Samiha Tania
Id-2231434
“Lab report”
Gram staining produce
Method• Aseptically prepare air -dired , heat-fixed smear of the bacteria provided
• Flood the smears with crystal violet for 1 minute .wash with DI water . It is not necessary to
blot the slide.
• Floor the smear with gram’s Iodine for 1 minute. The mordant
binds to the crystal violet and makes it resist decolonization
then wash with DI water again
• Decolorize with 95% ethanol .the timing for this step is
something that must be learned . If the smear is not overly thick
, 5 to 10 seconds is sufficient for thicker smears ,
decolonization may need to be performed for 1 minute once or
even twice the wash with DI water .
• Flood the smear with safranin for the least 1 minute rise with
DI water and blot the smear .the slide now ready for
examination.
2 • Gram negative bacteria thin layer of peptidoglycan and
high lipid content – stains red/pink .
Observation
Id-2231434
“Lab report”
Gram staining produce
Method• Aseptically prepare air -dired , heat-fixed smear of the bacteria provided
• Flood the smears with crystal violet for 1 minute .wash with DI water . It is not necessary to
blot the slide.
• Floor the smear with gram’s Iodine for 1 minute. The mordant
binds to the crystal violet and makes it resist decolonization
then wash with DI water again
• Decolorize with 95% ethanol .the timing for this step is
something that must be learned . If the smear is not overly thick
, 5 to 10 seconds is sufficient for thicker smears ,
decolonization may need to be performed for 1 minute once or
even twice the wash with DI water .
• Flood the smear with safranin for the least 1 minute rise with
DI water and blot the smear .the slide now ready for
examination.
2 • Gram negative bacteria thin layer of peptidoglycan and
high lipid content – stains red/pink .
Observation
