MB ASCP EXAM 2026 PRACTICE SCRIPT
TESTED QUESTIONS WITH VERIFIED ANSWERS
◉ DNA and histone interaction is due to what type of bond? Answer:
Ionic
◉ What stains to the minor groove of DNA duplex? Answer: SYBR
Green
◉ What is the most abundant type of RNA? Answer: Ribosomal RNA
(rRNA)
◉ What does RNA act as in a retrovirus? Answer: Genome and
mRNA
◉ What increases half-life of mRNA? Answer: 5' Methyl Cap
◉ What kind of DNA is most likely to be transcriptionally active?
Answer: Euchromatin
◉ What is the most common type of eukaryotic DNA? Answer:
Satellite DNA
,◉ What specimen has the most DNA according to molecular weight?
Answer: White Blood Cells
◉ What amino acids compose histones? Answer: Lysine and
Arginine
◉ Amino acid that all proteins start with: Answer: Methionine
◉ What protects the DNA strand during replication? Answer: Single-
stranded binding protein
◉ Leading Strand Answer: 3' to 5' template
◉ Lagging Strand Answer: 5' to 3' template
◉ What DNA strand is being copied in transcription? Answer:
Antisense Strand
◉ First step after newly formed mRNA is made? Answer: Addition of
poly(A) tail
◉ Every time the chromosome is copied it would become shorter if
not for what enzyme? Answer: Telomerase
,◉ What polymerase has repair activity? Answer: Beta/Epsilon
◉ What polymerase acts on leading strand? Answer: Delta
◉ What polymerase acts on lagging strand? Answer: Alpha
◉ Which polymerase is used for mitochondrial replication and
repair? Answer: Gamma
◉ What polymerase binds the 5' end to 3' end of dsDNA? Answer:
Topoisomerase
◉ Which RNA polymerase is mainly for transcription? Answer: RNA
Pol II
◉ Which polymerase is involved with mRNA synthesis? Answer:
RNA Pol II
◉ What inactivates RNAses? Answer: Diethyl pyrocarbonate (DEPC)
or bake glassware for 4 to 6 hours at 400C
, ◉ What type of restriction enzyme recognizes palindromic sites?
Answer: Restriction Enzyme Type II
◉ What type of restriction enzyme lacks methylation activity?
Answer: Restriction Enzyme Type II
◉ What is necessary to make cDNA? Answer: Reverse transcriptase
◉ Number of SNPs in a human genome? Answer: 11 billion (10^7)
◉ How many genes (base pairs) are in a human haploid genome?
Answer: 3 billion base pairs
◉ The polymerization of acrylamide requires: Answer: Catalyst (APS
or TEMED) or Light activation
◉ How does agarose gel pore size relate to concentration? Answer:
Inversely proportional
◉ What is denatured during a southern blot? Answer: Hydrogen
bonds (inter-strand bonds)
◉ Melting temperature formula Answer: Tm = (4C x GC Pairs) + (2C
x AT pairs)
TESTED QUESTIONS WITH VERIFIED ANSWERS
◉ DNA and histone interaction is due to what type of bond? Answer:
Ionic
◉ What stains to the minor groove of DNA duplex? Answer: SYBR
Green
◉ What is the most abundant type of RNA? Answer: Ribosomal RNA
(rRNA)
◉ What does RNA act as in a retrovirus? Answer: Genome and
mRNA
◉ What increases half-life of mRNA? Answer: 5' Methyl Cap
◉ What kind of DNA is most likely to be transcriptionally active?
Answer: Euchromatin
◉ What is the most common type of eukaryotic DNA? Answer:
Satellite DNA
,◉ What specimen has the most DNA according to molecular weight?
Answer: White Blood Cells
◉ What amino acids compose histones? Answer: Lysine and
Arginine
◉ Amino acid that all proteins start with: Answer: Methionine
◉ What protects the DNA strand during replication? Answer: Single-
stranded binding protein
◉ Leading Strand Answer: 3' to 5' template
◉ Lagging Strand Answer: 5' to 3' template
◉ What DNA strand is being copied in transcription? Answer:
Antisense Strand
◉ First step after newly formed mRNA is made? Answer: Addition of
poly(A) tail
◉ Every time the chromosome is copied it would become shorter if
not for what enzyme? Answer: Telomerase
,◉ What polymerase has repair activity? Answer: Beta/Epsilon
◉ What polymerase acts on leading strand? Answer: Delta
◉ What polymerase acts on lagging strand? Answer: Alpha
◉ Which polymerase is used for mitochondrial replication and
repair? Answer: Gamma
◉ What polymerase binds the 5' end to 3' end of dsDNA? Answer:
Topoisomerase
◉ Which RNA polymerase is mainly for transcription? Answer: RNA
Pol II
◉ Which polymerase is involved with mRNA synthesis? Answer:
RNA Pol II
◉ What inactivates RNAses? Answer: Diethyl pyrocarbonate (DEPC)
or bake glassware for 4 to 6 hours at 400C
, ◉ What type of restriction enzyme recognizes palindromic sites?
Answer: Restriction Enzyme Type II
◉ What type of restriction enzyme lacks methylation activity?
Answer: Restriction Enzyme Type II
◉ What is necessary to make cDNA? Answer: Reverse transcriptase
◉ Number of SNPs in a human genome? Answer: 11 billion (10^7)
◉ How many genes (base pairs) are in a human haploid genome?
Answer: 3 billion base pairs
◉ The polymerization of acrylamide requires: Answer: Catalyst (APS
or TEMED) or Light activation
◉ How does agarose gel pore size relate to concentration? Answer:
Inversely proportional
◉ What is denatured during a southern blot? Answer: Hydrogen
bonds (inter-strand bonds)
◉ Melting temperature formula Answer: Tm = (4C x GC Pairs) + (2C
x AT pairs)
