X;18
◉ Follicular Lymphoma Answer: t 14;18
◉ What genes would be screened in a colorectal panel? Answer:
MSH2 & MLH1
, ◉ What gene mutation causes a high risk of breast or ovarian
cancer? Answer: BRCA1 and BRCA2
◉ What does MSI test for? Answer: Mutations in mismatch repair
genes (MMR)
◉ What disease is related to factor V coagulation gene (F5 1q23)?
Answer: Thrombossis
◉ How many copies are in a full Fragile X mutation? Answer: 200-
2000+ CGG repeats
◉ What disease is known for numerous mutations and large
deletions? Answer: Muscular Dystrophy (Duchenne)
◉ What HPV causes cervical cancer? Answer: 16 & 18
◉ You receive a paraffin embedded specimen for HPV typing, which
method should you use? Answer: PCR
◉ Significant change in HIV viral load: Answer: 0.5 log10
STUDY GUIDE QUESTIONS AND FULL
SOLUTION
◉ Inhibitors of PCR Answer: Hemoglobin, Heparin, Xylene, Phenol,
Cyanol, and Bromophenol Blue
◉ Order to add to a PCR reaction: Answer: Master mix > patient
sample > positive control > negative control
◉ Pre Area Answer: Positive Pressure
◉ Post Area Answer: Negative Pressure
◉ Which PCR requires two steps for specificity? Answer: Nested PCR
◉ Which microorganism is detected by branched DNA
amplification? Answer: DNA or RNA
◉ Which technique uses RNA, antibodies, and DNA? Answer: Hybrid
Capture Assay
,◉ Use of bisulfite treatment of DNA to determine its pattern of
methylation Answer: Bisulfite DNA
◉ What tests are isothermal? Answer: NASBA, TMA, & SDA
◉ Which leukemia is associated with FLT3 gene? Answer: AML
◉ T-cell gene rearrangement is seen in what malignancy? Answer:
Sezary Syndrome
◉ Which translocation causes Acute Premyelocytic Leukemia?
Answer: t 15;17
◉ Ewing Sarcoma Answer: t 11;22
◉ Synovial Sarcoma Answer: t X;18
◉ Follicular Lymphoma Answer: t 14;18
◉ What genes would be screened in a colorectal panel? Answer:
MSH2 & MLH1
, ◉ What gene mutation causes a high risk of breast or ovarian
cancer? Answer: BRCA1 and BRCA2
◉ What does MSI test for? Answer: Mutations in mismatch repair
genes (MMR)
◉ What disease is related to factor V coagulation gene (F5 1q23)?
Answer: Thrombossis
◉ How many copies are in a full Fragile X mutation? Answer: 200-
2000+ CGG repeats
◉ What disease is known for numerous mutations and large
deletions? Answer: Muscular Dystrophy (Duchenne)
◉ What HPV causes cervical cancer? Answer: 16 & 18
◉ You receive a paraffin embedded specimen for HPV typing, which
method should you use? Answer: PCR
◉ Significant change in HIV viral load: Answer: 0.5 log10
