MB ASCP EXAM 2026 FINAL REVIEW SET
COMPLETE QUESTIONS AND ANSWERS
◉ How does agarose gel pore size relate to concentration? Answer:
Inversely proportional
◉ What is denatured during a southern blot? Answer: Hydrogen
bonds (inter-strand bonds)
◉ Melting temperature formula Answer: Tm = (4C x GC Pairs) + (2C
x AT pairs)
◉ Which is likely to interfere with fluorescent probe? Answer: 5' G
◉ Proper GC content of primer Answer: 35-65%
◉ Inhibitors of PCR Answer: Hemoglobin, Heparin, Xylene, Phenol,
Cyanol, and Bromophenol Blue
◉ Order to add to a PCR reaction: Answer: Master mix > patient
sample > positive control > negative control
,◉ Pre Area Answer: Positive Pressure
◉ Post Area Answer: Negative Pressure
◉ Which PCR requires two steps for specificity? Answer: Nested PCR
◉ Which microorganism is detected by branched DNA
amplification? Answer: DNA or RNA
◉ Which technique uses RNA, antibodies, and DNA? Answer: Hybrid
Capture Assay
◉ Use of bisulfite treatment of DNA to determine its pattern of
methylation Answer: Bisulfite DNA
◉ What tests are isothermal? Answer: NASBA, TMA, & SDA
◉ Which leukemia is associated with FLT3 gene? Answer: AML
◉ T-cell gene rearrangement is seen in what malignancy? Answer:
Sezary Syndrome
,◉ Which translocation causes Acute Premyelocytic Leukemia?
Answer: t 15;17
◉ Ewing Sarcoma Answer: t 11;22
◉ Synovial Sarcoma Answer: t X;18
◉ Follicular Lymphoma Answer: t 14;18
◉ What genes would be screened in a colorectal panel? Answer:
MSH2 & MLH1
◉ What gene mutation causes a high risk of breast or ovarian
cancer? Answer: BRCA1 and BRCA2
◉ What does MSI test for? Answer: Mutations in mismatch repair
genes (MMR)
◉ What disease is related to factor V coagulation gene (F5 1q23)?
Answer: Thrombossis
◉ How many copies are in a full Fragile X mutation? Answer: 200-
2000+ CGG repeats
, ◉ What disease is known for numerous mutations and large
deletions? Answer: Muscular Dystrophy (Duchenne)
◉ What HPV causes cervical cancer? Answer: 16 & 18
◉ You receive a paraffin embedded specimen for HPV typing, which
method should you use? Answer: PCR
◉ Significant change in HIV viral load: Answer: 0.5 log10
◉ Lowest viral concentration that can be detected over background
or a negative control in the test assay Answer: Limit of Detection
(LOD)
◉ HCV consensus sequence: Answer: G-rich Core Gene
◉ What responds poorly to ribavarin/interferon treatment? Answer:
HCV Type 1B
◉ What virus affects patients after kidney transplant? Answer: CMV
or BKV
COMPLETE QUESTIONS AND ANSWERS
◉ How does agarose gel pore size relate to concentration? Answer:
Inversely proportional
◉ What is denatured during a southern blot? Answer: Hydrogen
bonds (inter-strand bonds)
◉ Melting temperature formula Answer: Tm = (4C x GC Pairs) + (2C
x AT pairs)
◉ Which is likely to interfere with fluorescent probe? Answer: 5' G
◉ Proper GC content of primer Answer: 35-65%
◉ Inhibitors of PCR Answer: Hemoglobin, Heparin, Xylene, Phenol,
Cyanol, and Bromophenol Blue
◉ Order to add to a PCR reaction: Answer: Master mix > patient
sample > positive control > negative control
,◉ Pre Area Answer: Positive Pressure
◉ Post Area Answer: Negative Pressure
◉ Which PCR requires two steps for specificity? Answer: Nested PCR
◉ Which microorganism is detected by branched DNA
amplification? Answer: DNA or RNA
◉ Which technique uses RNA, antibodies, and DNA? Answer: Hybrid
Capture Assay
◉ Use of bisulfite treatment of DNA to determine its pattern of
methylation Answer: Bisulfite DNA
◉ What tests are isothermal? Answer: NASBA, TMA, & SDA
◉ Which leukemia is associated with FLT3 gene? Answer: AML
◉ T-cell gene rearrangement is seen in what malignancy? Answer:
Sezary Syndrome
,◉ Which translocation causes Acute Premyelocytic Leukemia?
Answer: t 15;17
◉ Ewing Sarcoma Answer: t 11;22
◉ Synovial Sarcoma Answer: t X;18
◉ Follicular Lymphoma Answer: t 14;18
◉ What genes would be screened in a colorectal panel? Answer:
MSH2 & MLH1
◉ What gene mutation causes a high risk of breast or ovarian
cancer? Answer: BRCA1 and BRCA2
◉ What does MSI test for? Answer: Mutations in mismatch repair
genes (MMR)
◉ What disease is related to factor V coagulation gene (F5 1q23)?
Answer: Thrombossis
◉ How many copies are in a full Fragile X mutation? Answer: 200-
2000+ CGG repeats
, ◉ What disease is known for numerous mutations and large
deletions? Answer: Muscular Dystrophy (Duchenne)
◉ What HPV causes cervical cancer? Answer: 16 & 18
◉ You receive a paraffin embedded specimen for HPV typing, which
method should you use? Answer: PCR
◉ Significant change in HIV viral load: Answer: 0.5 log10
◉ Lowest viral concentration that can be detected over background
or a negative control in the test assay Answer: Limit of Detection
(LOD)
◉ HCV consensus sequence: Answer: G-rich Core Gene
◉ What responds poorly to ribavarin/interferon treatment? Answer:
HCV Type 1B
◉ What virus affects patients after kidney transplant? Answer: CMV
or BKV
