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Microbiology - Unknown Identification Tests
Questions and Answers (100% Correct Answers)
Exoenzymes
Ans: Microorganisms can be identified by their _______, which digest large
macromolecules into smaller molecules
Amylase
Ans: Starch hydrolysis indicates the presence of this exoenzyme.
Lipase
Ans: Lipid hydrolysis indicates the presence of this exoenzyme.
Casease
Ans: Casein hydrolysis indicates the presence of this exoenzyme.
Gelatinase
Ans: Gelatin hydrolysis indicates the presence of this exoenzyme.
Gelatin
Ans: Gelatin deeps are used to indicate the hydrolysis of this macromolecule.
Starch
Ans: Starch plates are used to indicate the hydrolysis of this macromolecule.
Lipid
Ans: Spirit blue plates are used to indicate the hydrolysis of this macromolecule.
Casein
Ans: Milk plates are used to indicate the hydrolysis of this macromolecule.
Carbohydrates
Ans: Sugars such as glucose, lactose, and sucrose are ________ (a kind of
macromolecule).
Acid, and gas
Ans: The production of ______ , and sometimes _______ indicates the fermentation of
carbohydrates.
Durham Tube
Ans: This is used to detect the production gas in broth tubes.
Phenol Red
Ans: This is a pH indicator used to detect acid production in Lab 31 (fermentation of
sugars).
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Pink
Ans: This phenol red indicator color indicates alkalinity.
Yellow
Ans: This phenol red indicator color indicates acidity.
Beta-galactosidase
Ans: This enzyme can break down lactose.
Sucrase
Ans: This enzyme can break down sucrose.
TSI (triple sugar-iron test)
Ans: This test is used to differentiate between various gram-negative bacilli, and is
based on sugar fermentation and hydrogen sulfide gas production.
Thiosulfate Reductase
Ans: This enzyme is responsible for the production of hydrogen sulfide gas, and
breaks down thiosulfate.
Ferrous Sulfate
Ans: The addition of this salt is used to detect the presence of hydrogen sulfide gas.
Black Precipitate
Ans: In the presence of hydrogen sulfide gas, ferrous sulfate will react to form a
__________.
Large
Ans: ______ amounts of acid production is indicated when both the butt and the
slant turn yellow in a TSI test.
Glucose
Ans: If only _______ is fermented in a TSI test, then only the butt of the tube will be
yellow (the slant will be red).
Obligate aerobe
Ans: Failure to turn the butt or slant yellow in a TSI test indicates that the
microorganism was not able to ferment any of the sugars, and that it is a(n) _______.
IMViC
Ans: This is a series of tests useful in differentiating the main groups of gram-
negative bacilli in the family enterobacteriaceae.
enterobacteriaceae
Ans: The IMViC test is a series of tests useful in differentiating the main groups of
gram-negative bacilli in the family _____________.
Microbiology - Unknown Identification Tests
Questions and Answers (100% Correct Answers)
Exoenzymes
Ans: Microorganisms can be identified by their _______, which digest large
macromolecules into smaller molecules
Amylase
Ans: Starch hydrolysis indicates the presence of this exoenzyme.
Lipase
Ans: Lipid hydrolysis indicates the presence of this exoenzyme.
Casease
Ans: Casein hydrolysis indicates the presence of this exoenzyme.
Gelatinase
Ans: Gelatin hydrolysis indicates the presence of this exoenzyme.
Gelatin
Ans: Gelatin deeps are used to indicate the hydrolysis of this macromolecule.
Starch
Ans: Starch plates are used to indicate the hydrolysis of this macromolecule.
Lipid
Ans: Spirit blue plates are used to indicate the hydrolysis of this macromolecule.
Casein
Ans: Milk plates are used to indicate the hydrolysis of this macromolecule.
Carbohydrates
Ans: Sugars such as glucose, lactose, and sucrose are ________ (a kind of
macromolecule).
Acid, and gas
Ans: The production of ______ , and sometimes _______ indicates the fermentation of
carbohydrates.
Durham Tube
Ans: This is used to detect the production gas in broth tubes.
Phenol Red
Ans: This is a pH indicator used to detect acid production in Lab 31 (fermentation of
sugars).
, 2 Inquire through: | Professional | Confidential Support
Pink
Ans: This phenol red indicator color indicates alkalinity.
Yellow
Ans: This phenol red indicator color indicates acidity.
Beta-galactosidase
Ans: This enzyme can break down lactose.
Sucrase
Ans: This enzyme can break down sucrose.
TSI (triple sugar-iron test)
Ans: This test is used to differentiate between various gram-negative bacilli, and is
based on sugar fermentation and hydrogen sulfide gas production.
Thiosulfate Reductase
Ans: This enzyme is responsible for the production of hydrogen sulfide gas, and
breaks down thiosulfate.
Ferrous Sulfate
Ans: The addition of this salt is used to detect the presence of hydrogen sulfide gas.
Black Precipitate
Ans: In the presence of hydrogen sulfide gas, ferrous sulfate will react to form a
__________.
Large
Ans: ______ amounts of acid production is indicated when both the butt and the
slant turn yellow in a TSI test.
Glucose
Ans: If only _______ is fermented in a TSI test, then only the butt of the tube will be
yellow (the slant will be red).
Obligate aerobe
Ans: Failure to turn the butt or slant yellow in a TSI test indicates that the
microorganism was not able to ferment any of the sugars, and that it is a(n) _______.
IMViC
Ans: This is a series of tests useful in differentiating the main groups of gram-
negative bacilli in the family enterobacteriaceae.
enterobacteriaceae
Ans: The IMViC test is a series of tests useful in differentiating the main groups of
gram-negative bacilli in the family _____________.
