MICROBIOLOGY ASCP EXAM FINAL PAPER
2026 TESTED QUESTIONS WITH FULL
SOLUTION GRADED A+
◉ Which group of tests best identifies the Morganella and Proteus
genera? Answer: Morganella and Proteus species are motile,
produce urease and deaminate phenylalanine.
◉ Edwardsiella tarda Answer: Indole positive.
Hydrogen sulfide positive.
Sucrose negative.
V.P. Negative.
◉ Enterobacter aerogenes. Answer: Indole negative.
Hydrogen sulfide negative.
Sucrose positive.
V.P. Positive.
◉ How is Enterobacter sakazakii differentiated from Enterobacter
cloacae? Answer: By sorbitol fermentation.
Enterobacter cloacae is sorbitol positive and Enterobacter sakazakii
is sorbitol negative.
,◉ What is the difference between Klebsiella oxytoca and Klebsiella
pneumoniae? Answer: Indole production.
Klebsiella oxytoca is indole positive.
Klebsiella pneumoniae is indole negative.
◉ Will Yersenia species grow on MacConkeys agar. Answer: Yes. It
will grow small colonies at 37 degrees Celsius but will grow larger
colonies at 22 degrees
Celsius.
◉ Is E.coli indole positive or indole negative? Answer: Indole
positive.
◉ What is the oxidase reaction of Campylobacter? Answer: Oxidase
positive.
◉ Will campylobacter grow on MacConkeys? Answer: No.
◉ What is the hydrogen sulfide reaction of Salmonella? Answer:
Hydrogen sulfide positive.
,◉ What is the urease reaction of proteus? Answer: Urease positive.
◉ What two diarrheas cause blood diarrhea? Answer:
Campylobacter and Shigella.
◉ What media is the best choice for the isolation of Yersenia
species? Answer: C.I.N. - cefsulodin-irgasan-novobiocin medium.
◉ What is the drugs of choice for the treatment of infections of
Enterobacteriaceae? Answer: Aminoglycosides.
Trimethoprim-sulfamethoxazole.
Third generation cephalosporins.
◉ What is the Shiga-like toxin called and which Enterobacteriaceae
is it produced by? Answer: Verotoxin.
It is produced by E.coli.
◉ What is the Kauffman White Classiffication? Answer: The system
that classifies the salmonellae on the basis of the somatic O - heat
soluble - antigens and subdivides them into serotypes base on their
flagella H - heat labile - antigens.
, ◉ What is the TSI for Klebsiella pneumoniae? Answer: A/A: acid
slant/ acid butt.
◉ What are the biochemical reactions of Klebsiella pneumoniae?
Answer: Motility - negative.
Methyl red - negative.
Hydrogen sulfide- negative.
◉ What are the biochemical reactions of E. coli? Answer: TSI -
acid/acid gas
VP - negative
Hydrogen sulfide - negative
Urease - negative
Indole - positive
Methyl red - positive.
Citrate - negative.
Lysine decarboxylase - positive.
◉ What are the most appropriate screening tests to presumptively
differentiate and identify the nonfermentative gram negative bacilli
(NFB) from the Enterobacteriaceae? Answer: Oxidase - All
Enterobacteriaceae are positive but not all N.F.B are oxidase
variable.
2026 TESTED QUESTIONS WITH FULL
SOLUTION GRADED A+
◉ Which group of tests best identifies the Morganella and Proteus
genera? Answer: Morganella and Proteus species are motile,
produce urease and deaminate phenylalanine.
◉ Edwardsiella tarda Answer: Indole positive.
Hydrogen sulfide positive.
Sucrose negative.
V.P. Negative.
◉ Enterobacter aerogenes. Answer: Indole negative.
Hydrogen sulfide negative.
Sucrose positive.
V.P. Positive.
◉ How is Enterobacter sakazakii differentiated from Enterobacter
cloacae? Answer: By sorbitol fermentation.
Enterobacter cloacae is sorbitol positive and Enterobacter sakazakii
is sorbitol negative.
,◉ What is the difference between Klebsiella oxytoca and Klebsiella
pneumoniae? Answer: Indole production.
Klebsiella oxytoca is indole positive.
Klebsiella pneumoniae is indole negative.
◉ Will Yersenia species grow on MacConkeys agar. Answer: Yes. It
will grow small colonies at 37 degrees Celsius but will grow larger
colonies at 22 degrees
Celsius.
◉ Is E.coli indole positive or indole negative? Answer: Indole
positive.
◉ What is the oxidase reaction of Campylobacter? Answer: Oxidase
positive.
◉ Will campylobacter grow on MacConkeys? Answer: No.
◉ What is the hydrogen sulfide reaction of Salmonella? Answer:
Hydrogen sulfide positive.
,◉ What is the urease reaction of proteus? Answer: Urease positive.
◉ What two diarrheas cause blood diarrhea? Answer:
Campylobacter and Shigella.
◉ What media is the best choice for the isolation of Yersenia
species? Answer: C.I.N. - cefsulodin-irgasan-novobiocin medium.
◉ What is the drugs of choice for the treatment of infections of
Enterobacteriaceae? Answer: Aminoglycosides.
Trimethoprim-sulfamethoxazole.
Third generation cephalosporins.
◉ What is the Shiga-like toxin called and which Enterobacteriaceae
is it produced by? Answer: Verotoxin.
It is produced by E.coli.
◉ What is the Kauffman White Classiffication? Answer: The system
that classifies the salmonellae on the basis of the somatic O - heat
soluble - antigens and subdivides them into serotypes base on their
flagella H - heat labile - antigens.
, ◉ What is the TSI for Klebsiella pneumoniae? Answer: A/A: acid
slant/ acid butt.
◉ What are the biochemical reactions of Klebsiella pneumoniae?
Answer: Motility - negative.
Methyl red - negative.
Hydrogen sulfide- negative.
◉ What are the biochemical reactions of E. coli? Answer: TSI -
acid/acid gas
VP - negative
Hydrogen sulfide - negative
Urease - negative
Indole - positive
Methyl red - positive.
Citrate - negative.
Lysine decarboxylase - positive.
◉ What are the most appropriate screening tests to presumptively
differentiate and identify the nonfermentative gram negative bacilli
(NFB) from the Enterobacteriaceae? Answer: Oxidase - All
Enterobacteriaceae are positive but not all N.F.B are oxidase
variable.
